Inheritance of adult-plant resistance to Phytophthora capsici in pepper

Reifschneider, F. J. B.; Boiteux, L. S.; Vecchia, P. T. Della; Poulos, J. M.; Kuroda, Nagamichi

doi:10.1007/bf00036086

Cited by 60 publications

(36 citation statements)

References 12 publications

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“…Several studies have been conducted to screen resistant lines from Capsicum genetic resources, and some lines resistant to P. capsici have been found (Barksdale et al 1984, Ortega et al 1992, Reifschneider et al 1992. From these resistant resources, a local cultivar from Morelos in Mexico, 'Criollo de Morelos 334' ('CM334'), was reported to be highly resistant to P. capsici (Ortega et al 1991, Walker and Bosland 1999, Oelke et al 2003.…”

Section: Introductionmentioning

confidence: 99%

QTL Analysis for Resistance to Phytophthora capsici in Pepper Using a High Density SSR-based Map

Minamiyama¹,

Tsuro

Kubo

et al. 2007

Breed. Sci.

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A segregating doubled haploid (DH) population (n = 96) was developed by anther culture of an F 1 plant crossed between susceptible ('Manganji') and resistant ('Criollo de Morelos 334') lines of pepper (Capsicum annuum L.) to conduct a genetic analysis of resistance to Phytophthora rot caused by Phytophthora capsici. In order to perform a quantitative trait locus (QTL) analysis, we constructed a high density simple sequence repeat (SSR)-based map with a total length of 878 cM. Sixteen linkage groups (LGs) and 118 SSR markers were located using the 626 SSR markers that we previously developed. Resistance was evaluated in two root inoculation tests. Interval mapping for the resistance to P. capsici detected a common major QTL in the duplicate tests and a minor QTL specific to the first test. The major QTL was located on LG15 and flanked with an SSR marker, CAMS420. In addition, seven SSR markers were located within 21 cM intervals from the peak of this QTL. In contrast, the QTL on LG3 was detected with small effects in the first test, the nearest marker was a dominant amplified fragment length polymorphism (AFLP) marker, and the QTL was surrounded by eight SSR markers within a distance of 10 cM. Since some of the linkage markers for agriculturally valuable traits cannot detect polymorphism within breeding populations in C. annuum, the present linkage markers may widen the choice in marker-assisted selection in breeding programs for Phytophthora rot resistant pepper cultivars.

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Section: Introductionmentioning

confidence: 99%

QTL Analysis for Resistance to Phytophthora capsici in Pepper Using a High Density SSR-based Map

Minamiyama¹,

Tsuro

Kubo

et al. 2007

Breed. Sci.

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“…Some pepper accessions have been reported to be resistant to P. capsici (Smith et al 1967, Palloix et al 1990, Bartual et al 1991, Reifschneider et al 1992. In particular, two wild accessions of C. annuum, 'AC2258' derived from PI 201234 from Central America and 'Criollo de Morelos 334' ('CM334') from Mexico display a high level of resistance to P. capsici.…”

Section: Introductionmentioning

confidence: 99%

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

et al. 2006

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A doubled-haploid (DH) population (n = 176) obtained by anther culture of an F 1 hybrid between a line susceptible to Phytophthora capsici 'K9-11' (Capsicum annuum L.) and a line resistant to P. capsici 'AC2258' (C. annuum L.) was inoculated with P. capsici. QTL analysis of the resistance was performed using a linkage map consisting of 16 linkage groups (LGs), covering a total distance of 1100.5 cM. Three QTLs were detected on LG1, LG6 and LG7. The QTL with the highest LOD score, detected on LG7, explained 82.7% of the phenotypic variance with a LOD score of 67.02. This QTL was designated as Phyt-1. The nearest marker was an AFLP marker, M10E3-6. The second QTL, designated as Phyt-2, was found on LG1. It explained 6.4% of the phenotypic variance with a LOD score of 2.54. The nearest RAPD marker was RP13-1. The other QTL, designated as Phyt-3, which was found on LG6, explained 5.6% of the phenotypic variance with a LOD score of 2.20. The nearest AFLP marker was M9E3-11. It was confirmed that the lines with a high resistance could be efficiently selected by using two markers, M10E3-6 and RP13-1, simultaneously. The presence of both Phyt-1 and Phyt-2 under homozygous conditions may enable to breed resistant cultivars of sweet pepper. The molecular markers identified in the present study could be useful for marker-assisted selection (MAS) in order to breed sweet pepper cultivars with a high resistance to P. capsici using 'AC2258' as a source of resistance genes.

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“…SCAR OpD04.717 linked to QTL Phyto 5.2 which confers resistance to P. capsici (Quirin et al, 2005) was found that the genotype SCM334 resistant to P. capsici (check), which is the pepper that has greater resistance this phytopathogen (Guerrero-Moreno and Laborde, 1980;Bosland and Lindsey, 1991;Reifschneider et al, 1992;Quirin et al, 2005;Kim et al, 2008). However, in the 142 accessions was not found, therefore it was not possible to discriminate between the accessions with possibility of having resistance or susceptibility (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…El SCAR OpD04.717 ligado el QTL Phyto 5.2 que confiere resistencia a P. capsici (Quirin et al, 2005) se encontró en el genotipo resistente a P. capsici SCM334 (testigo resistente), el cual es el chile que mayor resistencia presenta a este fitopatógeno (Guerrero-Moreno y Laborde, 1980;Bosland y Lindsey, 1991;Reifschneider et al, 1992;Quirin et al, 2005;Kim et al, 2008). Sin embargo, no se encontró en las 142 accesiones, por lo tanto no fue posible discriminar entre las accesiones con posibilidad de poseer resistencia o susceptibilidad (Figura 1).…”

Section: Resultados Y Discusiónunclassified

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

Aguilar

Guerra²,

Meraz

et al. 2017

Remexca

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ResumenEn México las pérdidas en la producción de chile (Capsicum spp.) ocasionadas por infección con Phytophthora capsici Leo; han llegado a ser de hasta 100% en áreas específicas del Bajío y Puebla, por lo que se ha planteado enfrentar el problema fitosanitario mediante la búsqueda de resistencia genética en accesiones de chile serrano obtenidas a partir de colectas en diferentes regiones del país. En el presente trabajo de investigación se utilizó el marcador SCAR OpD04.717 para identificar fuentes de resistencia a dicho patógeno a nivel molecular, se consideraron las accesiones con resistencia a P. capsici cuando amplificaron una banda de 717 pb. Así también se realizaron pruebas de reacción a P. capsici bajo condiciones in vitro, con la cepa PCT-17 previamente aislada, y se obtuvo el porcentaje de supervivencia de 142 accesiones de chile más los dos testigos (SCM334, resistente y MIRASOL, susceptible al hongo). La accesión que mostró mayor resistencia a la cepa fue BGS41 en comparación con SCM334. Únicamente se identificaron accesiones de chile serrano con resistencia a P. capsici mediante pruebas de reacción in vitro. La no identificación de fuentes de resistencia al hongo a nivel AbstractIn Mexico production losses in pepper (Capsicum spp.) caused by infection of Phytophthora capsici Leo, has reached up to 100% in specific areas from the Bajio and Puebla, so it has been lay out to face the phytosanitary problem through the search of genetic resistance in serrano pepper accessions obtained from collections in different regions. In the present study the SCAR marker OpD04.717 was used to identify sources of resistance to the pathogen at molecular level, the accessions were considered resistant to P. capsici when amplified at band of 717 bp. Also reaction tests to P. capsici under in vitro conditions with strain PCT-17 previously isolated were carried out and the survival rate from 142 pepper accessions plus two checks (SCM334, resistant and MIRASOL susceptible to fungus) was obtained. The accession that showed greater resistance to strain was BGS41 compared to SCM334. Serrano pepper accessions with resistance to P. capsici were identified by in vitro test reactions. Not being able to identify sources of resistance to the fungus at molecular level in serrano pepper accessions could be due to the presence of an allele marker associated with resistance locus in a given accession / population does 1508 Rev. Mex. Cienc. Agríc. Vol.6 Núm. 7 28 de septiembre -11 de noviembre, 2015Reinaldo Méndez Aguilar et al. molecular en accesiones de chile serrano se pudo deber a que la presencia de un alelo marcador asociado con un locus de resistencia en una accesión/población determinada no implica necesariamente la misma asociación en diferentes accesiones. Se identificaron accesiones de chile serrano con utilidad potencial como nuevas fuentes de resistencia a pudriciones de la raíz (P. capsici) para programas de mejoramiento genético tradicional pero no para selección asistida por marcadores (MAS) mediante OpD04.717.Palabra...

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Inheritance of adult-plant resistance to Phytophthora capsici in pepper

Cited by 60 publications

References 12 publications

QTL Analysis for Resistance to Phytophthora capsici in Pepper Using a High Density SSR-based Map

QTL Analysis for Resistance to Phytophthora capsici in Pepper Using a High Density SSR-based Map

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

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