In-vitro biomineralization and biocompatibility of friction stir additively manufactured AZ31B magnesium alloy-hydroxyapatite composites

Ho, Yee‐Hsien; Man, Kun; Joshi, Sameehan S.; Pantawane, Mangesh V.; Wu, Tso-Chang; Yang, Yong; Dahotre, Narendra B.

doi:10.1016/j.bioactmat.2020.06.009

Cited by 58 publications

(27 citation statements)

References 47 publications

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“…The second peak at 532.8 eV is attributed to the Ta–OH group, which is suggested as an effective functional group for inducing apatite nucleation. , Figure d indicates that the location of Ca 2p 1/2 and Ca 2p 3/2 is 347.4 and 351.0 eV, respectively, which is the fingerprint for Ca 2p in the standard HA . The intensity of the P 2p peak at 133.2 eV is assigned to the PO 4 3– groups . The C 1s peaks show a double peak configuration.…”

Section: Resultsmentioning

confidence: 92%

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Cai

Wang

et al. 2021

ACS Omega

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The effect of sintering atmosphere (air and Ar) and temperature (1100, 1200, 1300 °C) on the microstructure, mechanical properties, and bioactivity of hydroxyapatite/tantalum (HA/Ta) composites were systematically investigated by pressureless sintering of the mixture of HA and Ta powders. It shows that the sintering atmosphere greatly impacts the phase composition and microstructure of the HA/Ta composites. The higher diffusion of atoms promotes shrinkage and causes deeper reaction fusion between the HA matrix and Ta, which improved the interfacial binding of the HA/Ta composites. The refined grain structure and improved interfacial binding obtained within the Ar atmosphere compared to the air atmosphere benefit the mechanical properties. The maximum bending strength and shrinkage observed for the composites sintered at 1300 °C in the Ar atmosphere are 27.24 MPa and 6.65%, respectively. The cell counting kit-8 (CCK-8) method was used to investigate the in vitro cytocompatibility of HA/Ta composites. The results revealed that the HA/Ta composites sintered with different conditions have no cytotoxicity. The simulated body fluid (SBF) soaking results showed that all of the studied composites possess desirable bioactivity, as demonstrated by their ability to form calcium-deficient carbonate apatite layer on the surfaces. For composites sintered at 1300 °C, the surface apatite layer coverage of the composites obtained in the Ar atmosphere was increased by 139.7% than the ones obtained in air, which confirmed an enhanced bioactive mineralization ability. The results indicated that the HA/Ta composites sintered at 1300 °C in Ar possess desirable mechanical properties and bioactivity. This work opens up the new possibility for preparing HA-based composites and is of great value in biomedical applications.

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Section: Resultsmentioning

confidence: 92%

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Cai

Wang

et al. 2021

ACS Omega

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“…To design and prepare composites by matrix or surface modification can be an effective strategy to not only improve the corrosion resistance of the Mg matrix but also contribute to their osteogenic activity. 33 Our previous studies have proved that the combined application of nHAC and Mg-Ca can promote the repair of canine mandibular defects. 22,23 In this experiment, the CS with good biocompatibility and filmforming was added, to prepare the CS-nHAC/Mg-Ca composites with stable structure, with MAO-coated Mg-Ca alloys as the matrix materials and CS and nHAC as the reinforcement materials.…”

Section: Resultsmentioning

confidence: 97%

In vitro evaluation of freeze-drying chitosan-mineralized collagen/Mg–Ca alloy composites for osteogenesis

et al. 2022

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Magnesium (Mg) alloy with good mechanical properties and biodegradability is considered as one of the ideal bone repair materials. However, the rapid corrosion of Mg-based metals can pose harm to the function of an implant in clinical applications. In this study, micro-arc oxidation coating was prepared on the surface of the Mg–Ca matrix, then the chitosan and mineralized collagen (nano-hydroxyapatite/collagen; nHAC) were immobilized on the surface of the MAO/Mg–Ca matrix to construct the CS-nHAC/Mg–Ca composites of different component proportions (the ratio of CS to nHAC is 2:1, 1:1, and 1:2, respectively). The corrosion resistance, osteogenic activity, and angiogenic ability were extensively investigated. The results indicated that the CS-nHAC reinforcement materials can improve the corrosion resistance of the Mg matrix significantly and promote the proliferation and adhesion of mouse embryo osteoblast precursor cells (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs). In addition, the CS-nHAC/Mg–Ca composites can not only promote the alkaline phosphatase (ALP) activity and extracellular matrix mineralization of MC3T3-E1 cells but also enhance the migration motility and vascular endothelial growth factor (VEGF) expression of HUVECs. Meanwhile, the 2CS-1nHAC/Mg–Ca composite exhibited the optimum function characteristics compared with other samples. Therefore, considering the improvement of corrosion resistance and biocompatibility, the CS-nHAC/Mg–Ca composites are expected to be a promising orthopedic implant.

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“…This phenomenon was mainly attributed to the introduction of PDA coating and HA, which is in line with previous work reporting the positive role of PDA and HA dually functionalized biomaterials on surface wettability 37 – 39 . The enhancement of surface hydrophilicity was proven to enable a profound effect on initial inflammation and subsequent biological performance between the implant surface and host surrounding tissue, including cell adhesion, proliferation, differentiation, and the formation of new bone and blood vessels 28 , 38 . Collectively, the combination of a 3D macroporous structure, PDA-mediated immobilization of LYN and HA, nanoroughened surface topography, and improved hydrophilicity made HS@PDA-LYN/HA more likely to exhibit a beneficial effect on promoting bone regeneration both in vitro and in vivo.…”

Section: Resultsmentioning

confidence: 99%

Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

Zhang

et al. 2022

npj Regen Med

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Osteogenesis and osteoclastogenesis are closely associated during the bone regeneration process. The development of multifunctional bone repair scaffolds with dual therapeutic actions (pro-osteogenesis and anti-osteoclastogenesis) is still a challenging task for bone tissue engineering applications. Herein, through a facile surface coating process, mussel-inspired polydopamine (PDA) is adhered to the surface of a biocompatible porous scaffold followed by the immobilization of a small-molecule activator (LYN-1604 (LYN)) and the subsequent in situ coprecipitation of hydroxyapatite (HA) nanocrystals. PDA, acting as an intermediate bridge, can provide strong LYN immobilization and biomineralization ability, while LYN targets osteoclast precursor cells to inhibit osteoclastic differentiation and functional activity, which endows LYN/HA-coated hybrid scaffolds with robust anti-osteoclastogenesis ability. Due to the synergistic effects of the LYN and HA components, the obtained three-dimensional hybrid scaffolds exhibited the dual effects of osteoclastic inhibition and osteogenic stimulation, thereby promoting bone tissue repair. Systematic characterization experiments confirmed the successful fabrication of LYN/HA-coated hybrid scaffolds, which exhibited an interconnected porous structure with nanoroughened surface topography, favorable hydrophilicity, and improved mechanical properties, as well as the sustained sequential release of LYN and Ca ions. In vitro experiments demonstrated that LYN/HA-coated hybrid scaffolds possessed satisfactory cytocompatibility, effectively promoting cell adhesion, spreading, proliferation, alkaline phosphatase activity, matrix mineralization, and osteogenesis-related gene and protein secretion, as well as stimulating angiogenic differentiation of endothelial cells. In addition to osteogenesis, the engineered scaffolds also significantly reduced osteoclastogenesis, such as tartrate-resistant acid phosphatase activity, F-actin ring staining, and osteoclastogenesis-related gene and protein secretion. More importantly, in a rat calvarial defect model, the newly developed hybrid scaffolds significantly promoted bone repair and regeneration. Microcomputed tomography, histological, and immunohistochemical analyses all revealed that the LYN/HA-coated hybrid scaffolds possessed not only reliable biosafety but also excellent osteogenesis-inducing and osteoclastogenesis-inhibiting effects, resulting in faster and higher-quality bone tissue regeneration. Taken together, this study offers a powerful and promising strategy to construct multifunctional nanocomposite scaffolds by promoting osteo/angiogenesis and suppressing osteoclastogenesis to accelerate bone regeneration.

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In-vitro biomineralization and biocompatibility of friction stir additively manufactured AZ31B magnesium alloy-hydroxyapatite composites

Cited by 58 publications

References 47 publications

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

In vitro evaluation of freeze-drying chitosan-mineralized collagen/Mg–Ca alloy composites for osteogenesis

Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

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