In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Bharti, Dinesh; Jang, Si Jung; Lee, Sang-Yun; Lee, Sung‐Lim; Rho, Gyu‐Jin

doi:10.3390/cells9030557

Cited by 21 publications

(14 citation statements)

References 117 publications

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“…However, the studies on the molecular bases of mammalian meiosis have been hampered by some intrinsic obstacles. In the first place, the lack of reliable and robust in vitro culture systems of mammalian meiotic cells ( Handel et al, 2014 ; Sun et al, 2014 ; Komeya et al, 2018 ; Hayashi, 2019 ; Bharti et al, 2020 ) is an important drawback that raises the need to work with in vivo models. Besides, in females the main meiotic events take place during the embryonic phase, and the number of oocytes is scarce, which hinders molecular analyses (e.g., Hernández-López et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics of Meiosis in the Male Mouse

Geisinger

Rodríguez-Casuriaga

Benavente

2021

Front. Cell Dev. Biol.

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Molecular studies of meiosis in mammals have been long relegated due to some intrinsic obstacles, namely the impossibility to reproduce the process in vitro, and the difficulty to obtain highly pure isolated cells of the different meiotic stages. In the recent years, some technical advances, from the improvement of flow cytometry sorting protocols to single-cell RNAseq, are enabling to profile the transcriptome and its fluctuations along the meiotic process. In this mini-review we will outline the diverse methodological approaches that have been employed, and some of the main findings that have started to arise from these studies. As for practical reasons most studies have been carried out in males, and mostly using mouse as a model, our focus will be on murine male meiosis, although also including specific comments about humans. Particularly, we will center on the controversy about gene expression during early meiotic prophase; the widespread existing gap between transcription and translation in meiotic cells; the expression patterns and potential roles of meiotic long non-coding RNAs; and the visualization of meiotic sex chromosome inactivation from the RNAseq perspective.

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Section: Introductionmentioning

confidence: 99%

Transcriptomics of Meiosis in the Male Mouse

Geisinger

Rodríguez-Casuriaga

Benavente

2021

Front. Cell Dev. Biol.

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“…Therefore, many authors tried to appoint if murine or human ES cells have the ability for in vitro differentiating into primordial germ cells (PGCs) or oocyte-like cells (OLCs; Hayashi et al, 2012). Isolating and manipulating ovarian stem cells possess enormous medical, veterinary, and animal production applications (Bharti et al, 2020). Spontaneous oocyte-like and germ cell differentiation in the in vitro condition may cause spreading unique biotechnologies to explain infertility issues in humans.…”

mentioning

confidence: 99%

Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cells derived from human follicular fluid to oocyte‐like cell

Moghadam

Saki

Nikbakht

et al. 2020

Cell Biology International

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Follicular fluid (FF) is essential for developing ovarian follicles. Besides the oocytes, FF has abundant undifferentiated somatic cells containing stem cell properties, which are discarded in daily medical procedures. Earlier studies have shown that FF cells could differentiate into primordial germ cells via forming embryoid bodies, which produced oocyte‐like cells (OLC). This study aimed at isolating mesenchymal stem cells (MSC) from FF and evaluating the impacts of bone morphogenetic protein 15 (BMP15) on the differentiation of these cells into OLCs. Human FF‐derived cells were collected from 78 women in the assisted fertilization program and cultured in human recombinant BMP15 medium for 21 days. Real‐time polymerase chain reaction and immunocytochemistry staining characterized MSCs and OLCs. MSCs expressed germline stem cell (GSC) markers, such as OCT4 and Nanog. In the control group, after 15 days, OLCs were formed and expressed zona pellucida markers (ZP2 and ZP3), and reached 20–30 µm in diameter. Ten days after induction with BMP15, round cells developed, and the size of OLCs reached 115 µm. A decrease ranged from 0.04 to 4.5 in the expression of pluripotency and oocyte‐specific markers observed in the cells cultured in a BMP15‐supplemented medium. FF‐derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in promoting the differentiation of these cells, which may give an in vitro model to examine germ cell development.

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“…Generation and proposed therapeutic implications of iPSCs in infertility are presented in Figure 2 . Sources: [ 35 , 57 , 60 ].…”

Section: Implications For Stem Cells In Infertilitymentioning

confidence: 99%

Application of Stem Cell Therapy for Infertility

Saha

Roy

Corbitt

et al. 2021

Cells

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Infertility creates an immense impact on the psychosocial wellbeing of affected couples, leading to poor quality of life. Infertility is now considered to be a global health issue affecting approximately 15% of couples worldwide. It may arise from factors related to the male (30%), including varicocele, undescended testes, testicular cancer, and azoospermia; the female (30%), including premature ovarian failure and uterine disorders; or both partners (30%). With the recent advancement in assisted reproduction technology (ART), many affected couples (80%) could find a solution. However, a substantial number of couples cannot conceive even after ART. Stem cells are now increasingly being investigated as promising alternative therapeutics in translational research of regenerative medicine. Tremendous headway has been made to understand the biology and function of stem cells. Considering the minimum ethical concern and easily available abundant resources, extensive research is being conducted on induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSC) for their potential application in reproductive medicine, especially in cases of infertility resulting from azoospermia and premature ovarian insufficiency. However, most of these investigations have been carried out in animal models. Evolutionary divergence observed in pluripotency among animals and humans requires caution when extrapolating the data obtained from murine models to safely apply them to clinical applications in humans. Hence, more clinical trials based on larger populations need to be carried out to investigate the relevance of stem cell therapy, including its safety and efficacy, in translational infertility medicine.

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In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Cited by 21 publications

References 117 publications

Transcriptomics of Meiosis in the Male Mouse

Transcriptomics of Meiosis in the Male Mouse

Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cells derived from human follicular fluid to oocyte‐like cell

Application of Stem Cell Therapy for Infertility

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