Inhibitory effect of bacteriocins from enterococci on developing and preformed biofilms of Listeria monocytogenes, Listeria ivanovii and Listeria innocua

Rocha, Kátia Real; Perini, Hugo Felix; Souza, Cássia Milena de; Schueler, Janaína; Tosoni, Natara Fávaro; Furlaneto, Márcia Cristina; Furlaneto-Maia, Luciana

doi:10.1007/s11274-019-2675-0

Cited by 37 publications

(18 citation statements)

References 33 publications

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“…Propidium iodide is a membrane impermeant intercalator DNA that only stains cells with compromised membrane integrity, presenting with the reddish coloration (LEWENZA et al, 2018;ROCHA et al, 2019). Our data shows the intensity of the reddish color in the first 6 h of STEC in contact with the enterocins (Figure 2); therefore, indicating the penetration of PI.…”

Section: Resultsmentioning

confidence: 67%

“…Many of these enterocins have been tested in various food products, targeting gram-positive and gram-negative pathogens such as Listeria monocytogenes, Listeria inoccua, Bacillus cereus, Staphylococcus aureus, Clostridium sp, Klebsiella sp, Acinetobacter sp, and Pseudomonas sp (KHAN et al, 2010;ROCHA et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, it is worth mentioning that in this study we used enterocin without concentration or purification, we obtained promising results. Other studies have also reported the efficacy of unpurified enterocins against gram positive bacteria (CAMARGO et al 2016;AL ATYA et al 2016;ROCHA et al 2019).…”

Section: Resultsmentioning

confidence: 99%

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Antimicrobial activity of enterocin obtained from Enterococcus durans on Shiga-like toxin-producing Escherichia coli

et al. 2019

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Shiga-like toxin-producing Escherichia coli (STEC) is an important source of food contamination that presents risks to human health. Several industrial food processes eliminate this microorganism; however, these processes can alter the characteristics of the product. Alternative methods of preservation have been identified as an option to control these foodborne pathogens. The purpose of this study was to evaluate the action of bacteriocins produced by Enterococcus durans MF5 in STEC cells. Cell-free supernatant (CFS) containing enterocins from the MF5 isolate was tested over different time points (6, 18, and 24 h). Enterocins present in the crude CFS showed inhibition against STEC at all time points. In the investigation of cell integrity, using propidium iodide and fluorescence microscopy, considerable cell death was observed within 6 h of the cells being in contact with the enterocins, which was also observed at the 18 and 24 h time points. These results showed that the enterocins produced by the MF5 isolate have potential use in the control of STEC.

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Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

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Antimicrobial activity of enterocin obtained from Enterococcus durans on Shiga-like toxin-producing Escherichia coli

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“…A obtenção de CFS seguiu protocolo descrito por Rocha et al (2019). Um volume de 20 mL de cultivo foi centrifugado a 5000 rpm por 15 minutos e o sobrenadante teve pH ajustado em 6,5 utilizando solução de NaOH 1N e tratamento com 1mg/mL de catalase.…”

Section: Obtenção Do Sobrenadante Livre De Células E Contagem De Célulasunclassified

Milhocina como fonte proteica para produção de bacteriocina por Enterococcus faecium

Schueler

Souza

Katsuda

et al. 2021

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A produção de bacteriocina por Bactérias Ácido Lácticas tem atraído grande atenção por causa do seu status GRAS (Generally Recognized as Safe), e seu uso potencial como aditivo para a conservação de alimentos. A utilização de substrato com características que forneçam nutrientes necessários porem com reduzido custo, está entre os itens essenciais para produção e uso comercial de bacteriocinas. Portanto, o objetivo deste trabalho foi avaliar a viabilidade da produção de enterocinas, utilizando soro de leite e água de maceração de milho (milhocina) como substrato em 18 e 24 horas de cultivo. Enterococcus faecium (Efm20, Efm22, Efm24, Efm25) e Enterococcus faecalis (Efs27) foram caracterizados quanto à atividade antimicrobiana de enterocinas presentes no sobrenadante de cultivo livre de células (CFS) contra Listeria innocua CLIP 12612. O caráter proteico da substância antimicrobiana foi confirmado, sendo sua atividade inibitória perdida após o tratamento dos CFS com proteases. Em contrapartida a atividade antimicrobiana dos CFS se manteve termoestável após tratamento com calor (80°C e 100°C) e também quando tratado com catalase, o que confirma que essa atividade do CFS não está relacionada com a ação de H2O2 e ácido láctico (CFS neutralizado com NaOH). Pela contagem de células bacterianas (UFC/mL) dos isolados cultivados nos diferentes meios, observou que houve maior quantidade de UFC em MRS com substratos. A atividade antimicrobiana dos CFS obtido dos meios MRS com substrato em comparação com o MRS foram similares quando em difusão em ágar, porém ao quantificar essa atividade observou maiores valores de unidade arbitrária para os CFS obtidos em meio MRS com milhocina em ambos os tempos avaliados 18 e 24 horas. Em conclusão, constatou-se que os substratos testados apresentam grande potencial para serem aplicados na fabricação de bioconservação de produtos alimentícios.

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“…Bacteriocin (cell-free supernatant -CFS) obtaining and precipitation was performed as described by Rocha et al (2019). The pH of CFS was adjusted to 6.5 and treated with catalase, to avoid the presence of H2O2 (Ogaki et al, 2016).…”

Section: Purified Enterocin Preparation and Antagonic Testmentioning

confidence: 99%

Enterococcus faecium in artisanal ripening cheese: technological and safety aspects

Paula

Moraes

Schueler

et al. 2020

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The aims of this work were to identify and characterize for some important technological properties of the E. faecium throughout the ripening process of cheese. The study involved evaluating the technological potential of six isolates of E. faecium (EFM55, EFM67, EFM9A, EFM16A, EFM19A and EFM44) from artisanal cheeses as their probiotic characteristics and technological perspective. Concerning the antimicrobial sensibility, 71% of the E. faecium tested were sensitive to all antimicrobials. No other medicine inhibited the active of E. faecium, with the exception of dipyrone for the EFM19A and EFM9A strains. The isolates showed good antagonistic activity against gram-negative, as EFM9A, EFM55 and EFM67 strais with better activity. The highest fermentation was observed in 42 ºC, showed the pH variation of 4.15 to 5.15, after 48 hours of fermentation. All isolates showed pH reduction at 20 ºC. However, all isolates produced higher titratable acidity at a temperature of 42ºC. The isolates EFM55 and EFM9A has had probiotic actived, biochemical and susceptibility parameters desirables, aim it’s technological potential in the production of dairy products.

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Inhibitory effect of bacteriocins from enterococci on developing and preformed biofilms of Listeria monocytogenes, Listeria ivanovii and Listeria innocua

Cited by 37 publications

References 33 publications

Antimicrobial activity of enterocin obtained from Enterococcus durans on Shiga-like toxin-producing Escherichia coli

Antimicrobial activity of enterocin obtained from Enterococcus durans on Shiga-like toxin-producing Escherichia coli

Milhocina como fonte proteica para produção de bacteriocina por Enterococcus faecium

Enterococcus faecium in artisanal ripening cheese: technological and safety aspects

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