Inhibitory effects of flavonoids extracted from Nepalese propolis on the LPS signaling pathway

Funakoshi‐Tago, Megumi; Ohsawa, Kentaro; Ishikawa, Toshiyuki; Nakamura, Fumiya; Ueda, Fumihito; Narukawa, Yuji; Kiuchi, Fumiyuki; Tamura, Hiroomi; Tago, Kenji; Kimura, Tadashi

doi:10.1016/j.intimp.2016.10.008

Cited by 27 publications

(14 citation statements)

References 44 publications

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“…In vitro IKK assay. Cell lysates were prepared using NP-40 lysis were immunoprecipittated with 1 μg of an anti-IKKγ antibody and 20 μL protein G agarose (Zymed Laboratory, South San Francisco, CA) at 4 °C for 2 h. After immunoprecipitation, kinase assay was performed using 1 μg of GST-IκBα were performed as previously described 43 . To evaluate the relative IKK activity, the phosphorylation level of GST-IκBα was normalized with the amounts of GST-IκBα to show the relative IKK activity.…”

Section: Methodsmentioning

confidence: 99%

“…Extraction and separation of coffee extract and high-performance liquid chromatography (HPLC). The brewed coffee was extracted sequentially with organic solvents and the ethyl acetate extracts were further fractioned using the ODS column into three fractions: R1, R2, and R3, as described previously 43 . Figure 13.…”

Section: Extraction and Separation Of Coffee Extract And High-performmentioning

confidence: 99%

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Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

Funakoshi‐Tago

Nonaka

Tago

et al. 2020

Sci Rep

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Coffee is a complex mixture of many bioactive compounds possessing anti-inflammatory properties. However, the mechanisms by which coffee exerts anti-inflammatory effects remains unclear and the active ingredients have not yet been identified. In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that antiinflammatory activity of coffee required the roasting process. Interestingly, we identified pyrocatechol, a degradation product derived from chlorogenic acid during roasting, as the active ingredient exhibiting anti-inflammatory activity in coffee. HPLC analysis showed that 124 μM pyrocatechol was included in 100% (v/v) roasted coffee. A treatment with 5%(v/v) coffee extract and more than 2.5 μM pyrocatechol inhibited the LPS-induced activation of NF-κB and also significantly activated Nrf2, which acts as a negative regulator in LPS-induced inflammation. Furthermore, intake of 60% (v/v) coffee extract and 74.4 μM pyrocatechol, which is the concentration equal to contained in 60% (v/v) coffee, markedly inhibited the LPS-induced inflammatory responses in mice. Collectively, these results demonstrated that pyrocatechol, which was formed by the roasting of coffee green beans, is one of the ingredients contributing to the anti-inflammatory activity of coffee.

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Section: Methodsmentioning

confidence: 99%

Section: Extraction and Separation Of Coffee Extract And High-performmentioning

confidence: 99%

Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

Funakoshi‐Tago

Nonaka

Tago

et al. 2020

Sci Rep

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“…Five flavonoid components from Nepalese propolis, namely, isoliquiritigenin, chrysin, 3′,4′-dihydroxy-4-methoxydalbergione, 4-methoxydalbergion, and cearoin, exhibited anti-inflammatory activities by regulating the NF-κB pathway (Funakoshi-Tago et al. 2016). Vitexin alleviated inflammatory responses by activating important MAPK signaling pathways, such as p38, ERK1/2 and JNK (Rosa et al.…”

Section: Introductionmentioning

confidence: 99%

The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways

Tian

Chen

Chang

et al. 2019

Pharmaceutical Biology

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Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.

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“…Propolis inhibits eicosanoids and nitric oxide production, and it exhibits angiogenesis and anti-leukocyte activity, which explained its anti-inflammatory property [ 33 ]. Furthermore, propolis contains flavonoids and phenolic compounds, which have anti-inflammatory action [ 34 ]. They inhibited prostaglandin E2 production, COX-2, and mPGES-1 [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property: A novel approach in wound healing and infection

Al-Waili¹

2018

Vet World

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Aim:The study aimed to investigate whether mixing two different propolis samples can potentiate their biological activity. This hypothesis was tested by studying the effect of mixed propolis on microbial growth and wound healing and compared with the effect of each propolis individually.Materials and Methods:The effect of mixing two different propolis extracts (A and B) collected from different locations in Iraq on Escherichia coli, Staphylococcus aureus, and Candida albicans was studied by minimum inhibitory concentration assessment and compared with the effect of each propolis. Wound healing effect of the mixed propolis was studied. Twenty-four rabbits were used for the experiment, and they were assigned to four groups. Wounds were created on the dorsum of each rabbit and treated by topical application of 1 mL of either mixed propolis, propolis A, or propolis B extracts or were kept without treatment as a control. Macroscopic wound evaluation was performed with an assessment of wound size, wound recovery, redness, edema, discharge, granulation tissue, and epithelialization.Results:Propolis A was more potent than propolis B extracts to inhibit the growth of E. coli, S. aureus, and C. albicans (p<0.05). However, mixed propolis showed a higher antimicrobial activity toward all the pathogens than propolis A or propolis B extract individually (p<0.05). Furthermore, propolis A and propolis B extracts showed favorable effects on wound healing which was more pronounced with propolis A extract. Interestingly, mixed propolis accelerated wound healing faster than propolis A or propolis B extracts, and it shortened the time of reepithelialization (p<0.05).Conclusion:This study demonstrates for the first time that mixing different propolis samples possesses a higher antimicrobial activity and higher wound healing property than individual propolis. This approach could pave the way for the development of more effective antimicrobials and wound healing agents.

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Inhibitory effects of flavonoids extracted from Nepalese propolis on the LPS signaling pathway

Cited by 27 publications

References 44 publications

Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways

Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property: A novel approach in wound healing and infection

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