Immunocytochemical evidence that neurotensin is present in glutamatergic neurons in the superficial dorsal horn of the rat

Todd, Andrew; Spike, Rosemary C.; Price, RF; Neilson, M

doi:10.1523/jneurosci.14-02-00774.1994

Cited by 62 publications

(36 citation statements)

References 45 publications

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“…In situ hybridization demonstrated that this was indeed the case, and PACAP expression was essentially abolished in the dorsal horn of Tlx1/3 deletion mice (data not shown). In addition, because the peptide NT was mainly expressed in glutamatergic neurons in the dorsal spinal cord (Todd et al, 1994), we then examined whether Tlx1/3 are required for its expression. In situ hybridization results revealed that the expression of NT was also compromised in the Tlx1/3 mutant mice (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord

Guo

Zhao²,

Huang³

et al. 2012

Journal of Neuroscience

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Establishing the pattern of expression of transmitters and peptides as well as their receptors in different neuronal types is crucial for understanding the circuitry in various regions of the brain. Previous studies have demonstrated that the transmitter and peptide phenotypes in mouse dorsal spinal cord neurons are determined by the transcription factors Tlx1/3 and Ptf1a. Here we show that these transcription factors also determine the expression of two distinct sets of transmitter and peptide receptor genes in this region. We have screened the expression of 78 receptor genes in the spinal dorsal horn by in situ hybridization. We found that receptor genes Gabra1, Gabra5, Gabrb2, Gria3, Grin3a, Grin3b, Galr1, and Npy1r were preferentially expressed in Tlx3-expressing glutamatergic neurons and their derivatives, and deletion of Tlx1 and Tlx3 resulted in the loss of expression of these receptor genes. Furthermore, we obtained genetic evidence that Tlx3 uses distinct pathways to control the expression of receptor genes. We also found that receptor genes Grm3, Grm4, Grm5, Grik1, Grik2, Grik3, and Sstr2 were mainly expressed in Pax2-expressing GABAergic neurons in the spinal dorsal horn, and their expression in this region was abolished or markedly reduced in Ptf1a and Pax2 deletion mutant mice. Together, our studies indicate that Tlx1/3 and Ptf1a, the key transcription factors for fate determination of glutamatergic and GABAergic neurons in the dorsal spinal cord, are also responsible for controlling the expression of two distinct sets of transmitter and peptide receptor genes.

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Section: Resultsmentioning

confidence: 99%

Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord

Guo

Zhao²,

Huang³

et al. 2012

Journal of Neuroscience

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“…Todd et al (1994) reported that a population of glutamate immunoreactive boutons in superficial dorsal horn contained neurotensin, a neuropeptide with predom- Fig. 8.…”

Section: Glutamate and Enkephalin As Cotransmitters In Dorsal Horn Nementioning

confidence: 99%

Morphology and electrophysiological properties of hamster spinal dorsal horn neurons that express VGLUT2 and enkephalin

Schneider

Walker

2007

J of Comparative Neurology

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The excitatory amino acid glutamate mediates transmission at spinal synapses, including those formed by sensory afferent fibers and by intrinsic interneurons. The identity and physiological properties of glutamatergic dorsal horn neurons are poorly characterized despite their importance in spinal sensory circuits. Moreover, many intrinsic spinal glutamatergic synapses colocalize the opioid peptide enkephalin (ENK), but the neurons to which they belong are yet to be identified. Therefore, we used immunohistochemistry and confocal microscopy to investigate expression of the VGLUT2 vesicular glutamate transporter, an isoform reported in nonprimary afferent spinal synapses, and ENK in electrophysiologically identified neurons of hamster spinal dorsal horn. VGLUT2 immunoreactivity was localized in restricted fashion to axon varicosities of neurons recorded from laminae II-V, although the occurrence of immunolabeling in individual varicosities varied widely between cells (39 +/- 36%, n = 31 neurons). ENK colocalized with VGLUT2 in up to 77% of varicosities (17 +/- 21%, n = 21 neurons). The majority of neurons expressing VGLUT2 and/or ENK had axons with dense local terminations or projections consistent with propriospinal functions. VGLUT2 and ENK labeling were not correlated with cellular morphology, intrinsic membrane properties, firing patterns, or synaptic responses to sensory afferent stimulation. However, VGLUT2 expression was significantly higher in neurons with depolarized resting membrane potential. The results are new evidence for a population of dual-function dorsal horn interneurons that might provide another mechanism for limiting excitation within dorsal horn circuits during periods of strong sensory activation.

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“…Somatostatin and its receptors are expressed in a subset of small DRG neurons and activation of peripheral somatostatin receptors reduces inflammatory nociceptive behavior (Hökfelt et al, 1976;Carlton et al, 2001;Bar et al, 2004). Moreover, low levels of galanin, neurotensin, and neuropeptide Y are expressed in DRG neurons under normal circumstance, in addition to their expression in the spinal dorsal horn neurons (Todd et al, 1994;Simmons et al, 1995;X. Zhang et al, , 1998Polgar et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Inflammatory Pain by Activating B-Type Natriuretic Peptide Signal Pathway in Nociceptive Sensory Neurons

Zhang¹,

Liu²,

Gong³

et al. 2010

J. Neurosci.

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B-type natriuretic peptide (BNP) has been known to be secreted from cardiac myocytes and activate its receptor, natriuretic peptide receptor-A (NPR-A), to reduce ventricular fibrosis. However, the function of BNP/NPR-A pathway in the somatic sensory system has been unknown. In the present study, we report a novel function of BNP in pain modulation. Using microarray and immunoblot analyses, we found that BNP and NPR-A were expressed in the dorsal root ganglion (

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Immunocytochemical evidence that neurotensin is present in glutamatergic neurons in the superficial dorsal horn of the rat

Cited by 62 publications

References 45 publications

Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord

Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord

Morphology and electrophysiological properties of hamster spinal dorsal horn neurons that express VGLUT2 and enkephalin

Inhibition of Inflammatory Pain by Activating B-Type Natriuretic Peptide Signal Pathway in Nociceptive Sensory Neurons

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