Influence of pressure and temperature on the physico-chemical properties of mobile phase mixtures commonly used in high-performance liquid chromatography

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

“…The temperature was measured at the flow rates 0.25, 0.50, 1.00 and 1.20 mL/min for UHPLC and at 0.40 and 1.00 mL/min for HPLC. The mobile phase was 25% acetonitrile as this composition corresponds to the largest viscosity of the mobile phase [27].…”

Theoretical evaluation of the advantages and limitations of constant pressure versus constant flow rate gradient elution separation in supercritical fluid chromatography

“…While in HPLC the density is nearly constant (except for ultra-high performance liquid chromatography conditions [6]), the mobile phase undergoes a significant decompression along the column axis in SFC [4,7]. This has a significant influence on retention.…”

A study of mass transfer kinetics of alanyl-alanine on a chiral crown ether stationary phase

“…Both Á(P) and D m (P) functions are close to linear within the mentioned pressure interval. Empirical data on an Á(P) dependence needed for these calculations were found in the literature [31].…”

“…All the retention data below were corrected correspondingly. In these calculations, we used a (P) function published in [31]. The magnitude of the correction factor did not exceed 1% at the highest flow rate studied.…”