Influence of tryptophan and indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokiniana–Azospirillum brasilense system under heterotrophic conditions

Palacios, Oskar A.; Choix, Francisco J.; Bashan, Yoav; de‐Bashan, Luz E.

doi:10.1016/j.resmic.2016.02.005

Cited by 32 publications

(13 citation statements)

References 72 publications

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“…Some MGPB can increase the content of lipids [24, 45–47], fatty acids [47], starch [48], or hydrocarbons [32] in microalgal cells. A. brasilense Cd can significantly increase the accumulation of fatty acids and lipids in C. vulgaris [45, 47].…”

Section: Discussionmentioning

confidence: 99%

“…Co-immobilization of a microalga-MGPB symbiosis has been proposed as a promising and effective strategy for enhancing microalgal abilities and for use in wastewater treatment and biomass production [49]. C. vulgaris has been co-immobilized with A. brasilense Cd in alginate beads, and the symbiosis has resulted in (1) enhanced nutrient removal in synthetic wastewater compared to immobilized C. vulgaris alone, and (2) enhanced biomass, lipid, and starch productivities in synthetic media [45, 48, 50]. In this study, we proposed a two-step culture system as another application of MGPB to enhance biomass production of E. gracilis .…”

Section: Discussionmentioning

confidence: 99%

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Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Toyama

Hanaoka

Yamada³

et al. 2019

Biotechnol Biofuels

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BackgroundEuglena gracilis, a unicellular flagellated microalga, is regarded as one of the most promising species as microalgal feedstock for biofuels. Its lipids (mainly wax esters) are suitable for biodiesel and jet fuel. Culture of E. gracilis using wastewater effluent will improve the economics of E. gracilis biofuel production. Enhancement of the productivity of E. gracilis biomass is critical to creating a highly efficient biofuels production system. Certain bacteria have been found to promote microalgal growth by creating a favorable microenvironment. These bacteria have been characterized as microalgae growth-promoting bacteria (MGPB). Co-culture of microalgae with MGPB might offer an effective strategy to enhance microalgal biomass production in wastewater effluent culture systems. However, no MGPB has been identified to enhance the growth of E. gracilis. The objectives of this study were, therefore, to isolate and characterize the MGPB effective for E. gracilis and to demonstrate that the isolated MGPB indeed enhances the production of biomass and lipids by E. gracilis in wastewater effluent culture system.ResultsA bacterium, Emticicia sp. EG3, which is capable of promoting the growth of microalga E. gracilis, was isolated from an E. gracilis-municipal wastewater effluent culture. Biomass production rate of E. gracilis was enhanced 3.5-fold and 3.1-fold by EG3 in the co-culture system using a medium of heat-sterilized and non-sterilized wastewater effluent, respectively, compared to growth in the same effluent culture but without EG3. Two-step culture system was examined as follows: E. gracilis was cultured with or without EG3 in wastewater effluent in the first step and was further grown in wastewater effluent in the second step. Production yields of biomass and lipids by E. gracilis were enhanced 3.2-fold and 2.9-fold, respectively, in the second step of the system in which E. gracilis was co-cultured with EG3 in the first step.ConclusionEmticicia sp. EG3 is the first MGPB for E. gracilis. Growth-promoting bacteria such as EG3 will be promising agents for enhancing E. gracilis biomass/biofuel productivities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Toyama

Hanaoka

Yamada³

et al. 2019

Biotechnol Biofuels

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“…Microalgae-bacteria interactions have been artificially induced with soil-borne bacteria strains that possess plant-growthpromoting (PGP) activity in terrestrial environments. For example, the interaction of the soil-borne bacteria Azospirillum brasilense with the microalgae Chlorella sorokiniana was found to increase photosynthetic activity, population density, and accumulation of cellular compounds in the microalgae (Palacios et al, 2016;Amavizca et al, 2017). This biostimulant activity of A. brasilense was further shown to be due to a constant provision of the phytohormone indoleacetic acid (IAA) by the bacteria (De-Bashan et al, 2008;Choix et al, 2014;Palacios et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants

et al. 2020

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“…Auxin (IAA), abscisic acid (ABA), brassinosteroid (BR), and gibberellin (GA) play important roles in starch biosynthesis and grain development. Palacios et al [14] found that the AGP activity and starch content in Chlorella sorokiniana strains with normal levels of IAA accumulation were higher than in those with abnormal levels of IAA accumulation. In Arabidopsis , the sucrose induced expression of AGPL3 and SBE2.2 could be enhanced by ABA [15].…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptome, small RNA, and degradome analysis reveals the complex network regulating starch biosynthesis in maize

Zhang¹,

Xie²,

Han³

et al. 2019

BMC Genomics

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Background Starch biosynthesis in endosperm is a key process influencing grain yield and quality in maize. Although a number of starch biosynthetic genes have been well characterized, the mechanisms by which the expression of these genes is regulated, especially in regard to microRNAs (miRNAs), remain largely unclear. Results Sequence data for small RNAs, degradome, and transcriptome of maize endosperm at 15 and 25 d after pollination (DAP) from inbred lines Mo17 and Ji419, which exhibit distinct starch content and starch granule structure, revealed the mediation of starch biosynthetic pathways by miRNAs. Transcriptome analysis of these two lines indicated that 33 of 40 starch biosynthetic genes were differentially expressed, of which 12 were up-regulated in Ji419 at 15 DAP, one was up-regulated in Ji419 at 25 DAP, 14 were up-regulated in Ji419 at both 15 and 25 DAP, one was down-regulated in Ji419 at 15 DAP, two were down-regulated in Ji419 at 25 DAP, and three were up-regulated in Ji419 at 15 DAP and down-regulated in Ji419 at 25 DAP, compared with Mo17. Through combined analyses of small RNA and degradome sequences, 22 differentially expressed miRNAs were identified, including 14 known and eight previously unknown miRNAs that could target 35 genes. Furthermore, a complex co-expression regulatory network was constructed, in which 19 miRNAs could modulate starch biosynthesis in endosperm by tuning the expression of 19 target genes. Moreover, the potential operation of four miRNA-mediated pathways involving transcription factors, miR169a- NF-YA1 - GBSSI / SSIIIa and miR169o- GATA9 - SSIIIa / SBEIIb , was validated via analyses of expression pattern, transient transformation assays, and transactivation assays. Conclusion Our results suggest that miRNAs play a critical role in starch biosynthesis in endosperm, and that miRNA-mediated networks could modulate starch biosynthesis in this tissue. These results have provided important insights into the molecular mechanism of starch biosynthesis in developing maize endosperm. Electronic supplementary material The online version of this article (10.1186/s12864-019-5945-1) contains supplementary material, which is available to authorized users.

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Influence of tryptophan and indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokiniana–Azospirillum brasilense system under heterotrophic conditions

Cited by 32 publications

References 72 publications

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants

Integrated transcriptome, small RNA, and degradome analysis reveals the complex network regulating starch biosynthesis in maize

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