Impact of plasma chemistry versus titanium surface topography on osteoblast orientation

Rebl, Henrike; Finke, Birgit; Lange, Regina; Weltmann, Klaus‐Dieter; Nebe, J. Barbara

doi:10.1016/j.actbio.2012.06.015

Cited by 38 publications

(55 citation statements)

References 48 publications

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“…These control results are in accordance with previous in vitro studies on Ti (Finke et al, 2011a;Finke et al, 2007;Nebe et al, 2007a;Rebl et al, 2012;Rebl et al, 2010) and other materials, such as electrospun polylactide non- …”

Section: F Kunz Et Alsupporting

confidence: 82%

“…Ti disks of technical purity (cp, grade 2; ThyssenKrupp, Essen, Germany), 11 mm in diameter and 1 mm in height with a mechanically polished surface on the upper side (graining 4,000, R a = 0.05 µm) (Rebl et al, 2012) were obtained from DOT GmbH (Rostock, Germany) and used for the chemical functionalisation, or as reference specimens.…”

Section: Titanium Discsmentioning

confidence: 99%

“…For this purpose, we used the F-actin disrupting agent cytochalasin D (Parreno et al, 2008) to artificially impair the adhesion-related cell functions. Recently, we recognised that titanium (Ti) surfaces functionalised with plasma-polymerised allylamine (PPAAm) have a strong promoting effect on physiologically normal cells in respect to morphology and cell attachment (Finke et al, 2007;Rebl et al, 2012;Rebl et al, 2010). Furthermore, our new results in vivo confirmed these findings -the bone-to-implant contact zone (histomorphometry) in the medial tibia of rats was decisively increased after six weeks if the titanium alloy (Ti6Al4V) implant was covered with PPAAm or an ethylenediamine (Gabler et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Kunz

Rebl²,

Quade³

et al. 2015

eCM

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Bone diseases such as osteoporosis, osteoarthritis and rheumatoid arthritis, impinge on the performance of orthopaedic implants by impairing bone regeneration. For this reason, the development of effective surface modifications supporting the ingrowth of implants in morbid bone tissue is essential. Our study is designed to elucidate if cells with restricted cell-function limiting adhesion processes benefit from plasma polymer deposition on titanium. We used the actin filament disrupting agent cytochalasin D (CD) as an experimental model for cells with impaired actin cytoskeleton. Indeed, the cell's capacity to adhere and spread was drastically reduced due to shortened actin filaments and vinculin contacts that were smaller. The coating of titanium with a positively charged nanolayer of plasma polymerised allylamine (PPAAm) abrogated these disadvantages in cell adhesion and the CD-treated osteoblasts were able to spread significantly. Interestingly, PPAAm increased spreading by causing enhanced vinculin number and contact length, but without significantly reorganising actin filaments. PPAAm with the monomer allylamine was deposited in a microwave-excited low-pressure plasma-processing reactor. Cell physiology was monitored by flow cytometry and confocal laser scanning microscopy, and the length and number of actin filaments was quantified by mathematical image processing. We showed that biomaterial surface modification with PPAAm could be beneficial even for osteoblasts with impaired cytoskeleton components. These insights into in vitro conditions may be used for the evaluation of future strategies to design implants for morbid bone tissue.

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Section: F Kunz Et Alsupporting

confidence: 82%

Section: Titanium Discsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Kunz

Rebl²,

Quade³

et al. 2015

eCM

View full text Add to dashboard Cite

show abstract

“…Topographical structures in combination with chemical grafting can manipulate the morphology of cells, the adhesive interaction with the substrate, including organization of focal contacts to control the biological responses of cells. Interestingly, cells lost their ability to align their actin fibers parallel to the grooves of the machined titanium if the surface was well-directed plasma-chemically modified [69] indicating that a specific surface chemistry is dominant vs. the topography.…”

Section: Cell Regulation By Chemical Characteristics Of a Materialsmentioning

confidence: 99%

“…Fabrication of 5 µm high pillars on a titanium surface was coated with an allylamine plasma polymer layer to generate a high density of positively charged amino groups [69]. On this surface, osteoblastic cells completely merged the pillar structure with strong adhesion both to the pillars and to the bottom of the titanium surface (Figure 2).…”

Section: Cell Regulation By Chemical Characteristics Of a Materialsmentioning

confidence: 99%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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Abstract:The interaction of cells with material surfaces is of fundamental relevance and contributes to the clinical success of implants. Cells sense their physico-chemical environment resulting in focal adhesion reorganization, spatio-temporal localization and activation of integrin dependent signalling proteins as well as phenotypic changes, e.g., of the actin cytoskeleton. The technology of designing instructive biomaterials involves chemical modifications by grafting of chemical groups, adhesion ligands and growth factors. Physical modifications of the materials are created by structuring the surfaces stochastically and geometrically and by modifications of the material stiffness. Insights into the mechanism at the interface that are involved in the regulation of cells such as stem cells, osteoblasts and chondrocytes by materials will advance the development of innovative biomaterials in regenerative medicine.

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Thin Film Plasma Functionalization of Polyethylene Terephthalate to Induce Bone-Like Hydroxyapatite Nanocrystals

Gashti

Hegemann

Stir

et al. 2013

Plasma Process. Polym.

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In this study polyethylene terephthalate (PET) were functionalized using Ar/O2 and NH3/C2H4 plasmas followed by incubation in simulated body fluids (SBF). Hydroxyapatite (HAp) formed on sheets was investigated with the aid of infrared spectroscopy (IR), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and X‐ray diffraction (XRD). IR spectra showed that plasma pre‐treatments increased the reactivity of PET toward the SBF by generation of polar domains. SEM images indicated that there is a thin and uniform porous layer of HAp with needle‐like nanocrystals covering the surface of plasma treated sheets. Amorphous HAp films closely resembled bone compositions which may suggest Ar/O2 and NH3/C2H4 plasmas as potentially useful tools for bone tissue regeneration procedures.

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Impact of plasma chemistry versus titanium surface topography on osteoblast orientation

Cited by 38 publications

References 48 publications

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Cell-material interaction

Thin Film Plasma Functionalization of Polyethylene Terephthalate to Induce Bone-Like Hydroxyapatite Nanocrystals

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