The purpose of this study was to isolate the Saccharomyces cerevisiae present on different fruits and performing RAPD and ISSR analyses to know the genetic interrelationship between different S. cerevisiae isolates. Some fruits namely apple, plum, dates, and peach were used as natural sources for S. cerevisiae isolation. The isolated S. cerevisiae was designated as SUC1, SUC2, SUC3, SUC4, SUC5 respectively. Amplicon fingerprints for the isolated species were obtained by RAPD assay using six different primers and ISSR assay using six different primers. RAPD assay showed the lowest genetic distance (0.1559) between SUC2 and SUC3 isolates whereas ISSR assay showed the lowest genetic distance (0.06899) between SUC4 and SUC5 isolates.  Both genetic markers showed the highest genetic distance for SUC1 when compared to the other isolates.

Microbes are infectious agents that are invisible to the naked eye and are found all over the world. Some of them cause sickness in humans. Bacteria, fungi, viruses, prions, and protozoa are the different types. Microscopic parasites are also included. The microbiology laboratory’s diagnosis of infection serves two major purposes: clinical and epidemiological. Antibiotics function by reducing the growth of the infecting organism and are used to treat infectious diseases both therapeutically and prophylactically. Some are ‘broad spectrum,’ which means they function against a wide range of pathogenic bacteria, while others are more specific. Despite a large number of antibiotics available, many infecting and disease-causing bacteria remain resistant to them. Many diseases of the developing world fall into this group. Evidence for a protective effect Treatment of normal animals with antibiotics reduces or removes their commensal bacteria and this allows pathogens to infect mucous surfaces more easily. In humans, after treatment with antibiotics for various diseases, secondary infections arise, e.g. oral and vaginal thrush due to Candida albicans, pseudomembranous colitis due to Clostridium difficile, and nasal infections with Staphylococcus aureus.