Implementation Validation Performed in Rwanda To Determine Whether the INNO-LiPA Rif.TB Line Probe Assay Can Be Used for Detection of Multidrug-Resistant
            <i>Mycobacterium tuberculosis</i>
            in Low-Resource Countries

Quezada, C.M.; Kamanzi, Eliane; Mukamutara, Julienne; Rijk, Pim de; Rigouts, Leen; Portaels, Françoise; Amor, Yanis Ben

doi:10.1128/jcm.00590-07

Cited by 18 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various genotypic molecular methods, especially nucleic acid–amplification technologies, arose in recent years(García de Viedma, 2003 ; Cuevas-Córdoba and Zenteno-Cuevas, 2010 ; Abebe et al, 2011 ), which included conventional sequencing (Martin et al, 2006 ), pyrosequencing (Jureen et al, 2006 ), high-resolution thermal melt analysis (Hoek et al, 2008 ; Ramirez et al, 2010 ), denaturing gradient gel electrophoresis (DGGE) (McCammon et al, 2005 ), solid-phase hybridization-line probe assays, INNO-LiPA Rif (Quezada et al, 2007 ). TB assay (Innogenetics, Belgium), GenoType MTBDR Plus assay (HainLifescience, Nehren, Germany) (Brossier et al, 2009 ), molecular beacon-based automated platform Xpert MTB/RIF (Boehme et al, 2010 ), and digital PCR (Pholwat et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis

Peng

Cui

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Background: Failure to early detect multidrug-resistant tuberculosis (MDR-TB) results in treatment failure and poor clinical outcomes, and highlights the need to rapidly detect resistance to rifampicin (RIF) and isoniazid (INH).Methods: In Multi-Fluorescence quantitative Real-Time PCR (MF-qRT-PCR) assay, 10 probes labeled with four kinds of fluorophores were designed to detect the mutations in regions of rpoB, katG, mabA-inhA, oxyR-ahpC, and rrs.The efficiency of MF-qRT-PCR assay was tested using 261 bacterial isolates and 33 clinical sputum specimens. Among these samples, 227 Mycobacterium tuberculosis isolates were analyzed using drug susceptibility testing (DST), DNA sequencing and MF-qRT-PCR assay.Results: Compared with DST, MF-qRT-PCR sensitivity and specificity for RIF-resistance were 94.6 and 100%, respectively. And the detection sensitivity and specificity for INH-resistance were 85.9 and 95.3%, respectively. Compared with DNA sequencing, the sensitivity and specificity of our assay were 97.2 and 100% for RIF-resistance and 97.9 and 96.4% for INH-resistance. Compared with Phenotypic strain identification, MF-qRT-PCR can distinguish 227 M. tuberculosis complexes (MTC) from 34 Non-tuberculous mycobacteria (NTM) isolates with 100% accuracy rate.Conclusions: MF-qRT-PCR assay was an efficient, accurate, reliable, and easy-operated method for detection of RIF and INH-resistance, and distinction of MTC and NTM of clinical isolates.

show abstract

Section: Discussionmentioning

confidence: 99%

Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis

Peng

Cui

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Culture of sputum on a solid agar medium could provide more reliable DST results but is extremely lengthy and significantly delays the initiation of adequate therapy [21] . Liquid-growth based methods and Nucleic Acid Amplifications techniques have demonstrated superior performance in many settings [22] , [23] , [24] , [25] , [26] .…”

Section: Discussionmentioning

confidence: 99%

Multi Drug Resistant Tuberculosis in Mosango, a Rural Area in the Democratic Republic of Congo

et al. 2014

View full text Add to dashboard Cite

Multidrug Resistant Tuberculosis (MDR-TB) is a serious threat which jeopardizes the worldwide efforts to control TB. The Democratic Republic of Congo (DRC) is one of 27 countries with a high burden of MDR-TB. Data on the magnitude, trends, and the distribution of MDR-TB in DRC are scanty. Kinshasa, the capital city of DRC which accounts for 20% of all TB cases nationwide, is notifying more than 80% of all MDR suspects. We report here a cluster of MDR-TB cases that was investigated in the Mosango health district, in the Bandundu south Province, DRC in 2008. Phenotypic Drug Sensitivity Testing and DNA sequencing were performed on 18 sputum specimens collected from 4 MDR-TB suspects and 5 household contacts. Sequencing data confirmed that the 4 suspects were indeed Rifampicin resistant cases. Sequencing of the rpoB gene showed that 3 cases (patients A, B and D) had a single mutation encoding a substitution to 526Tyr, 531Trp and 526Leu respectively. Patient C had a double mutation encoding a change to 531Leu and 633Leu. Two of the investigated cases died within 4 months of a second-line treatment course. Results highlight the need to enhance adequate laboratory services within the country for both clinical as well as surveillance purposes.

show abstract

“…The relative complexity of the PCR-based genotypic tests compared with the NRA and MODS may be a limitation to their use in resource-limited settings (RLS). Genotypic assays require well trained manpower though this is not as critical as previously believed [ 38 ]. Also required are equipment such as sonicators, thermocyclers, hybridization instruments, and a suitable laboratory infrastructure with unidirectional work flow to minimize contamination.…”

Section: Discussionmentioning

confidence: 99%

Direct susceptibility testing for multi drug resistant tuberculosis: A meta-analysis

et al. 2009

View full text Add to dashboard Cite

BackgroundOne of the challenges facing the tuberculosis (TB) control programmes in resource-limited settings is lack of rapid techniques for detection of drug resistant TB, particularly multi drug resistant tuberculosis (MDR TB). Results obtained with the conventional indirect susceptibility testing methods come too late to influence a timely decision on patient management. More rapid tests directly applied on sputum samples are needed. This study compared the sensitivity, specificity and time to results of four direct drug susceptibility testing tests with the conventional indirect testing for detection of resistance to rifampicin and isoniazid in M. tuberculosis. The four direct tests included two in-house phenotypic assays – Nitrate Reductase Assay (NRA) and Microscopic Observation Drug Susceptibility (MODS), and two commercially available tests – Genotype® MTBDR and Genotype® MTBDRplus (Hain Life Sciences, Nehren, Germany).MethodsA literature review and meta-analysis of study reports was performed. The Meta-Disc software was used to analyse the reports and tests for sensitivity, specificity, and area under the summary receiver operating characteristic (sROC) curves. Heterogeneity in accuracy estimates was tested with the Spearman correlation coefficient and Chi-square.ResultsEighteen direct DST reports were analysed: NRA – 4, MODS- 6, Genotype MTBDR® – 3 and Genotype® MTBDRplus – 5. The pooled sensitivity and specificity for detection of resistance to rifampicin were 99% and 100% with NRA, 96% and 96% with MODS, 99% and 98% with Genotype® MTBDR, and 99% and 99% with the new Genotype® MTBDRplus, respectively. For isoniazid it was 94% and 100% for NRA, 92% and 96% for MODS, 71% and 100% for Genotype® MTBDR, and 96% and 100% with the Genotype® MTBDRplus, respectively. The area under the summary receiver operating characteristic (sROC) curves was in ranges of 0.98 to 1.00 for all the four tests. Molecular tests were completed in 1 – 2 days and also the phenotypic assays were much more rapid than conventional testing.ConclusionDirect testing of rifampicin and isoniazid resistance in M. tuberculosis was found to be highly sensitive and specific, and allows prompt detection of MDR TB.

show abstract

Implementation Validation Performed in Rwanda To Determine Whether the INNO-LiPA Rif.TB Line Probe Assay Can Be Used for Detection of Multidrug-Resistant Mycobacterium tuberculosis in Low-Resource Countries

Cited by 18 publications

References 17 publications

Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis

Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis

Multi Drug Resistant Tuberculosis in Mosango, a Rural Area in the Democratic Republic of Congo

Direct susceptibility testing for multi drug resistant tuberculosis: A meta-analysis

Contact Info

Product

Resources

About