In vitro propagation of Rubus species

Jin, Wei; Gu, Yajia; Shen-Zhi, Zhen

doi:10.1016/0304-4238(92)90169-d

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…Medium factors were very effective in vitro propagation and regeneration studies (Wei et al 1992). Najaf-Abadi and Hamidoghli (2009) also observed the top condition for micropropagation of Thornless trailing blackberry (Rubus spp.…”

Section: Resultsmentioning

confidence: 99%

“…Research, 2022, Vol. 6 (3), 205-217 213 Seasonal elements highly influenced in vitro propagation and regeneration studies (Wei et al 1992). For this reason, in vitro propagation studies have increased in many plants in recent years, as well as in Rubus species (Andrade et al 2021;Raeva-Bogoslovskaya et al 2021;Muñoz-Concha et al 2021;Kefayati et al 2019;Borodulina et al 2019).…”

Section: Resultsmentioning

confidence: 99%

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Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Topçu¹

2022

IJIAAR

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Rubus spp. is a shrub-form plant known for its fruits called blackberries. Blackberries are plants with high commercial value, delicious taste, nice aroma, and high nutritional value. Turkey has wealthy genetic origins of Rubus species. Conventionally, the trading propagation of Rubus plants is done as vegetatively, utilizing truncation, rooting, or stratuming. However, these traditional methods are time-consuming and inefficient in virus-free plant production. Cloning of plant grown in the tissue culture also enables to obtain virus-free plants and to provide fast replicating high standard plants. Rubus obtained by micropropagation is used for the formation of commercial fruit plantations as well as source plant formation. In this work, the aim is the development of in vitro micropropagation process of the wild Rubus in the Trakya Region. Proliferation from axillary buds was made by adding BAP (6-Benzylaminopurine), NAA (Naphthalinacetic acid) and GA3 (Gibberellic acid) in various combinations and concentrations to the MS medium. Rooting was successfully realized with 83.3% rooted plants in 1 IBA medium. No roots were seen in 0 MS. The survival rate of plants transferred to ex vitro conditions was 100%.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Topçu¹

2022

IJIAAR

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“…They found that GA3 was essential for propagation in three of the four species used and had significant effects on the complex hormone system. It was known that GA3 could synergistically act with auxins (Wei, 1992).…”

Section: In a Study By Stoevska Et Al On Rubusmentioning

confidence: 99%

Development of an in Vitro Multiplication System for Rubus Loganobaccus L.

Saeideh¹,

Hossein²,

Mohammad³

et al. 2019

PTQ

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The Rubus loganobaccus L. variety is a hybrid produced from a cross between Rubus idaeus and Rubus ursinus, which is well-known for its exatic distinguished fruits. However, a commercial cost-effective multiplication method has not been introduced yet, which micropropagation as a reliable technique could be an efficient economic procedure to generate uniform plant materials. In this case, an experiment carried out using plant buds explants the most appropriate disinfection treatments found out to be 1 to 5 min in 0.1% mercuric chloride in which healthy buds grew with no fungal/bacterial contamination. The highest rooting percentage (96.66%) and the lowest rooting percentage (60%) were observed in concentrations of phytohormones in MS +BA( 1 mg∙L-1) IBA + (0.1 mg∙L-1) and MS + 2ip (1 mg∙L-1) + IBA (0.1 mg∙L-1), respectively. The maximum and the minimum average root length were 3 cm and 1 cm, respectively. In the acclimatization stage of the plant root, the maximum percentage of survived and fresh pots 96.43%, the maximum average number of branching 8.73, the minimum average number of branching 3.13 in treatments MS + BA( 1 mg∙L-1) + IBA(0.1 mg∙L-1) and hormonefree MS medium, respectively, the maximum branch length 5.22 cm, and the minimum branch length 1.71 cm in MS + 2ip (2 mg∙L-1) + IBA (0.1 mg∙L-1) and AN + BA (1 mg∙L-1) + IBA (0.1 mg∙L-1), respectively, were observed. Overall, this protocol comperhensively addresses the whole process of micropropagation and can be further be used in commercial propagation of Rubus loganobaccus and produce plant materials with superior advantages over conventional methods.

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“…The plant growth regulators used in blackberry tissue cultures include benzyladenine (BA) and indolebutyric acid (IBA) (Lazic and Ruzic, 2007). The rooting of microcuttings can either be performed in vitro, as is already widely used (Deng and Donnelly, 1993) or ex vitro during acclimatization, directly on the substrate (Jin et al, 1992;Augusto et al, 2006) or in float hydroculture (Clapa et al, 2013). Ex vitro rooting offers the advantage of reducing difficulties related to survival and development of plants cultivated in vitro (Augusto et al, 2006;Pelizza et al, 2013) and also reduces costs.…”

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confidence: 99%

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

Bueno

Biasi

Tofanelli

2018

Rev. Colomb. Cienc. Hortic.

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This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

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In vitro propagation of Rubus species

Cited by 6 publications

References 9 publications

Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Development of an in Vitro Multiplication System for Rubus Loganobaccus L.

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

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