Immunoglobulin subclass in experimental murine Toxocara cati infection

Abstract

Aim:The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.Materials and Methods:The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.Results:The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.Conclusion:Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Culling Layer Hen Gastrointestinal Helminth Identification at Wonokromo Market Surabaya

“…This disease could be prevented by improving cage sanitation, cleanliness of the surrounding environment, maintenance management and regular insecticide spraying. Treatments that usually given to treat nematode worms in the digestive tract of laying hens include piperazine, levamisole and phenothiazine, and to treat cestode nematode worm infections in the digestive tract of laying hens can be given albendazole (Kusnoto et al, 2017).…”

Identification and Distribution of Soil Transmitted Helminths around The Shed and Grazing Fields of Madura Cattle in Sub-District of Geger, Bangkalan Regency

“…The eggs of Ancylostoma sp. worms have the characteristics of oval-shaped, thin-walled consisting of 2 layers, measuring 66.8 x 28.3 m, when released, the eggs have segments consisting of 8-16 cells (D) (Kusnoto et al, 2014) Based on the results of the study, the contamination of Soil Transmitted Helminths around the cage was Strongyloides sp., followed by Toxocara sp., and Trichuris sp. The two most contaminated species were Strongyloides sp., and Toxocara sp., followed by Strongyloides sp., and Trichuris sp.…”

“…The percentage of Soil Transmitted Helminths contamination in this study was 58%, this percentage is higher than the research conducted by Paller and Emmanuel (2014) on rural soil samples in the Philippines which showed a percentage of 31%. This could be due to the fact that the sampling was carried out in August at the beginning of the rainy season, the environmental and soil conditions became moist, so the percentage became higher (Kusnoto et al, 2014). In addition, humid areas are good conditions for the growth of various types of worms including the Soil Transmitted Helminths group to continue their life cycle (Suriptiastuti, 2006).…”

“…worms needs to be watched out for because Trichuris sp. infection can cause hemorrhagic necrosis, inflammation of the cecum mucosa, hemorrhagic diarrhea, and anemia (Kusnoto et al, 2014). Ancylostoma sp., infects dogs and cats, this worm belongs to the class of Soil Transmitted Helminths.…”

Reactivity of recombinant Toxocara canis TES‐30/120 in experimentally infected mice

“…In humans, recombinant proteins have been widely studied for toxocariasis diagnostic, with sensibility and specificity around 70%‐100% and 92%‐100%, respectively . By contrast, there is scarce information in animal studies, especially in mice, where the standard diagnostic methods are still utilized to study various infection paradigms, such as humoral immune response …”