Immunohistochemical study of tumor necrosis factor-alpha(TNF- α) expression in lung, liver, and spleen during aspergillosis infection

Dheeb, Batol Imran

doi:10.1186/1471-2164-15-s2-p71

Cited by 13 publications

(12 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ISSR less primers means less time, less DNA, less supplied and less samples, RAPD markers don't have the specific target comparing to ISSR markers. In fact ISSR markers are known to be more sensitive than RAPD markers, in this study it was obvious that the dendrogram based on RAPD markers was not in according with the dendrogram based on ISSR markers, thus, both dendrogram are in agreement with the groups of geographic origin, but RAPD markers greatly agree with these group than ISSR markers, the differences in clustering pattern of genotypes using RAPD and ISSR markers also may be attributed to markers sampling error and the level of polymorphic detected [16,25,26].…”

Section: Issr-pcr Analysismentioning

confidence: 68%

“…program was used to determine molecular size based on comparing the RAPD-PCR and ISSR-PCR products depending on molecular size of bands and number bands of a 2000bp DNA ladder Bioneer (which consist of 13 bands from 100 to 2000 bp.) Estimation of polymorphism, efficiency and discriminatory power Data generated for molecular weight RAPD and ISSR markers result bands were a score for each bands on the molecular size (1 for present, 0 for absence) the commercial soft word [16,17]. Only major bands consistently amplified were scored.…”

Section: Data Analysis Estimation Of Molecular Weightmentioning

confidence: 99%

See 1 more Smart Citation

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

Mezher¹,

Mohammad²,

Wahab³

et al. 2018

JoBRC

View full text Add to dashboard Cite

The present study included detection of genetic variability, and identification of genetic relationship and finding a fingerprint of the ten clinical isolates related to Trichophyton rubrum using RAPD and ISSR markers. The experiment was carried out and the results performed using six primer of the RAPD markers these primers showed 239 amplified band , out of these band 90 of them was considered as a main band, and 149 was Polymorphic band , the largest number of bands was 30 in the isolate TR6 and less number of bands in the isolate TR7. The results clear the value of genetic diversity based on RAPD analysis the lowest value of genetic diversity (0.13005) between the isolate TR3 and TR9while the highest genetic diversity (0.55941) was between the isolates TR5 and TR8. The analysis of the relationship shows that there are three main groups: the first group include isolate TR8, while the second group included three isolates are isolate TR2, isolate TR10 and isolates TR3, The third group included three subgroups included the first isolate TR1,isolates TR4 and second isolate TR3 and isolate TR9 and the third included the isolate TR5 and isolates TR6.The results of ISSR experiments: the use six a primers in the of the ISSR showed 192 bands, in the isolate of Trichophyton rubrum, two of these primers showed monomorphic bands (in number and location) and six primers showed monomorphic and polymorphic bands, while one showed only polymorphic bands among Trichophyton rubrum isolates. And the largest number of bands was 24 in the TR5 and less number of bands 16 in isolate TR3 and isolate TR8 were finding DNA fingerprint to isolate TR1, isolate TR5, and isolate TR3. The ISSR markers showed lowest genetic polymorphism was (0.05561) between the isolates TR2 and isolate TR7 and the largest genetic distance was (0.40501) between the isolate TR4 and isolate TR8. The analysis of the relationship of genetic showed that there are three groups key first included isolates and only one isolate TR8 and the second involving isolates TR2 and isolate TR7, isolate TR4 and isolate TR3, while the group included three sub-groups, the first included isolate TR1 and isolate TR6, and the second involving isolate TR5, isolate TR10 and isolate TR9. The results confirmed the efficiency markers of the RAPD and ISSR in contrast, find a genetic fingerprint to the isolates of Trichophyton rubrum, and these markers differ in mechanical detection contrast, and coverage is Genome. Therefore Complementary to each other, although the ISSR markers were more efficient in terms of the number of binding sites in each type of the isolates of Trichophyton rubrum and so can the initiator of the discovery of a much larger area from Genome.

show abstract

Section: Issr-pcr Analysismentioning

confidence: 68%

Section: Data Analysis Estimation Of Molecular Weightmentioning

confidence: 99%

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

Mezher¹,

Mohammad²,

Wahab³

et al. 2018

JoBRC

View full text Add to dashboard Cite

show abstract

“…Six of RAPD primers were utilized in this study, the primers were synthesized by (Bioneer-Korea) in lyophilized type and dissolved in sterile water to obtain a final concentration of (10pmol/ml), [19,20,21]. The primers and their sequences are listed in Table (2).…”

Section: Rapd Assaymentioning

confidence: 99%

RAPD and ISSR analyses of Saccharomyces cerevisiae isolates from different sources

Hammadi¹,

Hussein²,

Majeed³

et al. 2018

JoBRC

View full text Add to dashboard Cite

The purpose of this study was to isolate the Saccharomyces cerevisiae present on different fruits and performing RAPD and ISSR analyses to know the genetic interrelationship between different S. cerevisiae isolates. Some fruits namely apple, plum, dates, and peach were used as natural sources for S. cerevisiae isolation. The isolated S. cerevisiae was designated as SUC1, SUC2, SUC3, SUC4, SUC5 respectively. Amplicon fingerprints for the isolated species were obtained by RAPD assay using six different primers and ISSR assay using six different primers. RAPD assay showed the lowest genetic distance (0.1559) between SUC2 and SUC3 isolates whereas ISSR assay showed the lowest genetic distance (0.06899) between SUC4 and SUC5 isolates. Both genetic markers showed the highest genetic distance for SUC1 when compared to the other isolates.

show abstract

“…The assessment system used for TNF-α is the intensity parameter of staining nucleus and the percentage of cells that gives positive expression [29]. The correlation between TNFα and the number of size of damage to the infected tissue was statistically tested One-Way ANOVA with BNJ test showed a significant difference (p<0.05) between cells and the intensity of the percentage of TNF-α expression shown in Table 3.…”

Section: Effects Of Resin Essential Oils Of Canarium Tree On Tnf-α Exmentioning

confidence: 99%

Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus)

Mbabho

Aulanni’am

Srihardyastutie

2018

J. Pure App. Chem. Res.

View full text Add to dashboard Cite

Essential oils of canarium tree resin (Canarium indicum L) have anti-inflammatory and antioxidant activity. The purpose of this study was to investigate the potential of essential oils of canarium tree resin on the expression of TNF-α, changes in bronchial epithelial cell features, MDA levels and protease activity on asthma rats. This study used four groups of rats, the negative control group, asthma group, therapy group dose of 25 mg/kg body weight, 50 mg/kg body weight and 100 mg/kg body weight. The ashtma rats were prepared by OVA and LPS. TNF-α expression was calculated using Walter calculation, MDA level was measured using Thiobarbituric Acid (TBA), measurement of protease activity was done by spectrophotometric method, and all data analyzed by ANOVA. Histopathologic observation of bronchi was done using Olympus BX51 microscope. The results showed that the therapy of essential oils of canarium tree resin of 25 mg/kg body weight, 50 mg/kg body weight and 100 mg/kg body weight significantly (p<0.05) decreased, MDA up to 54%, protease activity up to 46%, improved bronchial epithelium and TNF-α expression up to 67%. This study proves that essential oils of canarium tree resin can be effectively used as therapy on asthma rats.

show abstract

Immunohistochemical study of tumor necrosis factor-alpha(TNF- α) expression in lung, liver, and spleen during aspergillosis infection

Cited by 13 publications

References 1 publication

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

RAPD and ISSR analyses of Saccharomyces cerevisiae isolates from different sources

Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus)

Contact Info

Product

Resources

About