Influence of nitrogen availability on the biochemical composition of three unicellular marine algae of commercial importance

Utting, Susan D.

doi:10.1016/0144-8609(85)90012-3

Cited by 71 publications

(44 citation statements)

References 13 publications

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“…Often this excess accumulates as fatty acids (Roessler, 1990), as is documented for Isochrysis (Utting, 1985;Ben-Amotz et al, 1985). The results from this study, consistent with our previous observations, show that during N-deprivation the cellular content of C does not increase, only the N content decreases.…”

Section: Discussionsupporting

confidence: 93%

Changes in fatty acids, amino acids and carbon/nitrogen biomass during nitrogen starvation of ammonium- and nitrate-grownIsochrysis galbana

et al. 1992

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Growth of cells of Isochrysis galbana with either nitrate or ammonium as the N-source, and the effects of subsequent N-starvation of these cells, were compared. During exponential N-sufficient growth nitrate-grown cells had double the fatty acid content of the ammonium-grown cells but lower concentrations of a few amino acids. Following resuspension in N-free medium the fatty acid content of the ammonium-grown cells increased to that of the nitrate-grown cells, but there was no further increase in fatty acid content on a C-biomass or cellular basis during the following 4 days for either culture. Fatty acid synthesis was continuous during N-starvation, while it occurred during the light-phase only in exponential growth. The proportion of 18: ln9 fatty acid increased from 10 to 25% total fatty acids during N-starvation. Intracellular free amino acid content decreased in a similar manner in both cultures on N-starvation, the ratio of intracellular free amino-N/cell-C falling more rapidly than overall cellular N/C. It was concluded that optimal amino acid and fatty acid content would be attained by growth in the presence of excess nitrate. Measurements of chlorophyll and carotenoid content and of in vivo fluorescence indicated that these parameters had potential for monitoring the C and N biomass in cultures grown under relatively constant (not necessarily continuous) illumination.

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Section: Discussionsupporting

confidence: 93%

Changes in fatty acids, amino acids and carbon/nitrogen biomass during nitrogen starvation of ammonium- and nitrate-grownIsochrysis galbana

et al. 1992

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“…It is known that the lipid content of L galbana increases when the culture ages (Utting 1985), that is to say with the growth phase. Also the lipid content of microalgae is inversely proportional to growth rate (Piorreck et al 1984), which is in agreement with the earlier statement.…”

Section: Biochemical Compositionmentioning

confidence: 99%

n-3 PUFA productivity in chemostat cultures of microalgae

Grima

Pérez²,

Camacho

et al. 1993

Appl Microbiol Biotechnol

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Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid productivities from chemostat cultures of an isolate of Isochrysis galbana have been studied. The productivities reached in the interval of dilution rates between 0.0295h-1 and 0.0355h-1 were 1 . 5 m g . l -l . h -~ for lipids, 3 0 0 g g . l -~. h -a for EPA and 130 gg.l -a . h -1 for DHA. Furthermore, light attenuation by mutual shading, and agitation speed influences on growth and fatty acid composition were analysed. A model relating steady-state dilution rates to internal average light intensity has been proposed, the parameter values of which obtained by non-linear regression were: maximum specific growth rate (~ma×)=0.0426h-~; the affinity of cells to light (Ik) = 10.92 W" m -z; the exponent (n) = 5.13; regression coefficient (r 2) = 0.9999.

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“…Carbohydrates were measured by the phenol-sulfuric acid method (Kochert, 1978). Dry weight was determined using a Millipore © filtration system, with 0.45 µm fiberglass filter, by the method of Utting (1985).…”

Section: Methodsmentioning

confidence: 99%

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

et al. 2016

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Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO 3 . Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaena sp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL -1 at 8.5 mM NaNO 3 . For chlorophyll, phycocyanin and phycoerythrin, maximum values were achieved at 17 mM NaNO 3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL -1 , respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL -1 , and 890 mg mL -1 of carbohydrates in the absence of nitrogen. This comparative study shows that the most efficient strain for the production of protein, carbohydrates and lipids in diazotrophic conditions corresponded to Nostoc LAUN0015. However, Anabaena sp.1 and Anabaena sp.2 required high nitrogen concentrations to achieve higher values of metabolites, comparing with Nostoc strains. Nitrogen dependence for the production of pigments and high protein production in strains of Anabaena and in diazotrophic conditions for Nostoc was demonstrated. Nostoc can be cultured under nitrogen deficiency and Anabaena in sufficiency, for biomass production enriched with proteins and carbohydrates.

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Influence of nitrogen availability on the biochemical composition of three unicellular marine algae of commercial importance

Cited by 71 publications

References 13 publications

Changes in fatty acids, amino acids and carbon/nitrogen biomass during nitrogen starvation of ammonium- and nitrate-grownIsochrysis galbana

Changes in fatty acids, amino acids and carbon/nitrogen biomass during nitrogen starvation of ammonium- and nitrate-grownIsochrysis galbana

n-3 PUFA productivity in chemostat cultures of microalgae

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

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