Heteroscedasticity of retention factor and adequate modeling in micellar liquid chromatography

Boichenko, Alexander P.; Iwashchenko, Anna L.; Loginova, Lidia P.; Kulikov, A. Yu.

doi:10.1016/j.aca.2006.06.016

Cited by 15 publications

(4 citation statements)

References 61 publications

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“…The weights were calculated with the following function w logk ¼ k 2 /0.434 for taking into account the perturbations after function linearization. 19 The correlation coefficient between experimental and predicted logk was higher than 0.97 for all investigated compounds. Fig.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 77%

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Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

2011

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Micellar liquid chromatography (MLC) has been developed for the analysis of opium alkaloids. The composition of micellar mobile phase for the separation of morphine, codeine, papaverine and noscapine have been optimized by a chemometric tool taking into account the efficiency and asymmetry of chromatographic peaks, resolution between each pair of peaks and total time of separation. The optimized mobile phase composition (0.10 M sodium dodecylsulfate (SDS), 5% (v/v) 1butanol; pH 2.5) allows to fully separate alkaloids within 15 min. The proposed MLC method was validated, compared with the RP-HPLC method and successfully applied for the analysis of the pharmaceutical preparation ''Omnopon, solution for injection''.

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Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 77%

“…The retention of each compound was simulated by using a bilogarithmic mechanistic retention model (eqn (1)) that follows from the physical-chemical retention model based on the mass-action concept of micelle formation. 18,19 logk…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

2011

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“…It was noted previously that the surfactant adsorbed layer on the LC packing affected drastically chromatographic retention, owing to the poor mass transfer within the stationary phase and rate constant for solute exit from the micellar phases 31–33. In addition to the impact of ACN on the micelles, we continued to explore the use of different SDS concentrations for improved efficiency in the mobile phases.…”

Section: Resultsmentioning

confidence: 99%

Micellar and aqueous‐organic liquid chromatography using sub‐2 μm packings for fast separation of natural phenolic compounds

Cao

Cheng

2010

J of Separation Science

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The objective of the present work was to investigate the chromatographic behavior of natural phenolic compounds in micellar and aqueous-organic LC using a short column packed with 1.8 microm particles. Firstly, the effect of ACN and SDS on elution strength and selectivity was examined by isocratic submicellar (0-30% ACN/5% 1-butanol/1-6 mM SDS) and micellar (0-30% ACN/5% 1-butanol/40-60 mM SDS) systems. The varied concentrations of two modifiers in the mobile phases revealed different eluting power. Then, the application of organic modifier gradient was discussed in both submicellar and micellar LC using mobile phases of 4 mM SDS/5% 1-butanol or 50 mM SDS/5% 1-butanol containing ACN gradient from 0 to 30%, respectively. For micellar system, the separation was found to be better in gradient than isocratic elution. Additionally, the sensitivity of aqueous-organic LC was examined. The mobile phase was a mixture of ACN and water employing gradient elution at a flow rate of 0.5 mL/min, with analysis time below 9 min. It was found that separation efficiency was significantly better compared with micellar LC. Besides, the aqueous-organic LC has been applied to separation of various phenolic compounds in Yangwei granule or Radix Astragali samples.

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“…Several empirical and mechanical retention models have been proposed to take into account the presence of modifiers in micellar mobile phases [22][23][24][25]. The models allowed to develop optimization procedures which can be used to find quickly the best surfactant concentration and modifier proportion in a micellar mobile phase that will give the optimal resolution in a particular situation [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Aliphatic carboxylic acids and alcohols as efficiency and elution strength enhancers in micellar liquid chromatography

Boichenko

Berthod

2010

Journal of Chromatography A

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Heteroscedasticity of retention factor and adequate modeling in micellar liquid chromatography

Cited by 15 publications

References 61 publications

Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Micellar and aqueous‐organic liquid chromatography using sub‐2 μm packings for fast separation of natural phenolic compounds

Aliphatic carboxylic acids and alcohols as efficiency and elution strength enhancers in micellar liquid chromatography

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