Influence of various factors on the chromatographic behavior of cytostatic antibiotics of rubomicin derivatives in micellar liquid chromatography

Abstract

KeyWordsColumn liquid chromatography Micella r chromatog ra phy SodTum dodecyl sulfate Cytostatic antibiotics of rubomicin derivatives Summary Micellar ITquTd chromatography has been adopted for separation of cytostatic antibiotics of rubomidn derivatives. The influence of the kind of organic modifier (normal and iso-alcehols) and its cencentratTon, sodTum dodecyl sulfate micelle concentratTon and column temperature on the chromatographic characteristics of rubomicn derivatives have been investigated. ]_ pentanol and iso propanol were shown to be the most suitable for providing optimum selectiv ity, good taTITng factor and column efriciencywth mTnimum reagent consumption.reversed-phase chromatography. MLC provides additional possibilities to control the selectivity and retention by variation of micelle concentration, as well as organic modifier and its concentration in the mobile phase.The objective in this work is to optimize chromatographic conditions for separation and determination of rubomicin derivatives using the micellar mobile phase with sodium dodecyl sulfate (SDS). For this purpose, the influence of the surfactant concentration, pH, ionic strength, column temperature, organic modifier and its concentration on chromatographic characteristics of rubomicin derivatives were investigated.

Determination of selenium(IV) in pharmaceuticals and premixes by micellar liquid chromatography

“…It has been reported [34] that changing the type of organic modifier, for example use of normal or iso alcohols, can affect the chromatographic behavior of solutes when micellar mobile phases are used. We investigated the influence of iso-butanols as organic modifiers on 4,5-naphthylpiazselenol chromatographic behavior and its asymmetry factor and on the column efficiency ( Table 1).…”

Chromatographic behavior of epirubicin and its analogues on high-purity silica in hydrophilic interaction chromatography

“…Compared to RPLC, the normal phase retention behavior of HILIC offers the potential for dramatic changes in selectivity with quite simple mobile phase. The compositions of the eluents used in most RPLC methods are comparatively complex [15][16][17][18][19][20], and phosphate buffers as well as anionic surfactants are favored in HPLC-UV but not in MS detection. It can be expected that HILIC on silica with high-organic low-ionic-strength volatile buffer will be a good choice for developing LC-MS assay for quantifications of epirubicin and its metabolites in biological matrices.…”

“…1. Several RPLC methods have been developed for separation of epirubicin and related compounds [15][16][17][18][19][20]. In our previous work, milligram amounts of epirubicin were isolated from raw product by RPLC carried out in the overloaded elution mode [21] and in the displacement mode [22] on an analytical-scale Kromasil KR100-10C 18 column.…”