Immunometabolic regulation by triterpenes of Eucalyptus tereticornis in adipose tissue cell line models

Ceballos, Susana; Guillen, Alis; Muñoz, Diego A.; Castaño, Adriana; Echeverri, Fernando; Acı́n, Sergio; Balcazar, Norman

doi:10.1016/j.phymed.2018.03.059

Cited by 12 publications

(19 citation statements)

References 20 publications

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“…It was observed that in treated animals, the TNF-α, MCP-1, IL-6, and IL-1β expression is significantly reduced at a transcriptional level (see Figure 4A-D). These results match those obtained by Ceballos et al 2018, who reported a significant reduction in the expression of proinflammatory genes in J774 mouse cells treated with a triterpene mixture [27]. Studies conducted by Patil et al, 2015, validated the effect of pentacyclic triterpenes in nuclear factor kappa B (NF-kB) activation, which is a canonical pathway for the activation of proinflammatory cytokines, such as TNF-α and IL-1β, which have an important role in the genetic expression of other proinflammatory cytokines [64].…”

Section: Biological Activity Assessmentsupporting

confidence: 91%

“…These results suggest that the effect in the reduction in adipocyte leptin synthesis is independent of the effect of this compound in β cells (insulin increase) and hepatocytes (decrease TAG synthesis). Recent studies have reported a decrease in leptin synthesis following the treatment of differentiated 3T3-L1 adipocytes, with a triterpene mixture [27]. It will be worthy to determine whether decreased leptin levels in animals treated with SA is owing to a local effect inhibiting leptin synthesis in adipocytes or whether the effect is systemic, where hormone sensitivity is increased.…”

Section: Biological Activity Assessmentmentioning

confidence: 99%

“…Consumption of these naturally occurring substances seems to be associated with the prevention of metabolic syndrome and T2DM [17][18][19][20][21][22]. In vitro and animal studies have demonstrated the potential of PTs to modulate diseases ranging from diabetes to cardiovascular conditions [23][24][25][26][27][28]. Interestingly, most of the PT studied so far, exhibit anti-inflammatory, antioxidant, anti-obesity, and antidiabetic effects.…”

Section: Introductionmentioning

confidence: 99%

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Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

et al. 2020

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Plant extracts from Cecropia genus have been used by Latin-American traditional medicine to treat metabolic disorders and diabetes. Previous reports have shown that roots of Cecropia telenitida that contains serjanic acid as one of the most prominent and representative pentacyclic triterpenes. The study aimed to isolate serjanic acid and evaluate its effect in a prediabetic murine model by oral administration. A semi-pilot scale extraction was established and serjanic acid purification was followed using direct MALDI-TOF analysis. A diet induced obesity mouse model was used to determine the impact of serjanic acid over selected immunometabolic markers. Mice treated with serjanic acid showed decreased levels of cholesterol and triacylglycerols, increased blood insulin levels, decreased fasting blood glucose and improved glucose tolerance, and insulin sensitivity. At transcriptional level, the reduction of inflammation markers related to adipocyte differentiation is reported.

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Section: Biological Activity Assessmentsupporting

confidence: 91%

Section: Biological Activity Assessmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

et al. 2020

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“…38 Therefore, pharmacological research on the mixture of OA and UA was conducted by numerous researchers. [39][40][41] The combination of OA and UA has been widely used in clinical practices. 42 pH-zone-rening countercurrent chromatography provided a rapid and simple method for the enrichment of a mixture of OA and UA, which might be useful for the clinical use of OA and UA.…”

Section: Selection Of Solvent Systems For Ph-zone-rening Countercurrmentioning

confidence: 99%

Preparative separation of structural isomeric pentacyclic triterpene oleanolic acid and ursolic acid from natural products by pH-zone-refining countercurrent chromatography

Wang

Zhao

et al. 2019

RSC Adv.

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“…In addition, TLR7/8 agonists not only activate antigen-presenting cells (APCs) but also promote the activation of T cells and natural killer cells (NK) (47,48). Activation of TLR-7 results in the involvement of MyD88, MAL, IRAKs, and IKKα, which promote activation of IRF and production of type 1 IFNs and pro-in ammatory cytokines (49)(50)(51)(52). Because most tumor cells express high levels of tumorspeci c antigens, TLR7 agonists have attracted widespread interest in tumor immunotherapy (53).…”

Section: Introductionmentioning

confidence: 99%

A combined Globo H-OCT4-T7 immunotherapy restrains colorectal tumor growth

Xu¹,

Liu²,

Liu³

et al. 2021

Preprint

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Background: Malignant tumors express multiple common markers that might constitute targets for tumor immunotherapy. Methods: Here we performed antigen epitope screening and molecular docking to design a new combination vaccine based on covalently linking Globo H and Oct 4 with a TLR7 agonist. We mainly use flow cytometry to analyze the changes in lymphocyte differentiation after the vaccine is applied. Use Elisa technology to detect the antibodies produced by the vaccine and the changes in lymphokines. In order to supplement the immunological data, RNA sequencing was performed on untreated and vaccine-treated tumors to define the interference of the vaccine on the genome-wide gene expression profile.Results: Our results demonstrated that the Globo H-OCT4-T7 conjugate vaccine effectively induced cellular uptake, maturation and antigen presentation of BMDCs. The combination vaccine enhanced cytokine production, CTLs activity elicited high levels of IgG. Importantly, the tumor immune microenvironment was significantly improved in tumor-bearing mice treated by Globo H-OCT4-T7 conjugate vaccine, demonstrated by increased the ratio of M1 to M2 TAMs, reduced number of Tregs in PBMCs and promoted the infiltration of tumor-specific IFN γ-producing CD8+ T cells. Prophylactic vaccination of Globo H-OCT4-T7 significantly reduced tumor growth by >80 % in both CT26 transplanted mice and in a human PDX mouse model when exposing mice to the combined vaccine compared to control. Our novel, combination vaccine is safe and effective at preventing tumor growth in mice. Conclusions: This combination vaccine is more effective than the single vaccine, and thus has the potential to prevent OCT4 and Globo H from expressing cancer.

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Immunometabolic regulation by triterpenes of Eucalyptus tereticornis in adipose tissue cell line models

Cited by 12 publications

References 20 publications

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

Preparative separation of structural isomeric pentacyclic triterpene oleanolic acid and ursolic acid from natural products by pH-zone-refining countercurrent chromatography

A combined Globo H-OCT4-T7 immunotherapy restrains colorectal tumor growth

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