Immobilization of glucose oxidase on polymer membranes treated by low‐temperature plasma

Abstract

Low-temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a linking agent. Enzyme activity was evaluated by the response of glucose sensor composed of the immobilized enzyme membrane and a dissolved oxygen electrode. The sensor response was found to depend on the kind of carrier membrane and to become maximum at suitable conditions of plasma treatment.

A ToF-SIMS study of the deuterium—hydrogen exchange induced by ammonia plasma treatment of polyolefins

“…Specifically in the biotech community this plasma approach had been exploited and studied in the past. Examples are the promotion of cellaffinity 1,2 and immobilization capabilities for albumin, 3 glucose oxidase, 4 or enzyme. 5 Surface chemical characterization by photoelectron spectroscopy (XPS) [6][7][8] and time-of-flight secondary ion mass spectrometry (ToF-SIMS) 9 is a highly relevant approach to optimize the amine layer functionality and to assure its quality for applications.…”

Biomedical Applications of Plasma-Deposited Thin Films

“…However, only biosensor applications of plasma polymers shall be considered in this section and for a more detailed discussion of their use in the fabrication of sensitive layers, the reader is directed to a recent review publication [75]. The potential of RF plasma for biosensor devices was investigated using N2 and NH3 to modify propylene (DuragardB), poly(viny1idene fluoride) (DuraporeB) and poytetrafluoroethylene (FluroporeB) membrane surfaces [76]. Subsequently this allowed immobilization of glucose oxidase (GOD) using glutaraldehyde as a linking agent for covalent attachment.…”

Chemical Immobilization of Biomolecules on Plasma‐Modified Substrates for Biomedical Applications

“…Glucose oxidase has been immobilized onto various polymeric membranes (polypropylene, PP, PTFE, poly(vinylidene fluoride), PVDF) that have been plasma modified with surface amino groups [56]. Membranes have been treated in NH 3 -and N 2 -fed glow discharges and the immobilization of the enzyme has been performed via a glutaraldehyde-aided reaction.…”