Immobilization of naringinase from Aspergillus niger CECT 2088 in poly(vinyl alcohol) cryogels for the debittering of juices

Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

“…High stabilization by the restriction of the movement of the enzyme immobilized in the beads could cause the increase of the optimum temperature for the immobilized enzyme. Similar results were found for phospholipase A1 in gelatin hydrogel (Sheelu et al, 2008) and other enzymes immobilized in matrices (Altun and Cetinus, 2007;Busto et al, 2007). Fig.…”

“…In fact, the immobilized enzyme lost almost half of its initial activity after eight cycles of operation. Data on operational stability from previously reported studies involving PVA immobilization of naringinase showed that only 36% of the initial activity was retained after six reuses (Busto et al, 2007). Fig.…”

Optimization, production and scale up of debittered kinnow beverage by and #945;-L-rhamnosidase producing yeast

“…Naringin (4′,-5,7′-trihydroxyflavonone-7-rhamnoglucoside) is first hydrolyzed by α-L-rhamnosidase activity of naringinase to rhamnose and prunin (one third of the bitterness of naringin) which can be further hydrolyzed into glucose and naringenin by the β-D-glucosidase component of naringinase. The potential application of rhamnosidase is used in the debittering of citrus fruit juices (Busto et al, 2007), manufacture of prunin from naringin, manufacture of L-rhamnose by hydrolysis of natural glycosides containing terminal L-rhamnose, enhancement of wine aromas by enzymatic hydrolysis of terpenyl glycosides containing L-rhamnose, elimination of hesperidin crystals from orange juices, conversion of chloropolysporin B to chloropolysporin C, the derhamnosylation of many L-rhamnose containing steroids for example, diosgene, desglucoruscin, ginsenosides-Rg2, etc. whose derhamnosylated products have their clinical importance (Feng et al, 2005).…”

A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

“…2 Polyvinyl alcohol-based matrices have been shown to lead to particles with considerable chemical and mechanical stability, providing a suitable alternative to overcome the drawbacks of natural polymers while retaining the benefits inherent to their use. 3,4 Several methods have been developed to form hydrogels from polyvinyl alcohol (PVA), namely freeze-thawing, 5,6 irradiation 3 or chemical cross-linking. 7 -9 A different approach, which has reached commercial application, relies on the controlled drying of a PVA-based hydrogel (LentiKat  Liquid), and subsequent chemical stabilization, leading to lens-shaped particles.…”