In vitro anti-aging activities of ginkgo biloba leaf extract and its chemical constituents

Abstract

To determine in vitro anti-aging activity and identify the active chemical constituents in Ginkgo leaf extracts. The antioxidant properties of Ginkgo biloba leaves extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging and 2,2'-azino-bis (3-ethylbenzothiazoline)-6 sulfonic acid radical (ABTS) scavenging assays. The inhibitory effects of extracts and chemical constituents on reactive oxygen species, matrix metalloproteinase-1 (MMP-1) and collagen tpye O level were tested using human dermal fibroblasts (HDFs). Reverse-phase liquid chromatography (RPLC) and ultra-performance liquid chromatography tandem mass spectroscopy (UPLC/MS/MS) were used to quantitatively analyze flavonoids and terpene trilactones in the extraction. The established quantitative analysis method was validated according to the regulatory guidelines. The extraction and compounds kaempferol 3-D-β-D-glucopyranoside, isorhamnetin-3-D-glucoside, myricetin, ginkgolide A and bilobalide could have potential anti-aging activities of G. biloba. The results suggest that G. biloba could be used as anti-aging products and as a cosmetic and medicinal raw material.

The Most Potent Natural Pharmaceuticals, Cosmetics, and Food Ingredients Isolated from Plants with Deep Eutectic Solvents

“…Many of them have pharmacological activitiesanticancer, antimicrobial, and anti-inflammatory. , Because of their diversity and interesting properties, they have become important ingredients in food, cosmetics, and pharmaceuticals. Their chemical synthesis is usually unprofitable or unsustainable, so efficient extraction from natural sources seems to be a very important direction in research and industrial development. − Table presents a few examples of terpenes and terpenoids, with their activities and possible applications. ,− …”

Ginkgo biloba: A Treasure of Functional Phytochemicals with Multimedicinal Applications

“…Flavonoids. Liquid chromatography-mass spectrometry (LC-MS) has been used to identify and separate several flavonoids found in G. biloba [21][22][23][24][25]. To date, 110 [26].…”

“…Ginkgo biloba extraction impaired ROS and MMP-1 degradation in HDFs because of elevated levels of flavonoids and lactones [25] Immunomodulatory effects…”

Assessment of components of Gingko biloba leaves collected from different regions of China that contribute to its antioxidant effects for improved quality monitoring

“…The chromatographic analysis was conducted under the following conditions: mobile phase, water with 0.2% phosphoric acid (A) and acetonitrile (B); flow rate, 0.5 mL/min; column temperature, 30 °C; injection volume, 5.0 μL; and detection wavelength, 270 nm. The elution gradient was as follows: 85-75% (A) at 0-25 min, 75-70% (A) at 25-40 min, 70-50% (A) at 40-45 min, 50-40% (A) at 45-55 min, 40-30% (A) at 55-70 min, and 30-30% (A) at 70-75 min (Wang et al, 2020).…”