In vitro Shoot Multiplication from Nodal Explants of Coccinia grandis (L.) Voigt. and It’s Antidiabetic and Antioxidant Activity

Abstract

Coccinia grandis (L.) Voigt. (Cucurbitaceae) is an important vegetable with high food and medicinal value. In this plant major problem is seed germination, seed setting and less number of fruit settings. Plant tissue culture technology may help to overcome these problems to a great extent. Therefore, the present study has been designed to develop an efficient protocol for in vitro shoot multiplication, as well as to check its antidiabetic and antioxidant activity. The nodal segments used as explants for cultured. Murashige and Skoog's (MS) agar-gelled medium with different concentration and combination of BAP, KIN, NAA and NB 6 for shoots multiplication and callus. Multiple shoots was seen after 10 days of sub culturing on MS medium. Maximum shoot formed (16±1.5) on MS medium containing 0.5 mg LG 1 BAP combination with 0.2 mg LG 1 NB 6 after 15 days. Antidiabetic activity of C. grandis was also evaluated by using α-amylase inhibition assay and DNS assay. Methanolic extract of fruit, callus (BAP+NB 6 ) and different combination mixture of callus at 1.0 mg LG 1 concentration showed 54.29, 75.72 and 80.00% inhibition of α-amylase activity respectively. The result of agar diffusion amylase inhibition assay indicated that methanolic extract of callus show maximum inhibition (46.66%) compare to the fruit extract and fresh callus showed no inhibition. Antioxidant activity of C. grandis was evaluated by using DPPH assay method is based on the reduction of methanolic solution of coloured free radical DPPH by free radical scavenger. The DPPH radical scavenging activity and significantly decreased the formation of oxygen radicals generated in rat peritoneal macrophages plant extracts. The protocol described here is efficient with high number of shoot multiplication and can be employed for the large scale production and genetic manipulation for antidiabetic medicinal plant Coccinia grandis.

Isolation and extraction of gallic acid from Hylocereus undatus and a biochemical and histological study on laboratory Wistar albino rats with induced rheumatoid arthritis

“…Also, the biological and protective effect of the aqueous extract of dragon fruit (H. undatus) (kg/mg 500) showed a significant increase in its weight (3.36650 ± 332 g ), P<0.05 when compared with the positive control group (2.67603 ± 200.833g), and this may be because these doses of plant extract may effect on sensory perception, and then effect on appetite ( a, b, c, and d) refer to the significant differences at P<0.05; Different letters (a, b, c, and d) refer to the non-significant differences at P<0.05); FCA: Freund's Complete Adjuvant Table 1. Showing relative weights of the rats for the four groups al., 2020), and dragon fruits H. undatus extract is a good source of polyphenols that have antioxidant properties (Hernández and Salazar, 2012); And that the pulp and peel of this fruit have many chemical compounds that possess antimicrobial activity that can be used as a natural antioxidant (Patel and Ishnava, 2019), as well as containing many vitamins and minerals which have a significant effect on the body health (Hossain et al, 2021). The protective effect of Gallic acid showed a significant increase in its weight at the probability level (P<0.05) (3.51584 ± 284.1667 g) when compared with the positive control (2.67603 ± 200.833 g) of the body (Reckziegel et al, 2016) By influencing the two hormones responsible for feeling full and improving insulin resistance, leptin and adiponectin (Hsu and Yen, 2007).…”

Possibilities and Challenges of Dragon Fruit in Chapainawabganj Region of Bangladesh from the Growers Perspective

“…Dragon fruit is regarded as the "Powerhouse of Nutrition" among all fruits. Due to its low calorie, cholesterol-free, and antioxidant content, it reduces heart disease risk and keeps blood pressure stable (Patel & Ishnava, 2019). Betalains have the potential to decrease cancer cells and can fight oxidative stress.…”

Antidiabetic activity of Coccinia grandis (L.) Voigt: Bioactive constituents, mechanisms of action, and synergistic effects

“…The fruit extract of C. grandis obtained by various methods of extraction, i.e., decoction, alcohol maceration, water maceration, and Soxhlet extraction, showed inhibition of α-glucosidase activity (Alagar et al, 2014). The methanol extract of C. grandis fruits, callus, and different combination mixtures of callus at a concentration of 1.0 mg/l inhibited α-amylase enzymatic activity with inhibition percentages of 54.29%, 75.72%, and 80.00%, respectively (Patel and Ishnava, 2015). Pulbutr et al (2017) revealed that the aqueous extract of leaves and stems of C. grandis significantly inhibited α-amylase activity with IC 50 of 8.09 ± 0.72 and 8.06 ± 1.27 mg/ml, respectively.…”