In vitro proliferation of periodontal ligament-like tissue on extracted teeth

Iwata, Toshio; Mino, Chieko; Kawata, Toshitsugu

doi:10.1016/j.archoralbio.2016.11.015

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…Mechanical injury to bone can stimulate osteoblasts for bone formation that can interfere with the PDL regeneration since the cell types after injury determine the type of the newly formed tissue. Iwata et al [ 84 ] described a culturing method before transplantation by culturing the extracted tooth in a culture medium for 1 month for the proliferation of the remaining PDL cells that was covered half of the root surfaces. However, during this time, the preservation of the alveolar socket remains challenging for the subsequent tooth replantation.…”

Section: Challenges and Pathways Of Cell-based Pdl Regeneration For A...mentioning

confidence: 99%

A new direction in managing avulsed teeth: stem cell-based de novo PDL regeneration

et al. 2022

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Management of avulsed teeth after replantation often leads to an unfavorable outcome. Damage to the thin and vulnerable periodontal ligament is the key reason for failure. Cell- or stem cell-based regenerative medicine has emerged in the past two decades as a promising clinical treatment modality to improve treatment outcomes. This concept has also been tested for the management of avulsed teeth in animal models. This review focuses on the discussion of limitation of current management protocols for avulsed teeth, cell-based therapy for periodontal ligament (PDL) regeneration in small and large animals, the challenges of de novo regeneration of PDL on denuded root in the edentulous region using a mini-swine model, and establishing a prospective new clinical protocol to manage avulsed teeth based on the current progress of cell-based PDL regeneration studies.

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Section: Challenges and Pathways Of Cell-based Pdl Regeneration For A...mentioning

confidence: 99%

A new direction in managing avulsed teeth: stem cell-based de novo PDL regeneration

et al. 2022

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“…45 Saito et al have explored the effectiveness of teeth transplanted with periodontal ligaments in reducing tissue damage and promoting healing. 46 In periodontitis, granulation tissue is produced as a result of the host innate defensive response. 47 Cells in granulation tissue consist of fibroblasts 48 and inflammatory cells, 47,49 which can infiltrate tissues and differentiate into fibroblasts, macrophages, white blood cells, and random collagen fibers.…”

Section: Characteristics Of Inflammatory Tissuementioning

confidence: 99%

“…Periodontal treatments aim to achieve new attached connective tissue and periodontal ligaments to provide a blood supply and repair injury 45 . Saito et al have explored the effectiveness of teeth transplanted with periodontal ligaments in reducing tissue damage and promoting healing 46 . In periodontitis, granulation tissue is produced as a result of the host innate defensive response 47 .…”

Section: Inflammatory Microenvironmentmentioning

confidence: 99%

Application of biomaterials in periodontal tissue repair and reconstruction in the presence of inflammation under periodontitis through the foreign body response: Recent progress and perspectives

et al. 2021

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Periodontitis would cause dental tissue damage locally. Biomaterials substantially affect the surrounding immune microenvironment through treatment-oriented local inflammatory remodeling in dental periodontitis. This remodeling process is conducive to wound healing and periodontal tissue regeneration. Recent progress in understanding the foreign body response (FBR) and immune regulation, including cell heterogeneity, and cell-cell and cell-material interactions, has provided new insights into the design criteria for biomaterials applied in treatment of periodontitis. This review discusses recent progress and perspectives in the immune regulation effects of biomaterials to augment or reconstruct soft and hard tissue in an inflammatory microenvironment of periodontitis.

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“…Previously, we reported that when teeth are extracted without consideration of the remaining human periodontal ligament (HPDL), approximately 50% of the periodontal membrane area remained (10). Therefore, it is necessary to regenerate the HPDL of the root surface.…”

Section: Originalmentioning

confidence: 99%

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

2017

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Residual periodontal ligament (PDL)and cement mass on the roots of extracted teeth are factors that considerably affect tooth transplantation. Therefore, when normal extracted teeth are used for autologous transplantation, it is necessary to regenerate the PDL of the root surface. Here we describe a method to examine human PDL cell adhesion on sterilized root surfaces. Sample teeth were extracted during orthodontic treatment. PDL cells were obtained from healthy periodontal tissue explants from teeth extracted for orthodontic reasons. We developed a method for adhering PDL cells to sterile root surfaces using three-dimensional culture for 3 weeks. We evaluated the adhesion of human PDL cells to the sterilized root surfaces biochemically and histologically. The adherent PDL cells presented new projections on the sterile root surfaces. Therefore, PDL cells can adhere to sterile root surfaces.

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In vitro proliferation of periodontal ligament-like tissue on extracted teeth

Cited by 5 publications

References 36 publications

A new direction in managing avulsed teeth: stem cell-based de novo PDL regeneration

A new direction in managing avulsed teeth: stem cell-based de novo PDL regeneration

Application of biomaterials in periodontal tissue repair and reconstruction in the presence of inflammation under periodontitis through the foreign body response: Recent progress and perspectives

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

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