In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces

Källtorp, Mia; Oblogina, S; Jacobsson, Stefan; Karlsson, Anna; Tengvall, Pentti; Thomsen, Peter

doi:10.1016/s0142-9612(99)00115-5

Cited by 55 publications

(42 citation statements)

References 45 publications

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“…Bioincompatibilty is often associated with increased release of proinflammatory cytokines (19). For example, in vitro studies have demonstrated that surface chemistry may influence the production and secretion of two known proinflammatory cytokines IL-1␤ and tumor necrosis factor (TNF)-␣ (18).…”

Section: Resultsmentioning

confidence: 99%

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Allen

Fox

Blute

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The assessment of biomaterial compatibility relies heavily on the analysis of macroscopic cellular responses to material interaction. However, new technologies have become available that permit a more profound understanding of the molecular basis of cellbiomaterial interaction. Here, both conventional phenotypic and contemporary transcriptomic (DNA microarray-based) analysis techniques were combined to examine the interaction of cells with a homologous series of copolymer films that subtly vary in terms of surface hydrophobicity. More specifically, we used differing combinations of N-isopropylacrylamide, which is presently used as an adaptive cell culture substrate, and the more hydrophobic, yet structurally similar, monomer N-tert-butylacrylamide. We show here that even discrete modifications with respect to the physiochemistry of soft amorphous materials can lead to significant impacts on the phenotype of interacting cells. Furthermore, we have elucidated putative links between phenotypic responses to cell-biomaterial interaction and global gene expression profile alterations. This case study indicates that high-throughput analysis of gene expression not only can greatly refine our knowledge of cell-biomaterial interaction, but also can yield novel biomarkers for potential use in biocompatibility assessment.

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Section: Resultsmentioning

confidence: 99%

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Allen

Fox

Blute

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…In those reports, -OH bearing surfaces were found to possess poor biocompatibility. 67,70,[72][73][74][75] A possible explanation for this phenomenon could be the activation of complement proteins, particularly C3b [Tang 1997]. C3b covalently links with the nucleophilic -OH group activating the alternative pathway in the complement system [McNally 1994;Hirata 2003].…”

Section: Discussionmentioning

confidence: 99%

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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Implant-associated fibrotic capsule formation presents a major challenge for the development of long term drug release microspheres and implantable sensors. Since material properties have been shown to affect in vitro cellular responses and also to influence short term in vivo tissue responses, we have thus assumed that the type and density of surface chemical groups would affect the degree of tissue responses to microsphere implants. To test this hypothesis, polypropylene particles with different surface densities of -OH and -COOH groups, along with the polypropylene control (-CH 2 groups) were utilized. The influence of functional groups and their surface densities on tissue reactions were analyzed using a mice subcutaneous implantation model. Our comparative studies included determination and correlation of the extents of fibrotic capsule formation, cell infiltration into the particles and recruitment of CD11b+ inflammatory cells for all of the substrates employed. We have observed major differences among microspheres coated with different surface functionalities. Surfaces with -OH surface groups trigger the strongest responses while -COOH rich surfaces prompt the least tissue reactions. However, variation of the surface density of either functional group has a relatively minor influence on the extent of fibrotic tissue reactions. The present results show that surface functionality can be used as a powerful tool to alter implant-associated fibrotic reactions and, potentially, to improve the efficacy and function of drug delivery microspheres, implantable sensors and tissue engineering scaffolds.

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“…[120] IL-1α, IL-1β, TNF-α, and IL-10 were quantified at biomaterial implant sites by enzyme linked immunosorbent assay (ELISA). [3,131] We have recently started using high throughput immunoassays to quantify a multitude of cytokines/chemokines at biomaterial implant sites (unpublished results).…”

Section: Crosstalk Between Macrophages/fbgcs and Inflammatorymentioning

confidence: 99%

Foreign body reaction to biomaterials

Anderson

Rodriguez

Chang

2008

Seminars in Immunology

4,260

4,024

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The foreign body reaction composed of macrophages and foreign body giant cells is the end-stage response of the inflammatory and wound healing responses following implantation of a medical device, prosthesis, or biomaterial. A brief, focused overview of events leading to the foreign body reaction is presented. The major focus of this review is on factors that modulate the interaction of macrophages and foreign body giant cells on synthetic surfaces where the chemical, physical, and morphological characteristics of the synthetic surface are considered to play a role in modulating cellular events. These events in the foreign body reaction include protein adsorption, monocyte/ macrophage adhesion, macrophage fusion to form foreign body giant cells, consequences of the foreign body response on biomaterials, and cross-talk between macrophages

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In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces

Cited by 55 publications

References 45 publications

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Foreign body reaction to biomaterials

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