In silico study of fucoxanthin as a tumor cytotoxic agent

Januar, Hedi Indra; Dewi, Ariyanti Suhita; Marraskuranto, Endar; Wikanta, Thamrin

doi:10.4103/0975-7406.92733

Cited by 23 publications

(17 citation statements)

References 24 publications

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“…; Januar et al. ; Wijesinghe and Jeon ). The present study was therefore carried out to estimate the antioxidant and antiproliferative activities of methanolic (M), chloroform (C), ethyl acetate (E) and aqueous (A) extracts of four seaweeds belonging to the phyla: Ochrophyta (brown) – Turbinaria ornata and Rhodophyta – (red) – Kappaphycus alvarezii, Acanthophora spicifera and Gracilaria corticata , collected from the Mandapam coast of Tamil Nadu, India.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Extracts of Selected Red and Brown Seaweeds from the Mandapam Coast of Tamil Nadu

Murugan

Iyer

2013

Journal of Food Biochemistry

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Antioxidant and antiproliferative activities were examined for methanol (M), chloroform (C), ethyl acetate (E) and aqueous (A) extracts of Turbinaria ornata, Kappaphycus alvarezii, Acanthophora spicifera and Gracilaria corticata, using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, beta-carotene bleaching inhibition, ferrous ion chelation, total reducing activity and resazurin-based growth inhibition (in A549, HCT-15, MG-63 and PC-3 cell lines) assays. Higher growth inhibition was observed in A549 and MG-63 cells with higher values of total flavonoid content, DPPH radical scavenging and total reducing activities, and in PC-3 cells with higher beta-carotene bleaching inhibition. Positive correlation (P < 0.05) was observed between total phenolic content and total reducing activity. Although all seaweeds have been examined for antioxidant and brine shrimp cytotoxic activities, and for some, in some cancer cell lines, this is the first report of growth inhibition in the cell lines chosen for this study that represent highly prevalent cancers. PRACTICAL APPLICATIONSAlthough Turbinaria ornata, Kappaphycus alvarezii, Acanthophora spicifera and Gracilaria corticata have already been examined for various activities, including antioxidant and brine shrimp cytotoxicity, this is the first report of antiproliferative activity in the cell lines chosen for this study that represent highly prevalent cancers. Besides this, seaweeds like K. alvarezii find widespread use as thickening and gelling agents and A. spicifera is consumed in many parts of the world as food and a folklore remedy, including the Gulf of Mannar and Fiji. We hope that this report of antioxidant and antiproliferative activities of these four seaweeds will promote interest in their cultivation to further study their mechanism of cytotoxicity against the cell lines in this study to facilitate drug discovery efforts, besides the existing interest in them as nutraceuticals as well as food and fodder additives, among other applications. bs_bs_banner Journal of Food Biochemistry

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Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Extracts of Selected Red and Brown Seaweeds from the Mandapam Coast of Tamil Nadu

Murugan

Iyer

2013

Journal of Food Biochemistry

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“…Transcriptional p53 induced apoptosis and cell cycle arrest in G 0 /G 1 phase on cancer cells. Fucoxanthin has been reported to regulate the p53 expression level by cell cycle arrest and apoptosis in several human cancer cell lines [27]. Thus, it is speculated that fucoxanthin might induce cell cycle arrest and apoptosis in human bladder cancer cells, providing a potential new candidate of therapy drugs for the treatment of human bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

Fucoxanthin induces growth arrest and apoptosis in human bladder cancer T24 cells by up-regulation of p21 and down-regulation of mortalin

Wang¹,

Yang²,

Liu³

et al. 2014

ABBS

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Fucoxanthin, a natural carotenoid, has been reported to have anti-cancer activity in human colon cancer cells, human prostate cancer cells, human leukemia cells, and human epithelial cervical cancer cells. This study was undertaken to evaluate the molecular mechanisms of fucoxanthin against human bladder cancer T24 cell line. MTT analysis results showed that 5 and 10 mM fucoxanthin inhibited the proliferation of T24 cells in a dose-and timedependent manner accompanied by the growth arrest at G 0 /G 1 phase of cell cycle, which is mediated by the up-regulation of p21, a cyclin-dependent kinase (CDK)-inhibitory protein and the down-regulation of CDK-2, CDK-4, cyclin D1, and cyclin E. In addition, 20 and 40 mM fucoxanthin induced apoptosis of T24 cells by the abrogation of mortalin -p53 complex and the reactivation of nuclear mutanttype p53, which also had tumor suppressor function as wild-type p53. All these results demonstrated that the anticancer activity of fucoxanthin on T24 cells was associated with cell cycle arrest at G 0 /G 1 phase by up-regulation of p21 at low doses and apoptosis via decrease in the expression level of mortalin, which is a stress regulator and a member of heat shock protein 70, followed by up-regulation of cleaved caspase-3 at high doses.

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“…Afolayan, Bolton, Lategan, Smith & Beukes (2008) juga menduga ada hubungan antara ikatan alenik dengan sif at antiplasmodial pada fukosantin. Hasil analisis secara in silico memperlihatkan bahwa gugus karboksil pada fukosantin membentuk ikatan hidrogen dengan tubulin pada sel yang berakibat pada depolimerasasi mikrotubulin dan penghambatan siklus sel (Januar, Dewi,Marraskuranto & Wikanta, 2012 …”

Section: Uji Sitotoksisitasunclassified

Isolasi Fukosantin dari Rumput Laut Coklat Padina australis dan Sitotoksisitasnya terhadap Sel MCF7 dan Sel Vero

Nursid

Noviendri

Rahayu

et al. 2017

JPBKP

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ABSTRAKFukosantin merupakan karotenoid laut yang banyak terdapat pada rumput laut coklat. Fukosantin memiliki potensi yang menjanjikan untuk diaplikasikan pada bidang kesehatan. Padina australis merupakan salah satu rumput laut coklat yang banyak mengandung fukosantin. Penelitian ini bertujuan untuk mengisolasi fukosantin dari rumput laut P. australis dan mengetahui sitotoksisitasnya terhadap sel lestari kanker MCF7 dan sel normal Vero. Isolasi fukosantin dilakukan dengan menggunakan kromatografi kolom SiO 2 . Identifikasi fukosantin dilakukan dengan menggunakan liquid chromatography ion trap time of flight mass spectrofotometer (LC-IT-ToF-MS) berdasarkan profil serapan UV dan berat molekul. Uj i sitotoksik dilakukan dengan menggunakan uji (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (uji MTT). Fukosantin berhasil diisolasi dari ekstrak kasar metanol. Fukosantin terkonfirmasi pada panjang gelombang maksimum (maks) 447 nm pada spektrum UV. Puncak monoisotopik ion molekul fukosantin terdeteksi pada nilai m/z 659,3612 [M+H]. Hasil uji MTT memperlihatkan bahwa fukosantin dari P. australis memiliki sitotoksisitas terhadap sel MCF7 dengan nilai IC 50 sebesar 34,7 µg/ml dan relatif tidak toksik terhadap sel normal Vero dengan nilai IC 50 sebesar 1071,6 µg/ml. KATA KUNCI: rumput laut coklat, Padina australis, fukosantin, sitotoksisitas ABSTRACT Fucoxanthin is marine carotenoid that present in brown algae. It has considerable potential and promising application in human health. Padina australis is one of brown algae containing fucoxanthin. The aims of this research was to isolate fucoxanthin from P. australis and to investigate cytotoxicity against MCF7 cancer cell and Vero normal cell line. Fucoxanthin isolation was performed by using SiO 2 column chromatography. Fucoxanthin identification was done by using liquid chromatography ion trap time of flight mass spectrofotometer (LC-IT-ToF-MS) based on UV absorbance profile and molecular weight. Cytotoxicity test was evaluated by using

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In silico study of fucoxanthin as a tumor cytotoxic agent

Cited by 23 publications

References 24 publications

Antioxidant and Antiproliferative Activities of Extracts of Selected Red and Brown Seaweeds from the Mandapam Coast of Tamil Nadu

Antioxidant and Antiproliferative Activities of Extracts of Selected Red and Brown Seaweeds from the Mandapam Coast of Tamil Nadu

Fucoxanthin induces growth arrest and apoptosis in human bladder cancer T24 cells by up-regulation of p21 and down-regulation of mortalin

Isolasi Fukosantin dari Rumput Laut Coklat Padina australis dan Sitotoksisitasnya terhadap Sel MCF7 dan Sel Vero

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