In vitro PROPAGATION OF SOME CITRUS ROOTSTOCKS. 1- EFFECT OF EXPLANT SOURCE AND GROWING MEDIA GROWTH REGULATORS CONTENT.

El-Boray, M. S. S.; Mustafa, M. F. M.; Shalan, A. M.; Abed, A. A.

doi:10.21608/jpp.2015.49791

Cited by 1 publication

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“…Cytokinin's are plant growth regulators that promote cell division, break apical dominance, and stimulate the growth of adventitious shoots and axillary buds in plants [13]. [14] studied the effect of 1.5 mg l -1 BA or 1 mg l -1 Kinetin and the interaction between them on the proliferation of shoots from (shoot tips and epicotyl) for five Citrus rootstocks, Sour orange (Citrus aurantium, L.), Volkamer lemon (Citrus volkmeriana, Ten. As well as the highest number and length of roots were obtained in the same medium for all rootstocks.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Three Citrus Rootstocks by Tissue Culture

Ahmed

Bashi

Alwan

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The experiments were conducted in the Laboratory of Tissue culture/Department of Forestry/College of Agriculture and Forestry/the University of Mosul. To propagate three citrus rootstocks (Citrus aurantium L., Citrumelo swingle, and Cariso 35 (C35))In vitro. During the Initiation stage, the single nodes were planted on a solid MS medium supplemented with five concentrations of BA (0, 1, 2, 3, and 4 mg L-1). After four weeks, the best significant percentage of response for Cariso35 and Citrumelo swingle, while the highest value of BA effect was at 2 mg BA L-1(96.67%). The No. of shoots there are nonsignificant differences, While the best No. of the shoot (1.41) obtained at 2 mg BA L-1, The highest significant shoot length (2.14cm) was obtained at Cariso35 compared with other rootstocks, so that, 1mg BA L-1 was achieved (2.01 cm). At the multiplication stage, Citrumelo swingle achieved the highest significant value (2.68shoots), whereas adding 4 mg BA L-1 gave the best significant No. shoots (3.10). as soon as, the shoot length (1.03cm) was obtained for Cariso35, while control treatment achieved a significant increase in shoot length (1.30cm) which differ significantly from other treatments. The highest significant number of leaves was (11.50 leaves) for Citrumelo swingle, while BA at 2 mgL-1 was dominated value achieved at 11.00 leaves. In the rooting stages, the best rooting percentage was 60% for Cariso 35 cultured on MT solid medium, but a non-significant difference between all IBA concentrations. While the significant No. of roots was 3.28 obtained from Citrumelo swingle, and the concentration 1.5 and 2 mg IBA L-1 achieved the highest significant values (2.90 and 2.24 root) respectively. Citrumelo swingle and the 1.5 mg IBA L-1 gave the shortest significant duration to formed roots which were 21.56 and 18.60 days respectively.

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Section: Introductionmentioning

confidence: 99%