Incorporation of radioactive ³⁵SO²⁻₄ into immunoreactive pituitary lutropin

Abstract

The terminal hexosamines of bovine pituitary lutropin are thought to contain a sulfate moiety. In order to test this, a biosynthetic approach was adopted. When rat and buffalo (bovine) pituitaries were incubated with radioactive 35SOi -for 2 h in vitro it was observed that radioactivity gets incorporated into trichloroacetic acid-precipitable proteins. When the radioactive proteins were treated with an anti-sheep lutropin serum, radoactivity was found in the immunoprecipitate.The incorporation into rat lutropin like material was very marginal while it was very significant in the case of buffalo lutropin.

Indigenous Production of Bovine/Bubaline Reproductive Hormones

“…The five most important pituitary protein hormones (FSH, LH, TSH, GH and PRL) which have roles to play in reproductive processes were purified from freshly frozen abattoir collected pituitaries and characterized [4][5][6][7][8]. Polyclonal and monoclonal antibodies to these hormones were raised and characterized and used to develop RIAs and ELISAs.…”

“…Some of these have been applied to field samples also [9][10]. We were rewarded with some basic discoveries during these efforts [4,[11][12][13][14][15]. Few remarks on these findings should be relevant here.…”