Immunohistochemical staining of the human forebrain with monoclonal antibody to human choline acetyltransferase

Nagai, Toshisaburo; Pearson, Terry W.; Peng, Frank; McGeer, E.G.; McGeer, P. L.

doi:10.1016/0006-8993(83)90346-3

Cited by 69 publications

(6 citation statements)

References 16 publications

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“…Basal forebrain Our observation on the morphology and topographical localization of ChAT-positive perikarya is similar to those in previous studies on the rat Houser et al, 1983;Kimura et al, 1981b;Satoh et al, 1983: Sofroniew et al, 1982, and is compatible with those on the cat (Kimura et al, 1981a), primate (Hedreen et al, 1983;Mesulam et al, 1984), and human (Nagai et al, 1983). We found no ChAT-positive structure in the neuropil, although Kimura et al (1981a) reported that ChAT-positive dots are attached to the surfaces of ChAT-positive and -negative perikarya in the cat.…”

Section: Olfactory Brainsupporting

confidence: 91%

“…The distribution of the cholinergic perikarya in the mammalian CNS has been established immunohistochemically using antisera (Kimura et al, 198 la, b;Sofroniew et al, 1982) or monoclonal antibodies Hedreen et al, 1983;Houser et al, 1983;Mesulam et al, 1984;Nagai et al, 1983;Satoh et al, 1983) to ChAT. ChAT immunohistochemistry has revealed that the histochemical demonstration of AChE, which has been widely used for studying cholinergic neurons, can be adopted only in some regions in the CNS (Eckenstein and Sofroniew, 1983;Levey et al, 1983;Mesulam et al, 1984;Satoh et al, 1983).…”

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confidence: 99%

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Organization of choline acetyltransferase-containing structures in the forebrain of the rat

Ichikawa¹,

Hirata²

1986

J. Neurosci.

168

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The cholinergic structures in the forebrain of the rat were studied immunohistochemically with a monoclonal antibody to choline acetyltransferase (ChAT) which has proven to be particularly suited to reveal the trajectory of ChAT-positive fibers and their terminations. The habenulo-interpeduncular tract contained the most distinctly ChAT- positive fibers. Here, ChAT-positivity was demonstrated in the perikarya, fibers and terminal field. From the basal forebrain, ChAT- positive fiber bundles could be followed rostrally to the olfactory bulb, dorsomedially to the neocortex and hippocampus, and dorsocaudally to the hippocampus. Terminations of ChAT-positive fibers in these regions were either boutons terminaux (in the interpeduncular nucleus), finely granular (in the hippocampus) or a network of varicose fibers (in the olfactory bulb and neocortex). Cholinergic terminal fields were also demonstrated in the following regions: the nucleus of the lateral olfactory tract, the olfactory tubercle, the pyriform cortex, the lateral nucleus and part of the basal nucleus of the amygdala, the caudate-putamen, the nucleus accumbens, some thalamic nuclei, and the suprachiasmatic nucleus of the hypothalamus. In most of these regions, ChAT-positivity appeared as either fine granules or a meshwork of varicose fibers.

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Section: Olfactory Brainsupporting

confidence: 91%

mentioning

confidence: 99%

Organization of choline acetyltransferase-containing structures in the forebrain of the rat

Ichikawa¹,

Hirata²

1986

J. Neurosci.

168

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“…A small percentage of the neurons were large neurons with an important cytoplasm and visible processes (Fig. 11) that most probably correspond to the large cholinergic neurons previously characterized in the human adult (Nagai et al, 1983).…”

Section: General Data and Control For Specificity Of Ishmentioning

confidence: 98%

Ontogeny of the striatal neurons expressing neuropeptide genes in the human fetus and neonate

Brana

Charron

Aubert

et al. 1995

J of Comparative Neurology

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The distribution patterns of neurons expressing mRNAs for four neuropeptides in the human striatum were studied during ontogeny by the use of in situ hybridization. The results of our study demonstrate that somatostatin, enkephalin, dynorphin, and substance P mRNAs are present in striatal neuronal populations from week 12 of fetal life. Each neuronal population undergoes a specific differentiation. Neurons containing somatostatin mRNA are scattered throughout the caudate-putamen up until birth. Neurons containing enkephalin, dynorphin, or substance P mRNAs evolve throughout fetal life in relation to caudate-putamen and patch-matrix compartmentalization. Neurons containing enkephalin mRNA (distinct from those containing substance P or dynorphin mRNAs) are present in the matrix from week 12 of fetal life. These neurons are preferentially distributed in the matrix and, at birth, display higher enkephalin mRNA content in the matrix than in the patches. Dynorphin mRNA is found in the caudate and putamen, preferentially in the patch neurons; nevertheless, a low level of dynorphin mRNA is also present in neurons of the caudate matrix. Substance P mRNA is initially restricted to caudate neurons. At birth, both substance P and dynorphin mRNAs are expressed at high levels in the patches. These results demonstrate that each neuropeptide gene is expressed during human fetal life in neurons with a specific topology and pace of development in relation to caudate-putamen and patch-matrix differentiation. These results also contribute evidence that neurochemical evolution of the striatal neuronal populations is not complete at birth in humans.

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“…The distribution of ChAT-positive fibers in the hippocampal formation has been investigated in a number of studies involving rodents (e.g., Mesulam et al, 1983b;Frotscher and Leranth, 1985;Wainer et al, 1985;Blaker et al, 1988;Lysakowski et al, 1989); carnivores (Kimura et al, 1981;Vincent and Beiner, 1987); different species of monkeys: baboon (Satoh and Fibiger, 1985a,b), rhesus macaque (Mesulam et al, 1983aKitt et al, 19871, Cebus monkey (Kordower et al, 1989); and humans (Nagai et al, 1983;Hedreen et al, 1985;Saper and Chelimsky, 1984;Ransmayr et al, 1989Ransmayr et al, , 1992Lim et al, 1991;Mesulam et al, 1992;LehBricy et al, 1993). The general distribution of cholinergic fibers and terminals that we observed was fundamentally similar to that previously reported in other species of primates but different in a number of significant ways from the distribution reported for rodents.…”

Section: Interspecies Differences In the Cholinergic Innervation Of Tmentioning

confidence: 99%

Cholinergin innervation of the primate hippocampal formation. I. Distribution of choline acetyltransferasse immunoreactivity in the Macaca fascicularis and Macaca mulatta monkeys

Alonso

Amaral

1995

J of Comparative Neurology

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The cholinergic innervation of the hippocampal formation of Macaca fascicularis (cynomolgus) and Macaca mulatta (rhesus) monkeys was investigated by immunohistochemical procedures using a monoclonal antibody directed against choline acetyltransferase. The distribution of choline acetyltransferase in the monkey demonstrated both similarities and differences with the staining patterns observed in the rat or with acetylcholinesterase in the monkey. While both of these latter preparations demonstrated labeled cells, for example, no choline acetyltransferase labeled neurons were observed in the monkey hippocampal formation. Choline acetyltransferase activity was restricted to fibers which varied in thickness and number of varicosities and in their regional and laminar distribution. The highest densities of labeled fibers were observed in the uncal portion of the hippocampus, in the parasubiculum, and in the entorhinal cortex; the lowest densities of labeled fibers were observed in CA1 and in midrostrocaudal levels of the dentate gyrus. In the dentate gyrus, immunoreactive fibers were densely distributed in the molecular layer and in an infragranular plexus. One of the few striking noticeable interspecies differences was observed in the dentate gyrus. In the rhesus monkey, labeled fibers in the molecular layer were divided into a superficial denser and an inner lighter lamina, whereas in M. fascicularis, the cholinergic fibers were distributed more homogeneously throughout the molecular layer. In the hippocampus proper, there was a progressive decrease in the density of ChAT-immunoreactive fibers from CA3/CA2 into CA1. The subiculum also demonstrated modest labeling which was nonetheless higher than in CA1; the border of these fields demonstrated increased fiber labeling. The density of choline acetyltransferase staining was high in the presubiculum and parasubiculum. In the entorhinal cortex, a relatively clear boundary was observed between the more heavily stained superficial layers (I, II, and III) and the more weakly labeled deep layers (V and VI), especially in the intermediate and caudal fields. A transverse decreasing gradient was observed with the densest plexus of cholinergic fibers found in the medially situated olfactory field of the entorhinal cortex and the lowest density in the laterally located caudal and lateral fields.

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Immunohistochemical staining of the human forebrain with monoclonal antibody to human choline acetyltransferase

Cited by 69 publications

References 16 publications

Organization of choline acetyltransferase-containing structures in the forebrain of the rat

Organization of choline acetyltransferase-containing structures in the forebrain of the rat

Ontogeny of the striatal neurons expressing neuropeptide genes in the human fetus and neonate

Cholinergin innervation of the primate hippocampal formation. I. Distribution of choline acetyltransferasse immunoreactivity in the Macaca fascicularis and Macaca mulatta monkeys

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