In vitro Study of Collagen Coating of Titanium Implants for Initial Cell Attachment.

Nagai, Megumi; Hayakawa, Tohru; Fukatsu, Akira; Yamamoto, Masafumi; Fukumoto, Masahiko; Nagahama, Fumio; Mishima, Hiroyuki; Yoshinari, Masao; Nemoto, Kimiya; Kato, Takao

doi:10.4012/dmj.21.250

Cited by 76 publications

(46 citation statements)

References 20 publications

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“…Nagai et al [27] observed the formation of lamellipodia and filopodia in human gingival fibroblasts cultured on titanium after collagen coating. However, in the present study, fibronectin immobilization did not cause any significant change in cell morphologies.…”

Section: Discussionmentioning

confidence: 99%

Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

et al. 2006

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The aim of this study is to evaluate the influence of fibronectin immobilization on poly-L-lactic acid (PLA) films on the initial attachment of human gingival fibroblasts. Carboxylic acid groups are chemically introduced on the PLA films' surface by surface hydrolysis with 0.5 M NaOH. The contact angle of PLA surface with respect to double-distilled water decreases significantly after NaOH hydrolysis. X-ray photoelectron spectroscopy (XPS) also reveals significantly higher intensities of C C¼O /C C-O after NaOH hydrolysis. Fibronectin is immobilized onto the hydrolyzed PLA surface through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLA surface and the amino groups of fibronectin using water-soluble carbodiimide. XPS analysis shows that the fibronectin-immobilized PLA surface is enriched with nitrogen atoms. The immobilization of fibronectin significantly enhances the number of initially attached human gingival fibroblasts on the PLA surface. No obvious *Author to whom correspondence should be addressed. E-mail: m-nagai@mascat.nihon-u.ac.jp KEY WORDS: polylactic acid, fibronectin, fibroblast, initial cell attachment. JOURNAL OF BIOMATERIALS APPLICATIONS

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Section: Discussionmentioning

confidence: 99%

Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

et al. 2006

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“…Collagen or fibronectin immobilization was performed according to our previous report 13,17) . First, approximately 1 g of PLCL (PLA:PCL=75:25, LCL75:25 , BMG Inc, Kyoto, Japan) was dissolved in 20 ml chloroform.…”

Section: Fabrication Of Collagen or Fibronectin Immobilized Plcl Filmmentioning

confidence: 99%

MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

Fusé

Hayakawa

Hashizume-Takizawa

et al. 2015

J. Hard Tissue Biology.

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In the present study, collagen (Coll) or fibronectin (FN) was immobilized onto poly(lactic acid)/ -caprolactone (PLCL) copolymer surface and the attachment and proliferation of osteoblast-like cells on Coll or FN immobilized PLCL (Coll-PLCL, FN-PLCL) were evaluated. Coll or FN immobilized PLCL was prepared through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLCL (PLCL-COOH) surface and the amino groups of Coll or FN using water soluble carbodiimide. The contact angle of the PLCL-COOH, Coll-PLCL and FN-PLCL surfaces with respect to double distilled water significantly decreased compared with PLCL (p<0.05). Number of attached cells onto Coll-PLCL or FN-PLCL films was significantly greater than on PLCL films from at the time of 90 min to 7days (p<0.05). The morphological differences of attached osteoblast-like cells were observed between PLCL films and Coll-PLCL or FN-PLCL films at the early stage of cell assay. The cell attached on Coll-PLCL and FN-PLCL films appeared more flat than on PLCL films. Additionally, many actin filaments and stress fibers were observed on Coll-PLCL or FN-PLCL films during 3 and 7 days of cell assay. Actin filaments and stress fibers were few on PLCL films. These data demonstrated the effectiveness of collagen and fibronectin immobilization of PLCL for cell attachment and proliferation. This indicates that collagen or fibronectin immobilization of PLCL films was effective for biological activities of osteoblast-like cells.

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“…Thus, coating titanium surfaces with collagen may enhance cell activity or initial cell attachment around titanium implants, resulting in improved tissue responses. Nagai et al 11) reported that collagen coating enhanced the attachment of the human gingival fibroblasts in comparison with non-coating. Morikawa et al 12) immobilized collagen on titanium surface using gold deposition and production of a stable monomolecular layer with cysteine.…”

Section: Introductionmentioning

confidence: 99%

Pilot study of gingival connective tissue responses to 3-dimensional collagen nanofiber-coated dental implants

2015

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The aim of the present study was to evaluate the gingival connective tissue response to screw-type titanium implants coated with Type I collagen nanofibers, which were prepared using the electrospray deposition method. Implants were immediately inserted into the socket of maxillary first molars after the extraction. Undecalcified sections after 4 weeks implantation were histologically observed. Better contact of the gingival connective tissue was generally observed around the collagen nanofiber-coated implants than titanium and non-fibrous collagen-immobilized implants. Gingival connective tissue to implant contact was significantly greater with the collagen nanofiber-coated implants than with the titanium and collagen-immobilized implants at the distal side, but not at the mesial side. Polarized light microscopy revealed that some birefringent collagen fiber bundles are oriented perpendicularly to the implant surfaces in the gingival connective tissue adjacent to the collagen nanofiber-coated implants. Collagen nanofiber-coating may have a possibility for improving gingival connective tissue response to titanium implants.

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In vitro Study of Collagen Coating of Titanium Implants for Initial Cell Attachment.

Cited by 76 publications

References 20 publications

Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

Pilot study of gingival connective tissue responses to 3-dimensional collagen nanofiber-coated dental implants

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