Improving the Antibacterial Property of Porcine Small Intestinal Submucosa by Nano-Silver Supplementation

Zhou, Hai Yang; Zhang, Jian; Rong, Yan; Wang, Qiang; Fan, Lie Ying; Zhang, Qi; Wang, Wei Jun; Hu, Zhiyan

doi:10.1097/sla.0b013e31821260f3

Cited by 30 publications

(13 citation statements)

References 30 publications

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“…However, one of the major concerns associated with SIS has been surgical site infection and wound contamination. A number of studies have therefore focused on improving the anti-bacterial property of SIS by incorporating molecules like silver nanoparticles [80] and bismuth thiol [81]. However, the first step to finding a potential intervention to this clinical problem should be the analysis of the complex host-SIS-bacteria interactome.…”

Section: Resultsmentioning

confidence: 99%

Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus

2012

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The use of biological scaffold materials for wound healing and tissue remodeling has profoundly impacted regenerative medicine and tissue engineering. The porcine-derived small intestinal submucosa (SIS) is a licensed bioscaffold material regularly used in wound and tissue repair, often in contaminated surgical fields. Complications and failures due to infection of this biomaterial have therefore been a major concern and challenge. SIS can be colonized and infected by wound-associated bacteria, particularly Staphylococcus aureus. In order to address this concern and develop novel intervention strategies, the immune microenvironment orchestrated by the combined action of S. aureus and SIS should be critically evaluated. Since the outcome of tissue remodeling is largely controlled by the local immune microenvironment, we assessed the innate immune profile in terms of cytokine/chemokine microenvironment and inflammasome-responsive genes. BALB/c mice were injected intra-peritoneally with heat-killed S. aureus in the presence or absence of SIS. Analyses of cytokines, chemokines and microarray profiling of inflammasome-related genes were done using peritoneal lavages collected 24 hours after injection. Results showed that unlike SIS, the S. aureus-SIS interactome was characterized by a Th1-biased immune profile with increased expressions of IFN-γ, IL-12 and decreased expressions of IL-4, IL-13, IL-33 and IL-6. Such modulation of the Th1/Th2 axis can greatly facilitate graft rejections. The S. aureus-SIS exposure also augmented the expressions of pro-inflammatory cytokines like IL-1β, Tnf-α, CD30L, Eotaxin and Fractalkine. This heightened inflammatory response caused by S. aureus contamination could enormously affect the biocompatibility of SIS. However, the mRNA expressions of many inflammasome-related genes like Nlrp3, Aim2, Card6 and Pycard were down-regulated by heat-killed S. aureus with or without SIS. In summary, our study explored the innate immune microenvironment induced by the combined exposure of SIS and S. aureus. These results have practical implications in developing strategies to contain infection and promote successful tissue repair.

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Section: Resultsmentioning

confidence: 99%

Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus

2012

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“…Multiple strategies are being tested for optimization of mesh–surface properties to reduce deletirious tissue–mesh reactions . Researchers have attempted to grow certain cells on medical scaffolds to improve biocompatibility and graft function .…”

Section: Discussionmentioning

confidence: 99%

“…Multiple strategies are being tested for optimization of mesh-surface properties to reduce deletirious tissue-mesh reactions. 9,10, [14][15][16] Researchers have attempted to grow certain cells on medical scaffolds to improve biocompatibility and graft function. [17][18][19][20][21][22][23] Several attempts have also been made to cell-coat surgical meshes for hernia repair.…”

Section: Discussionmentioning

confidence: 99%

Methodology of fibroblast and mesenchymal stem cell coating of surgical meshes: A pilot analysis

et al. 2013

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Coating of various synthetic, absorbable, and biologic meshes with mesenchymal stem cells (MSCs) and fibroblasts was analyzed qualitatively and quantitatively. Five hernia meshes-light weight monofilament polypropylene (Soft Mesh), polyester (Parietex-TET), polylactide composite (TIGR), heavy weight monofilament polypropylene (Marlex), and porcine dermal collagen (Strattice)-were coated with three cell lines: human dermal fibroblasts (HFs), rat kidney fibroblasts (NRKs), and rat MSCs. Cell densities were determined at different time points. Samples also underwent histology and transmission electron microscopic (TEM) analyses. It required HFs 3 weeks to cover the entire mesh, while only 2 weeks for NRKs and MSCs to do so. MSCs had no preference for any of the meshes and produced the highest cell densities on Parietex and TIGR. Substrate-preference accounted for the significantly lower fibroblast densities on TIGR than Parietex. Fibroblasts failed to coat Marlex. Strattice, which had the least surface area, generated comparable cell densities to Parietex. Both histology and TEM confirmed cell coating of mesh surface. Various prosthetics can be coated by certain cell strains. Both mesh composition and cell preference dramatically influence the coating process. This methodology provides foundation for novel avenues of modulation of host response to various modern synthetic and biologic meshes.

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“…The first two aspects, coagulation and infection prevention, apply to early skin‐wound healing . Growth factors promote subsequent germinal layer cell division and neovascularization, and various types of nano‐material such as gold and silver nanoparticles have been used to promote granulation tissue differentiation . However, several issues around skin‐wound healing are not fully resolved: (a) hemostatic and anti‐infective materials are often ineffective at promoting wound healing; (b) growth factors can induce hyperblastosis or tumorigenesis; (c) although remarkable effects of nano‐materials have been reported, gold or silver nanoparticles may remain in scar tissue for a long time .…”

Section: Introductionmentioning

confidence: 99%

Effect of the hyaluronic acid‐poloxamer hydrogel on skin‐wound healing: in vitro and in vivo studies

Li²,

Feng

et al. 2019

Anim Models and Exp Med

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Background Recent research into skin injury and wound healing has focused mainly on post‐trauma hemostasis, infection prevention, dermal regeneration and angiogenesis. However, less attention has been paid to air permeability and moisture loss prevention which also play important roles in injury healing. Methods In the present work, we prepared a hyaluronic acid‐poloxamer ( HA ‐ POL ) hydrogel and tested the therapeutic effect of the hydrogel on skin‐wound healing. Results The HA ‐ POL hydrogel transformed from sol to gel at 30°C, close to body temperature, and had stable moisturizing properties. HA ‐ POL hydrogel promoted skin‐wound healing and increased protein accumulation in the wound area. HA ‐ POL hydrogel allowed greater air permeability than Band‐aid, a typical wound covering. Results from transwell assays showed that the HA ‐ POL hydrogel effectively isolated skin‐wounds from bacterial invasion. Conclusion This work demonstrates the advantages of using HA ‐ POL gel materials in the treatment of cutaneous wounds.

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Improving the Antibacterial Property of Porcine Small Intestinal Submucosa by Nano-Silver Supplementation

Cited by 30 publications

References 30 publications

Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus

Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus

Methodology of fibroblast and mesenchymal stem cell coating of surgical meshes: A pilot analysis

Effect of the hyaluronic acid‐poloxamer hydrogel on skin‐wound healing: in vitro and in vivo studies

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