In vitro and in vivo evaluation of bioglass microspheres incorporated brushite cement for bone regeneration

Hasan, Lemon; Kim, Boram; Padalhin, Andrew R.; Faruq, Omar; Sultana, Tamanna; Lee, Byong-Taek

doi:10.1016/j.msec.2019.109775

Cited by 41 publications

(37 citation statements)

References 53 publications

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“…[23][24][25] It has been demonstrated that the incorporation of bioactive particles into cement formulations can induce the formation of apatite when immersed in simulated body fluid solution (SBF). [26][27][28] Since 1985, 45S5 (45% SiO 2 , 24.5% CaO, 24.5% Na 2 O, and 6% P 2 O 5 ) bioactive glass has been applied clinically owing to its bioactive, resorptive, and osteoinductive characteristics. 29,30 Additionally, antimicrobial properties and the ability to stimulate dentin remineralization are suitable characteristics of a biomaterial for dental application.…”

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confidence: 99%

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

Assad

Batista

Anami³

et al. 2020

Int J of Appl Glass Sci

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Owing to their adequate adhesive, esthetic, and mechanical properties resin-based cement has been widely used in dental practice to bond metallic, ceramic, or polymeric indirect restorations (crowns, veneers, inlays, onlays, or fixed partial dentures) in mineralized dental tissues. 1-3 Compared to conventional types of cement, like zinc phosphate and glass ionomer, resin-based cement has improved adhesive and mechanical properties and it is less soluble. 2-4 However, because of their potential cytotoxic effect on pulp-dentine complex 5-7 and the possible occurrence of postoperative hypersensitivity owing to the absence of hermetical sealing on the tooth/cement interface provoked by a

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mentioning

confidence: 99%

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

Assad

Batista

Anami³

et al. 2020

Int J of Appl Glass Sci

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“…The most important criteria for designing a scaffold are that cells must interact with the scaffold and function properly (Hasan et al, ). The scaffold should promote cell attachment on its surface and subsequently, proliferation, and differentiation.…”

Section: Discussionmentioning

confidence: 99%

A biphasic calcium phosphate ceramic scaffold loaded with oxidized cellulose nanofiber–gelatin hydrogel with immobilized simvastatin drug for osteogenic differentiation

et al. 2019

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A novel bone scaffold containing bioceramic and biopolymer materials with an osteoinductive simvastatin molecule was developed to enhance bone regeneration. An oxidized cellulose nanofiber (OCNF)–Gelatin (Gel) hydrogel was loaded into a biphasic calcium phosphate (BCP) ceramic in which simvastatin was entrapped, resulting in a scaffold with both osteoconductive and osteoinductive properties. The fabricated scaffold showed interconnected porosity with micro‐ and macroporous orientation. After loading the OCNF–Gel (HG), the mechanical stability of the ceramic BCP scaffold was increased suitable for the application of hard tissue regeneration. Fourier‐transform infrared spectroscopy showed that simvastatin was successfully coated on the BCPHG scaffolds. OCNF, with its slower degradation, may contribute to the sustained release of drug from the scaffold. Initially simvastatin was released from the scaffold at high levels, then was constantly and gradually released for up to 4 weeks. Pre‐osteoblast MC3T3E1 cells were seeded on the scaffolds to investigate cell viability, morphology, and differentiation. The simvastatin‐loaded BCPHG‐S scaffolds showed better cell proliferation and spreading compared to other scaffolds. Immunostaining assays showed the expression of proteins responsible for osteogenic differentiation. Alkaline phosphatase and osteopontin were more highly expressed in the BCPHG‐S scaffold than in other scaffolds. These results suggest that simvastatin‐loaded BCPHG scaffolds provided physiological environments suitable for better osteogenic differentiation.

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“…A Skyscan Deskop Micro-CT 1172 (Aartselaar, Belgium) was used maintaining 60 kV voltage source 167 µA current. 19,20…”

Section: Methodsmentioning

confidence: 99%

“…A Skyscan Deskop Micro-CT 1172 (Aartselaar, Belgium) was used maintaining 60 kV voltage source 167 mA current. 19,20 Measurement of tensile strength The 1 mm thick 5 Â 20 mm (width Â length) membrane cut from circumferential direction of electrospun membrane samples and 2 Â 20 mm (diameter Â length) tubular vessels were mechanically examined using paper frame according to the previous study with slight modification (Universal testing machine, R&B Unitech TM, Korea). 10 In brief, the samples in frame were mounted onto the hook of the machine according to Figure 3(e).…”

Section: Physico-chemical Characterization Of Small-diameter Vascularmentioning

confidence: 99%

In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft

et al. 2020

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During development of functional tissue engineered vascular graft, ensuring endothelialization at inner layer and mechanically strong outer layer is considered as challenging. The study objective was to fabricate a multilayered artificial blood vessel by dual syringe electrospinning technique. The presented graft was composed of polycaprolactone-methoxy polyethylene glycol which acts as an inner layer whereas Poly [4,4'-methylenebis(phenyl isocyanate)-alt-1,4butanediol/di(propylene glycol)/polycaprolactone] (PUPCL) (12% w/v) was incorporated as the outer layer. To find out the optimum inner layer, different polycaprolactone-methoxy polyethylene glycol electrospun membranes varying methoxy polyethylene glycol content (0, 5, 10, 20, and 30% w/v) were prepared and evaluated. The characterizations revealed that blending of methoxy polyethylene glycol with polycaprolactone improved hydrophilicity and antithrombogenic properties compared to polycaprolactone or polyurethane polycaprolactone membrane. During in vitro assessment, the optimized polycaprolactone-20% methoxy polyethylene glycol membrane reinforced a greater differentiation of rat bone marrow stem cell into endothelial cells at both mRNA and protein levels by significantly inducing endothelial markers (CD31, Flk-1, and von Willebrand factor) expression. The polyurethane polycaprolactone membrane showed higher mechanical strength than polycaprolactone-methoxy polyethylene glycol while multilayered polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone vascular graft exhibited better result compared to single form. Therefore, the strong and bioactive polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone small diameter graft (<2 mm) prepared by simple fabrication process could be a promising preliminary platform for vascular tissue engineering application studies.

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In vitro and in vivo evaluation of bioglass microspheres incorporated brushite cement for bone regeneration

Cited by 41 publications

References 53 publications

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

Effect of hydroxyapatite and 45S5 bioactive glass addition on a dental adhesive resin cement

A biphasic calcium phosphate ceramic scaffold loaded with oxidized cellulose nanofiber–gelatin hydrogel with immobilized simvastatin drug for osteogenic differentiation

In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft

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