In vitro ruminal fermentation of tanniniferous tropical plants: Plant-specific tannin effects and counteracting efficiency of PEG

Tiemann, Tassilo T.; Avila, P.; Ramírez, G.; Lascano, Carlos E.; Kreuzer, Michael; Heß, Hans

doi:10.1016/j.anifeedsci.2007.12.009

Cited by 52 publications

(41 citation statements)

References 25 publications

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“…Getachew et al (2008a) observed higher faecal N as the tannic acid level on lucerne hay was increased. Tiemann et al (2008) studied the effect of using two tannin-rich shrub legume species as replacements for a herbaceous high-quality legume (Vigna unguiculata) on the nutritive value of a diet composed of tropical grasses (Brachiaria brizantha and V. unguiculata). They found that faecal N losses increased linearly relative to N intake with increasing proportion of CT-rich legumes in the diet, while the proportionate urinary N losses decreased for both CT-rich legume shrubs.…”

Section: N Utilisationmentioning

confidence: 99%

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Aufrere

Dudilieu

Andueza

et al. 2013

Animal

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The aim of this study was to investigate whether the use of sainfoin-based condensed tannins (CT) enhances feed value when given with tannin-free legumes (lucerne) to sheep. The experiments were conducted with fresh sainfoin and lucerne harvested at two stages (vegetative stage as compared with early flowering) in the first growth cycle. Fresh sainfoin and lucerne forages were combined in ratios of 100 : 0, 75 : 25, 25 : 75 and 0 : 100 (denoted S100, S75, S25 and S0, respectively). Voluntary intake, organic matter digestibility (OMD) and nitrogen (N) retention were measured in sheep fed the different sainfoin and lucerne mixtures. Loss of dry matter (DM) and N from polyester bags suspended in the rumen, abomasum and small intestine (SI) was also measured using rumen-fistulated sheep and intestinally fistulated sheep. The CT content in sainfoin (S100) decreased with increasing percentage of lucerne in the mixture (mean value from 58 g/kg DM for S100 to 18 g/kg DM for S25) and with growth stage (S100: 64 to 52 g/kg DM). OMD did not differ between different sainfoin/lucerne mixture ratios. Sainfoin and lucerne had an associative effect (significant quadratic contrast) on voluntary intake, N intake, total-tract N digestibility, N in faeces and urine (g/g N intake) and N retained (g/g N intake). Compared with lucerne mixtures (S0 and S25), high-sainfoin-content mixtures (S100 and S75) increased the in situ estimates of forage N escaping from the rumen (from 0.162, 0.188 for S0 and S25 to 0.257, 0.287 for S75 and S100) but decreased forage N intestinal digestibility (from 0.496, 0.446 for S0 and S25 to 0.469, 0.335 for S75 and S100). The amount of forage N disappearing from the bags in the SI (per g forage N) was the highest for high-sainfoin mixtures (from 0.082, 0.108 for S100 and S75 to 0.056, 0.058 for S25 and S0, P , 0.001). Rumen juice total N (tN) and ammonia N (NH3-N) values were the lowest in the high-sainfoin diet (mean tN 0.166 mg/g in S100 as compared with 0.514 mg/g in S0; mean NH3-N 0.104 mg/g in S100 as compared with 0.333 mg/g in S0, P , 0.001).Keywords: ruminants, in vivo, condensed tannins, sainfoin-lucerne mixtures. ImplicationsLucerne is favoured for its high yield and nutritional quality, but is seldom grazed in pure stands as it causes bloating. Condensed tannins (CT)-containing legumes do not cause bloating and form CT-protein complexes that are stable and insoluble at rumen pH. Several papers have focused on the effect of CT on the nutritive value of CT-containing forages. The aim of this study was to investigate whether the use of sainfoin CT enhances feed value when given with tannin-free legumes (lucerne). We tested the effects of different sainfoin/lucerne proportions on voluntary intake, in vivo digestibility and in situ ruminal and intestinal digestibility in sheep.

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Section: N Utilisationmentioning

confidence: 99%

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Aufrere

Dudilieu

Andueza

et al. 2013

Animal

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“…However, when feed supplementation is incorporated to improve ruminant production then employing additives that have an effect on greenhouse gas production would be beneficial for individual producers and the industry. A number of feed additives such as halogenated analogues [4], monensin [5] and a range of plant compounds such as tannins [6], saponins [7], essential oils [8,9], and various secondary metabolites [10] have been demonstrated to have potential in reducing greenhouse gas emission from livestock production systems, but in most part it's difficult to administer across large herds which are managed in extensive environments.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tropical Algae as Additives on Rumen <i>in Vitro</i> Gas Production and Fermentation Characteristics

Dubois¹,

Tomkins²,

Kinley³

et al. 2013

AJPS

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Algae have become an area of intensive research in many fields of study. Areas of application are becoming increasingly diverse with the advent of technologies particularly in the mass production of algae biomass. Algae contain complex bioactive compounds and these are gaining importance in emerging technologies with nutritional and environmental applications. In this study, a preliminary investigation evaluated 15 species of algae from the major categories of marine and fresh water algae for their potential as inclusions in ruminant diets for management of greenhouse gas emissions. It was hypothesized that algae would positively affect rumen fermentation and gas production while reducing methane production. The hypothesis was tested using an Ankom automated gas monitoring system and rumen fluid from Bos indicus steers fed tropical forage diets. The results were variable between algae species with some showing a significant reduction in total gas and methane production, with others increasing gas and fermentation. The red and brown algae stand out as having potential for greenhouse gas mitigation with the brown alga Cystoseira having the most prominent effect. The effects observed on fermentation may be manipulated through dosage management and beneficial effects could be potentially maximized by preparing combinations of algal supplements. It has been demonstrated in this study that algae have the potential to assist in rumen fermentation management for improved gas production, and greenhouse gas abatement.

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“…Ammonia concentration of trash fish silage protected with C. calothyrsus and L. leucocephala leaves in this research was 15.97 -20.85 mg/dL, sufficient for rumen microbe growth. Supplementing CT from different plant to protein enriched feed did not generally affect microbial protein synthesis in rumen (Bento et al, 2005;Tiemann et al, 2008b;Wischer et al, 2013), but slightly lowered rumen microbe population by increasing the level of CT (McSweeney et al, 2001). It was due to the lack of nutrient for rumen bacteria.…”

Section: Effects On In Vitro Ruminal Fermentation Product and Microbimentioning

confidence: 99%

“…Hess et al (2008) and Tiemann et al (2008b) reported that the increasing legume CT in fermentation grass subtrate in vitro reduce the activity of fibrolytic microbe, thereby decreasing acetic acid concentration and increased propionic acid proportion. This research result showed that CT of C. calothyrsus and L. leucocephala leaves did not optimally reduce fibrolytic rumen microbe as seen from the non-increasing propionic acid concentration, and no different acetic and propionic ratio.…”

Section: Effects On In Vitro Ruminal Fermentation Product and Microbimentioning

confidence: 99%

“…Local and hybrid L. leucocephala protect protein in rumen differently (Huang et al, 2010), so as with C. calothyrsus cultivar (Tiemann et al, 2008b). The difference is shown in equal CT concentration in purified C. calothyrsus and L. leucocephala, demonstrating different characteristic regarding in vitro ruminal nutrient degradation (Tiemann et al, 2008a;Cortes et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility

Rimbawanto

Yusiati

Baliarti

et al. 2015

anprod

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Abstract. Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4 and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased and increased total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.Key words: condensed tannin, Leucaena, Calliandra leaves, rumen fermentation, trash fish silage Abstrak. Dua tanaman sebagai sumber tanin kondensasi ditambahkan pada protein silase ikan rucah (SIR) untuk mengetahui pengaruhnya terhadap produk fermentasi dan sintesis protein mikrobia rumen, dan kecernaan secara in vitro. Penambahan tanin kondensasi pada protein silase ikan rucah pada proporsi kontrol (0), di bawah level optimum (2,0%), level optimum (4,0%), dan di atas level optimum (6,0% of g SIR) presipitasi protein bovine serum albumin (BSA) dengan tanin kondensasi L. leucocephala dan di bawah level optimum (1,5%), level optimum (3,0%), dan di atas level optimum (4,5% of g SIR) presipitasi protein BSA dengan tanin kondensasi C. calothyrsus. Pengaruh degradasi di dalam cairan rumen dan inkubasi dalam HCl-pepsin dievaluasi menggunakan metode in vitro dua tahap. Kadar tanin kondensasi L. leucocephala dan C. calothyrsus berturut-turut 4, 6% dengan presipitasi protein BSA 26.25, 31.77 g BSA/g tanin kondensasi. Penambahan tanin kondensasi pada silase ikan rucah, produk fermentasi rumen (NH3-N, C2, C3, and VFAs total) dan kecernaan (BK and BO) di dalam rumen menurun dan meningkatkan kecernaan PK total (dalam HCl-pepsin/paska rumen). Perbedaan tersebut dipengaruhi oleh sumber tanin kondensasi tanpa mempengaruhi sintesis protein mikrobia di dalam rumen. Penggunaan tanin kondensasi dari L. leucocephala lebih baik dalam meningkatkan kecernaan protein total SIR 70% dibanding C. calothyrsus 15%. Ini menunjukkan bahwa tanin kondensasi dari C. calothyrsus kurang ...

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In vitro ruminal fermentation of tanniniferous tropical plants: Plant-specific tannin effects and counteracting efficiency of PEG

Cited by 52 publications

References 25 publications

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Effect of Tropical Algae as Additives on Rumen <i>in Vitro</i> Gas Production and Fermentation Characteristics

Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility

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In vitro ruminal fermentation of tanniniferous tropical plants: Plant-specific tannin effects and counteracting efficiency of PEG

Cited by 52 publications

References 25 publications

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins

Effect of Tropical Algae as Additives on Rumen &lt;i&gt;in Vitro&lt;/i&gt; Gas Production and Fermentation Characteristics

Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility

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Product

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Effect of Tropical Algae as Additives on Rumen <i>in Vitro</i> Gas Production and Fermentation Characteristics