Initial Enzymatic Steps In The Degradation Of Alpha-Pinene By<i>Pseudomonas Fluorescens</i>Ncimb 11671

Best, D. J.; Floyd, Nicholas; Magalhães, Ana; Burfield, A.; Rhodes, P. M.

doi:10.3109/10242428709040139

Cited by 43 publications

(21 citation statements)

References 11 publications

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“…1). Studies of a-pinene metabolism from P. fluorescens NCIMB 11671 (Best et al 1987;Burfield et al 1989;Colocousi et al 1996) or Nocardia sp. P18.3 (Griffiths et al 1987a, b) reported that the enzyme catalyzing this reaction, a-pinene oxide lyase, lacked prosthetic groups and had no cofactor requirement.…”

Section: Introductionmentioning

confidence: 99%

Optimization of isonovalal production from α-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491

Fontanille

Larroche

2003

Appl Microbiol Biotechnol

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Optimization studies on the synthesis of isonovalal from alpha-pinene oxide by Pseudomonas rhodesiae CIP 107491 operated in a biphasic medium are presented. Three key parameters are identified. The first is the need for a permeabilization of cells by freezing them and then treating the thawed material with an organic solvent such as chloroform, toluene or diethyl ether. This operation allows both enzyme release into the aqueous phase outside the cells and an improvement in the transport properties of both substrate and product across the cell membrane, strongly increasing reaction rates. The second is that the enzyme alpha-pinene oxide lyase, which exhibits an irreversible inactivation by isonovalal (or a by-product), presents a constant turn-over, i.e., the total product synthesis is proportional to the biomass loading and is close to 108 mmol (16.4 g) isonovalal l(-1) g(-1) biomass. The third phenomenon is that the biphasic system used is not phase-transfer-limited, a feature attributed to the spontaneous formation of an oil-in-water emulsion. It is thus possible to carry out a very efficient process, allowing the recovery of 2.63 mol isonovalal l(-1) (400 g l(-1)) from 25 g biomass l(-1) in 2.5 h, corresponding to an average reaction rate as high as 0.70 mmol min(-1) g(-1) cells (160 g l(-1) h(-1)).

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Section: Introductionmentioning

confidence: 99%

Optimization of isonovalal production from α-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491

Fontanille

Larroche

2003

Appl Microbiol Biotechnol

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“…5). This pathway classically started by a-pinene oxygenation into a-pinene oxide followed by its decyclization into isonovalal by an a-pinene oxide lyase (Best, Floyd, Magalhaes, Burfield, & Rhodes, 1987). Isonovalal was then enzymatically converted into its trans isomer (novalal), a process active only with intact cells.…”

Section: Discussion About Metabolic Pathwaymentioning

confidence: 99%

Exploration of α-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491. Application to novalic acid production in a bioreactor

Linares

Fontanille

Larroche

2009

Food Research International

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“…In such cases, biocatalysis appears at first glance not to have any advantage over a chemical transformation. The same epoxide substrate, however, was efficiently degraded by Pseudomonas fluorescens and Nocardia strains to a small set of products belonging to one specific pathway (Best et al, 1987;Griffiths et al 1987;Fig. 4.2).…”

Section: Epoxidationmentioning

confidence: 99%

Biotechnological Flavour Generation

Berger

Krings

Zorn

2010

Food Flavour Technology

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Initial Enzymatic Steps In The Degradation Of Alpha-Pinene ByPseudomonas FluorescensNcimb 11671

Cited by 43 publications

References 11 publications

Optimization of isonovalal production from α-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491

Optimization of isonovalal production from α-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491

Exploration of α-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491. Application to novalic acid production in a bioreactor

Biotechnological Flavour Generation

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