In vitro study of the effect of zinc oxide nanoparticles on Streptococcus mutans isolated from human dental caries

Abstract

Dental caries is a public health concern worldwide for which Streptococcus mutans has been reported Known as the potential etiology of infection. In recent years, nanotechnology has applied to Creation of novel material properties. The research was studying the effects of Nanoparticles of Zinc Oxide on Streptococcus mutans isolate from dental caries. In this research, Different ZnO NPs concentrations were studied as its anti-bacterial effects on Streptococcus mutans was isolated from dental caries cases. 27 Streptococcus mutans isolates were obtained, which is equivalent to 20% of the total number of 135 isolates. This nanomaterial was chosen because it has a high affinity for human cells and does not cause harm compared to other nanoparticles. The nanoparticles are those very fine materials that can be produced so that their dimensions or the dimensions of their grains range from (1-100) nanometers, and because of the small size of the nanomaterials, which made them behave differently from large-sized materials whose size exceeds 100 nanometers, and because of the small size of the particles. Nanoparticles, which gave them electrical and magnetic properties that differ from large particles of the same compounds. The well diffusion method and the MIC experiment were conducted, and the results were good, as in the well diffusion method the efficacy increased with increasing the concentration of ZnO NPs. In the MIC experiment, it was observed that MIC in the sixth tube, meaning that the MIC of ZnO NPs towards Streptococcus mutans equals 0. 312 mg / ml.

Efficacy of zinc oxide nanoparticles and Bifidobacterium bifidum Extraction on anaerobic bacteria isolated from patients with diarrhea

“…The antibacterial activities of Zn NPs were investigated by determining the lowest inhibitory concentration against E.coli bacteria, which was corroborated by (26). Diffusion in the pits was used to monitor the antibacterial nanoparticle's quantitative assessment, and it was shown that the area of inhibition mostly relied on the concentration and agreement with results by the (14). It showed that bacterial inhibition increased as ZnO NP concentration was raised.…”

“…Then, 100 μl of each treatment were added to ZnO NPs and Bifidobacterium bifidum at a concentration of 2 mg/ml, and the plates were incubated at 37 °C for 24 hours. Next, the inhibition zone was evaluated 14 .…”

UJI AKTIVITAS ANTIBAKTERI GEL NANOPARTIKEL EKSTRAK ETANOL GAMBIR TERHADAP BAKTERI PENYEBAB KARIES GIGI (Streptococcus mutans)

“…Hasil ini sejalan dengan penelitian (Afrasiabi et al, 2020;Wassel & Khattab, 2017) yang menunjukan beberapa senyawa bahan alam seperti propolis dan siwak efektif menghambat pertumbuhan dan mengurangi biofilm yang dibentuk oleh bakteri S. Mutans. Selain dari bahan alam, logam nanopatikel seperti emas, perak dan seng juga memperlihatkan aktivitas yang sangat baik dalam menghambat pertumbuhan bakteri S. Mutans (Espinosa-Cristóbal et al, 2018;Hamad & Mahmood Atiyea, 2021;Sadony & Abozaid, 2020).…”

Metal and Metal Oxide Nanoparticles in Caries Prevention: A Review

“…Zinc oxide nanoparticles have better antimicrobial activity than other zinc nanoparticles [42,43]. In addition, zinc oxide nanoparticles inhibit growth of S. mutans [44]. The nanoparticles can be added to restorative materials to prevent caries.…”

“…• Inhibiting growth of S. mutans[44,[50][51][52], S. aureus[46], S. sobrinus[48], and oral biofilm[52];•Uncompromising mechanical properties[51,52] and bond strength[59].…”