Influence of dietary medium- and long-chain triglycerides on fat deposition and lipogenic enzyme activities in rats.

Abstract

The present study investigates fat deposition, variances of fatty acid (FA) composition, and lipogenic enzyme activities through dietary medium- and long-chain triglyceride (MCT and LCT) supplementation in growing rats. Eighteen male Wistar rats were divided into three groups and fed isocalorically for 4 weeks with control (based on AIN 76), MCT (C8:0 26%), or LCT (corn oil 25%) diets. Compared to the control group with 0.28 +/- 0.01, feed efficiency was lower in the MCT rats and greater in the LCT rats (0.24 +/- 0.01 and 0.33 +/- 0.01, respectively). Weights of perirenal and epididymal adipose tissue pads of the MCT rats were similar to those of the control group, but were significantly lower than those of the LCT group. Whole-body carcass components data of MCT rats showed the decrease in moisture and protein contents compared to those of control and LCT rats. Fat content of LCT rats was 25-30% higher than those of the MCT and control group. Glucose-6-phosphate dehydrogenase, citrate cleavage enzyme, and malic enzyme activities of liver and epididymal adipose tissue were markedly low in LCT rats. In the MCT group, however, lipogenic enzyme activities were not suppressed, and malic enzyme activity was drastically increased. FA composition of whole-body triglycerides and epididymal adipose tissue in MCT rats showed that C16:0 and C16:1 levels were higher than those of the LCT rats. In contrast, FA composition of the LCT group presented high C18:2 content.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of GPR84 deletion on obesity and diabetes development in mice fed long chain or medium chain fatty acid rich diets

“…This corroborates a previous finding in rats, that described that WAT accumulates more in MCFA than control diet fed animals, despite no difference in body mass [5]. However a contradictory finding in a rat study demonstrated that epididymal mass was similar in animals fed control diet or medium chain triglyceride-rich diets, compared to enlarged epididymal fat mass in animals fed long chain triglycerides [31]. We found no observable GPR84 genotype-specific effect on body fat accumulation, suggesting that differences in epididymal fat accumulation are not dependent on GPR84.…”

Effect of Medium-Chain Fatty Acids-Containing Dietary Oil on Hepatic Fatty Acid Oxidation Enzyme Activity in Rats.

“…Conversely, Takase and Hosoya reported that three days of a diet containing MCT (probably with the same amount of MCFA as in our study) caused an enhancement of fatty acid synthase and malic enzyme, which was related with fatty acid lipogenesis similar to fatty acid synthase in Wistar rat livers (20). Similar results have been reported by other investigators (21)(22)(23)(24). These results indicated that (excess) MCFA could enhance lipogenic activity under certain condition, and our results suggested that the coexistence of LCFA decreased the lipogenic activity of MCFA.…”

Effect of Randomly Interesterified Triacylglycerols Containing Medium- and Long-Chain Fatty Acids on Energy Expenditure and Hepatic Fatty Acid Metabolism in Rats

“…Other reports have indicated the ability of MCT to enhance de novo lipogenesis in the liver. [35][36][37][38] It is thought that the magnitude of the enhancement in lipogenesis by MCFA might be one reason for the inconsistency in demonstrating a body fat-lowering effect of MCT. Its effect appears to depend on experimental conditions and the characteristics of subjects.…”