Influence of reactor systems on the morphology of Aspergillus awamori. Application of neural network and cluster analysis for characterization of fungal morphology

Gerlach, Stephanie; Siedenberg, D.; Gerlach, Deidra L.; Schügerl, K.; Giuseppin, Marco L. F.; Hunik, J.H.

doi:10.1016/s0032-9592(98)00016-8

Cited by 32 publications

(26 citation statements)

References 32 publications

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“…Many reports claim that the differing morphology of fungi mycelia under different pH values was the critical factor for mycelial accumulation and metabolite formation [26,27]. The initial pH of culture medium may affect cell membrane function, cell morphology and structure, and product formation [26,28]. Thus, the initial pH at 5 was determined in terms of the beneficial effect on EPS production in further studies.…”

Section: Resultsmentioning

confidence: 99%

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Chen

et al. 2010

Bioprocess Biosyst Eng

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This work aimed to develop the submerged cultivation conditions for improved exopolysaccharides (EPS) production by Armillaria luteo-virens Sacc. The effects of culture temperature, aeration rate, inoculum level, initial pH, and additives on EPS formation and mycelial growth are investigated. The aeration rate, initial pH, and inoculum level significantly affected EPS production under the submerged cultivation. The developed conditions were as follows: cultivation temperature 23 °C, initial pH 5.0, aeration rate 0.5 vvm, 0.5% Tween 80, inoculum level 5% (v/v), and shaking speed 120 r/min. Under the developed conditions, the highest EPS production was 13.01 g/L at 5 days culture time. EPS production was examined in a 5 L bioreactor, and an unstructured kinetic model for EPS formation was well developed. The verified investigations in the large-scale cultivation system showed that the developed models are able to predict the submerged cultivation process of EPS formation. Current results revealed that the submerged cultivation conditions can be utilized to control EPS production, and the unstructured models developed are suitable for explaining EPS production by A. luteo-virens Sacc QH in a large-scale cultivation bioreactor.

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Section: Resultsmentioning

confidence: 99%

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Chen

et al. 2010

Bioprocess Biosyst Eng

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“…pH could affect mycelial cell membrane function, cell morphology and structure, the uptake of various nutriments, and product biosynthesis (Gerlach et al, 1998). The optimum values of pH for various edible mushrooms were different.…”

Section: Discussionmentioning

confidence: 99%

Effects of culture conditions on mycelium biomass and intracellular cordycepin production ofCordyceps militaris in natural medium

Xie

Liu

et al. 2009

Ann. Microbiol.

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-Effects of culture conditions on the growth of mycelium and the production of intracellular cordycepin of Cordyceps militaris were investigated in this study. The natural medium which is a mixture of 53% brown rice paste, 6% beerwort and 42% soybean meal juice was used for the fermentation in shaking flasks. Fermentation temperature, pH and medium capacity, which had been proved to be significant factors, were optimized by Box-Behnken design. Results showed that dry mycelia weight (DMW) and cordycepin yield (CY) was varied with the transformation of culture conditions. The highest DMW (19.1 g/L) and CY (1.8 mg/g) would be obtained at the condition of fermentation temperature 28 °C, pH 6.2 and medium capacity 57 mL.

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“…The morphology of Aspergillus, filamentous growth forms and/or the shape and size of pelleted growth forms as well as the branching frequency, is influenced by many factors including specific strain properties [22] but also environmental factors such as spore numbers in the inoculum or type of inoculum [23][24][25], medium composition [23,[26][27][28], dissolved oxygen concentration [29][30][31], pH [23,32], temperature [23,27], reactor type and scale [27,33], and power input [23,25,26,28,29,[33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger

El‐Enshasy

Kleine

Rinas

2006

Process Biochemistry

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Recombinant Aspergillus niger genetically engineered to produce glucose oxidase using the constitutive gpdA promoter and the glucoamylase signal sequence for secretion was grown in batch cultures at agitation speeds of 200 -800 rpm covering the industrial relevant power input range of 0.1 -5 W kg -1 . The growth morphology ranged from large pellets with an average diameter of 1500 µm at low power input up to micropellets embedded in a filamentous network at high power input. A correlation of agitation intensity with growth morphology and glucose oxidase production revealed an increase of the protein production capability with the change of the growth morphology from pelleted to filamentous growth forms. However, the exposure to higher shear stress with increasing power input also resulted in lower biomass yields as well as increased transient formation of polyol (xylitol) and higher final concentrations of oxalic acid. The highest specific production rates were found in young filamentous growth forms at high power input. Although intermediate agitation intensity leading to small pellets became more favorable during prolonged cultivation. An acridine orange staining procedure discriminating between RNA rich (red) and RNA poor regions (green) of the fungal biomass proved that active protein production is restricted to filamentous growth forms and the outer layer of fungal pellets. A correlation between the RNA rich fraction of the biomass determined by image analysis and the productivity is shown.

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Influence of reactor systems on the morphology of Aspergillus awamori. Application of neural network and cluster analysis for characterization of fungal morphology

Cited by 32 publications

References 32 publications

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Effects of culture conditions on mycelium biomass and intracellular cordycepin production ofCordyceps militaris in natural medium

Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger

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