Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells

Lee, Young Sun; Han, Ok Kyung; Park, Chan Woo; Suh, Seong Il; Shin, Sang Woo; Yang, Chae Ha; Jeon, Tae Won; Lee, Eun Sil; Kim, Kwang Joong; Kim, Seong Ho; Yoo, Wang Keun; Kim, Hyo Jung

doi:10.1016/s0378-8741(02)00298-2

Cited by 63 publications

(33 citation statements)

References 20 publications

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“…Their use dates back more than 2,000 years, and was recorded in Shen Nong's Materia Medica written during the Han dynasty (Mckenna et al 2002). Studies from both pharmacology and clinical practices have demonstrated that A. membranaceus possesses immunostimulant properties and exhibits immunomodulating and immunorestorative effects both in vitro and in vivo (Cho and Leung 2007), which has been widely used as a tonic to enhance the body's natural defense mechanisms and to treat various immune disorders (Lee et al 2003). Scutellaria baicalensis GEORGI is another popular traditional Chinese herb widely used for the treatment of various inflammatory diseases in Asia.…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii

et al. 2011

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Toxoplasma gondii is a parasite that infects animals and humans worldwide. The standard treatment for toxoplasmosis is limiting due to toxic adverse effects, thus there is a need to identify new drugs that are less toxic. Both Astragalus membranaceus and Scutellaria baicalensis GEORGI are popular traditional Chinese herbs widely used for the treatment of various inflammatory diseases in Asia, and we have previously demonstrated that water extracts of A. membranaceus (AmE) and S. baicalensis GEORGI (SbE) have good efficacy in controlling T. gondii replication in mouse models. This study was designed to further evaluate their effects against developing tachyzoites of the RH strain of T. gondii in HeLa cell cultures. AmE, SbE, and TMP-SMX (trimethoprim-sulfamethoxazole) were added into the wells containing both HeLa cells and replicating T. gondii of green fluorescent protein (GFP)-expressing RH tachyzoites. The proliferation and morphous of the tachyzoites were observed, the fluorescence intensity expressed as the fluorescence gray scale value was measured, and the living tachyzoites were counted at different culture times after treatment. The results showed that, compared to untreated controls, parasites treated with either AmE or SbE had significantly decreased intracellular replication at 72, 96, and 120 h after treatment (P < 0.01); while compared to either AmE- or SbE-treated groups, SMX-treated groups had even significantly decreased replication (only a few living parasites were detected) at the above times (P < 0.01). Our data demonstrated that both AmE and SbE had remarkable in vitro activities against T. gondii.

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Section: Introductionmentioning

confidence: 99%

In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii

et al. 2011

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“…Reports also state that AR astragalosides may inhibit liver fibrosis by reducing TNF-␣ and TGF-␤1 production, as well as by scavenging active oxidants in activated Kupffer cells (Gui et al, 2006). In immuno-suppressed mouse spleen cells and Raw 264.7 cells, AR has been shown to induce proliferation (Lee et al, 2003(Lee et al, , 2005 and to reverse not only cyclophosphamide-induced repression of NK cell activity (Jin et al, 1994) but also impaired cell-mediated immunity in burned mice (Liang et al, 1994). AR also appears to exert immunomodulatory effects by regulating the expression of cytokines such as IL-1␣, IL-1␤, IL-6, and inducible nitric oxide synthase (iNOS) as well as the production of nitric oxide (NO) (Lee et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk

Ryu

Kim

Chun

et al. 2008

Journal of Ethnopharmacology

149

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“…bioactivities, such as hypotensive (Hikino et al, 1976), inducing vasodilatation (Zhang et al, 2005), antioxidative (Shirataki et al, 1997), immunostimulating (Lee et al, 2003), antiviral (Anonymous, 2003), inducing cancer cell apoptosis (Cheng et al, 2004), reducing the capillary hyperpermeability and alleviating the dyskinesia caused by cerebral ischemia (Quan and Du, 1998), inhibiting cyclooxygenase-2 (Kim et al, 2001), promoting the motility of human spermatozoa (Liu et al, 2004), enhancing cardiovascular function, protecting the myocardium in diabetic nephropathy , antiaging (Wang et al, 2003), hepatoprotective effect (Zhu et al, 2001), inhibiting sterol biosynthesis (Sung, 1999), and antibacterial .…”

mentioning

confidence: 99%

Absorption and Metabolism of Astragali Radix Decoction: In Silico, in Vitro, and a Case Study in Vivo

Xu¹,

Zhang²,

Shuhua³

et al. 2006

Drug Metab Dispos

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ABSTRACT:To profile absorption of Astragali Radix decoction and identify its orally absorbable constituents and their metabolites, four complementary in silico, in vitro, and in vivo methods, i.e., a computational chemistry prediction method, a Caco-2 cell monolayer model experiment, an improved rat everted gut sac experiment, and a healthy human volunteer experiment, were used. According to the in silico computation result, 26 compounds of Astragali Radix could be regarded as orally available compounds, including 12 flavonoids. In the in vitro and in vivo experiments, 21 compounds were tentatively identified by high-performance liquid chromatography-diode array detection-electrospray ion trap tandem mass spectrometry data, which involved calycosin, formononetin, (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan, 7,2-dihydroxy-3,4-dimethoxyisoflavan, calycosin We found that besides isoflavones, pterocarpans and isoflavans also could be metabolized by the intestine during absorption, and the major metabolites were glucuronides. In conclusion, the present study demonstrated that the flavonoids in Astragali Radix decoction, including isoflavones, pterocarpans, and isoflavans, could be absorbed and metabolized by the intestine. These absorbable compounds, which were reported to have various bioactivities related to the curative effects of Astragali Radix decoction, could be regarded as an important component of the effective constituents of Astragali Radix decoction.

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Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells

Cited by 63 publications

References 20 publications

In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii

In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii

Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk

Absorption and Metabolism of Astragali Radix Decoction: In Silico, in Vitro, and a Case Study in Vivo

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