Influence of high-fat diet on bone tissue: An experimental study in growing rats

Yanagihara, Gabriela Rezende; Shimano, Roberta Carminati; Tida, Jacqueline Atsuko; Yamanaka, Jéssica Suzuki; Fukada, Sandra Yasuyo; Issa, João Paulo Mardegan; Shimano, Antonio Carlos; Tavares, João Manuel R. S.

doi:10.1007/s12603-017-0871-x

Cited by 13 publications

(9 citation statements)

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“…1a and 1b, respectively, in supplementary information). These data corroborate with the literature carried out with humans 29 and rats 30 since obese people have greater muscle and bone overload. It is worth mentioning that, at the end of the experiment, these animals were middle-aged, and possibly with no signs of sarcopenic obesity appearing, which can affect both the loss of muscle and bone mass 31 .…”

Section: Discussionsupporting

confidence: 92%

Prospective study on intracranial pressure waveform morphology and its relationship with the development of obesity in rats

Marine¹,

Alves²,

Sene-Fiorese³

et al. 2021

Preprint

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Obesity accumulates fat in the body may cause hypertension and change intracranial pressure waveform morphology (ICP). However, not clear how and when it occurs. Thus, our objective was to evaluate the ICP waveform morphology and P2/P1 ratio during the development of obesity. Sixteen 45-day-old male Wistar rats, after 60 days of adaptation were divided into non-obese (NOB, n = 7) and obese (OB, n = 9). The OB group received a high-fat diet and the NOB group received a standard diet for 24 weeks. ICP, body composition, blood pressure (BP), and heart rate(HR) were assessed every 4 weeks. Two-Way ANOVA was used for the area under the curve with Bonferroni's test, or Student's t-test for BP, and HR. Body mass(BM) and body fat(BF) and fat-free mass(FFM) were higher in OB rats. There was no difference in BP, but there was a difference in HR, greater in OB. The ICP decreased by NOB. These results suggest that the increase in BM and BF caused obesity, and BM can explain the increase in FFM. Although we did not find pathological states of ICP, NOB demonstrated better brain compliance, and the upward trend in the P2 waveform in OB may indicate impairment to the ICP of OB rats.

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Section: Discussionsupporting

confidence: 92%

Prospective study on intracranial pressure waveform morphology and its relationship with the development of obesity in rats

Marine¹,

Alves²,

Sene-Fiorese³

et al. 2021

Preprint

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“…The left femora were submitted to densitometric analysis using “dual-energy” x-ray densitometer equipment, Alpha, Lunar DPX ® (Lunar, Madison, WI, United States) installed at the School of Medicine of Ribeirao Preto. Adapted for measurement small animals with the PIXImus software (Lunar, Madison, WI, United States) 49,50 . The image acquisition is proceeded with the femurs (distal portion of the femur) position with ROI of 9 mm² (Fig.…”

Section: Methodsmentioning

confidence: 99%

Doxycycline reduces osteopenia in female rats

Figueiredo

Shimano

Ervolino

et al. 2019

Sci Rep

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Doxycycline, a member of the tetracycline family, is a drug used as an antibiotic (dosage of 100 mg/day) and as an anti-inflammatory drug on the dosage of 20 mg twice a day, this use has Matrix Metalloproteinases (MMP) inhibitor action. Doxycycline is a calcium chelator and therefore interferes in bone remodeling. The main objective of this study was to evaluate the action of the drug doxycycline in the control of osteopenia. Sixty three Wistars rats were divided into 9 groups with n = 7 each, as follow: the control group with doxycycline 10 mg/kg/day (C10), control with doxycycline 30 mg/kg/day (C30) and control (C), ovariectomized group with doxycycline 10 mg/kg/day (OVX10), ovariectomized with doxycycline 30 mg/kg/day (OVX30), and ovariectomized with water (OVX), sedentary group with 10 mg/kg/day (Se10), sedentary with doxycycline 30 mg/kg/day (Se30), and sedentary group with water (Se). Left femoral bone was used for bone densitometry, right femoral bone for histological analysis. The right tibia was intended for chemical quantifications, the total serum was used for cholesterol and calcium quantification. The length of the left femoral bone was measured after the densitometry analysis. Statistical analysis was performed using multivariate general linear model (ANOVA two factors with Bonferroni adjustment) and the TRAP analysis was subjected to normality test and then were subjected to nonparametric test, both with p < 0.05 significance. Statistically significant differences were found, with better results for the groups exposed to the medication (10 and 30 mg/kg/day): Se vs. Se10 and Se vs. Se30 for BMC, quantification of magnesium, amount of cancellous bone in the distal portion; OVX vs. OVX10 for BMC, BMD and calcium in serum; OVX vs. OVX10 and OVX30 for quantification in proximal and distal portion of cancellous bone; Se vs. Se30 and OVX vs. OVX30 for immunostaining for TRAP, all results with minimum of p ≤ 0.05. Doxycycline had a deleterious effect on control groups and positive action for bone organization on female rats affected by bilateral ovariectomy-induced osteopenia and sedentary lifestyle.

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“…Studies carried out on animals (30)(31)(32)(33) and humans (34) indicated that the amount and source of dietary fat may affect bone metabolism by changing Ca absorption, osteoblast formation, lipid oxidation and synthesis of pro-and anti-inflammatory eicosanoids. However, the aim of this study was to compare the influence of an unbalanced ratio of lysine:ME and elevated consumption of SFA (similar to the Western-type diet in human nutrition) on bone properties.…”

Section: Composition Of Pig Dietsmentioning

confidence: 99%

Inulin supplementation reduces the negative effect of a high-fat diet rich in SFA on bone health of growing pigs

2018

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Consumption of a high-fat diet, rich in SFA, causes deterioration of bone properties. Some studies suggest that feeding inulin to animals may increase mineral absorption and positively affect bone quality; however, these studies have been carried out only on rodents fed a standard diet. The primary objective of this study was to determine the effect of inulin on bone health of pigs (using it as an animal model for humans) fed a high-fat diet rich in SFA, having an unbalanced ratio of lysine:metabolisable energy. It was hypothesised that inulin reduces the negative effects of such a diet on bone health. At 50 d of age, twenty-one pigs were randomly allotted to three groups: the control (C) group fed a standard diet, and two experimental (T and TI) groups fed a high-fat diet rich in SFA. Moreover, TI pigs consumed an extra inulin supply (7 % of daily feed intake). After 10 weeks, whole-body bone mineral content (P=0·0054) and bone mineral density (P=0·0322) were higher in pigs of groups TI and C compared with those of group T. Femur bone mineral density was highest in pigs in group C, lower in group TI and lowest in group T (P=0·001). Femurs of pigs in groups TI and C had similar, but higher, maximum strength compared with femurs of pigs in group T (P=0·0082). In conclusion, consumption of a high-fat diet rich in SFA adversely affected bone health, but inulin supplementation in such a diet diminishes this negative effect.

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Influence of high-fat diet on bone tissue: An experimental study in growing rats

Abstract: Liver and gene parameters are changed in response to the HFD. However, although there was an increase in BMD, the microstructure and function of the bone did not change after a 5-week HFD.

Cited by 13 publications

References 50 publications

Prospective study on intracranial pressure waveform morphology and its relationship with the development of obesity in rats

Prospective study on intracranial pressure waveform morphology and its relationship with the development of obesity in rats

Doxycycline reduces osteopenia in female rats

Inulin supplementation reduces the negative effect of a high-fat diet rich in SFA on bone health of growing pigs

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