Inhibition by caffeic acid of the influenza A virus multiplication in vitro

Abstract

Abstract. Caffeic acid has been shown to inhibit the multiplication of influenza A virus in vitro, whereas caffeine, quinic acid and chlorogenic acid do not. Caffeic acid has also been shown to have antiviral activity against herpes simplex virus (DNA virus) and polio virus (RNA virus). In the present study, a comparison of the one-step growth curve of the influenza virus in the presence of caffeic acid with that in the absence of the reagent showed that an eclipse period of the virus multiplication in the infected cells was not affected by the reagent, while the progeny virus yield was markedly decreased in the presence of caffeic acid. In additional experiments, it was found that the addition of caffeic acid at an early time point post-infection (within 3 h post-infection) was mandatory for extensive antiviral activity, suggesting that a major target of the reagent exists in the early stages of infection. Simultaneously with the decrease in the progeny virus yield, both the virusinduced cytopathic effects and apoptotic nuclear fragmentation were markedly suppressed by the reagent, suggesting that caffeic acid suppresses, at least temporally, the degeneration of the virus-infected cells and that the observed antiviral activity is likely not the secondary result of the cytotoxic effects of the reagent. These results suggest the potential pharmacological use of caffeic acid or its derivatives as an antiviral drug against influenza A virus.

Oxidative stress, polarization of macrophages and tumour angiogenesis: Efficacy of caffeic acid

“…Recent experimental evidence suggests that caffeic acid is a potent antioxidant [10-12] and might have beneficial health impact in vivo [11,12]. Caffeic acid has several biological and pharmacological properties, such as antiviral [13], antioxidant [10-12], anti-inflammatory [8,9], anticarcinogenic [8,9,[14][15][16], and immunomodulatory activity [14].…”

Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63

“…Especially, the extract of Sambucus nigra L. exerts the antiviral activity against influenza A and B viruses, human immunodeficiency virus, and the herpes simplex virus type 1 (Krawitz et al, 2011;Serkedjieva et al, 1990;Zakay-Rones et al, 1995;Amoros et al, 1992;Mahmood et al, 1993;Roschek et al, 2009). Sambucus nigraphenolic acids like caffeic acid show the highly inhibitory effect on the in vitro replication of influenza A virus (Porter and Bode, 2017;Utsunomiya et al, 2014). Since Sambucus FormosanaNakai contains such antiviral active components in the extract of Sambucus nigra L., the antiviral activity of Sambucus FormosanaNakai is worthy to further investigate.…”

Characterization and mechanisms of anti-influenza virus metabolites isolated from the Vietnamese medicinal plant Polygonum chinense

“…2 and 3), confirming the reliability of the cell culture-based antiviral screening system. The antiviral activity of caffeic acid ( PC8 ) against influenza A virus, including H1N1 and H3N2 strains, was reported previously [25]. Herein, we evaluated the spectrum of its efficacy also against influenza B virus (Fig.…”

“…Although anti-influenza viral activity of ellagic acid ( PC5 ), methyl gallate ( PC7 ) and caffeic acid ( PC8 ) in vitro or in vivo was presented in several previous reports [23, 25, 26], their mechanisms of action have not yet been elucidated clearly except for PC8 , that targets viral NA protein [27]. To investigate their effects on virus entry and early replication in cells, time-of-addition experiment was performed (Fig.…”