Insights into soy lecithin and egg yolk-based extenders for chilling canine spermatozoa

Dalmazzo, Andressa; Angrimani, Daniel de Souza Ramos; Losano, João Diego de Agostini; Rocha, Carolina Camargo; Sobrinho, C. A. Baptista; Gurgel, João Rafael Chinait; Pacheco, Pedro Ivo Monteiro; Minazaki, Cláudia Kiyomi; Crusco, Silvia Edelweiss; Nichi, Marcílio; Barnabé, Valquíria Hyppólito

doi:10.1017/s0967199418000576

Cited by 7 publications

(13 citation statements)

References 43 publications

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“…However, increasing the concentration of soy lecithin to 1% led to promising results on post-thaw semen viability and acrosome integrity in the current study, which is in agreement with previous studies [16,29,30]. This finding supports previous suggestions that high concentrations of soy lecithin are necessary to maintain canine sperm characteristics (i.e., motility kinetics) during storage [28]. Besides that, the type of soy lecithin used as a base in the extender seems to be very important, since different lecithin products contain different concentrations of phosphatidylcholine [16], which has an important role in incorporating choline in the polar region of spermatozoa plasma membrane and plays an active role in sperm capacitation and acrosome reaction.…”

Section: Discussionsupporting

confidence: 93%

“…Soy lecithin can also serve as an antioxidant and can protect semen from oxidative stress resulting in higher viability and motility after cryopreservation [16]. A recent study in dogs showed that soy lecithin at low concentrations (0.01%, 0.05%, and 0.1%) was not able to maintain sperm characteristics when compared to egg-yolk-based extenders [28]. However, increasing the concentration of soy lecithin to 1% led to promising results on post-thaw semen viability and acrosome integrity in the current study, which is in agreement with previous studies [16,29,30].…”

Section: Discussionmentioning

confidence: 99%

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Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Pipan

Casal

Šterbenc

et al. 2020

Animals

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Simple Summary: Soy lecithin and sucrose were used at different concentrations to develop and compare different vitrification methods for the cryopreservation of canine semen. All of the sperm quality characteristics were effectively preserved after devitrification when vitrification extenders containing soy lecithin at 1% and 0.25 M sucrose were used. The results suggest that vitrification is effective, fast, and simple for cryopreservation of canine semen. Furthermore, the use of soy lecithin in lieu of animal proteins (e.g., egg yolk) facilitates semen shipping to countries with strict import requirements.Abstract: A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 × 10 6 spermatozoa/mL. From each group, a 33 µL (1.65 × 10 6 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Pipan

Casal

Šterbenc

et al. 2020

Animals

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“…Our findings revealed that among the test concentration range of SL (0.5-2%), the best concentration of SL for cryopreservation of rooster semen was 1.0%. However, previous sperm studies for semen cryopreservation in other species demonstrated that the optimal SL concentration ranged from 0.8 % in canines [9], 1.5% in bulls [8], 2% in rams [7], and 6% in boars [28]. This inconsistency may be related to species-specific differences.…”

Section: Discussionmentioning

confidence: 93%

“…It also acts as an antioxidant against free radical damage, resulting in improved viability and motility after cryopreservation. Many studies confirm the beneficial impact of SL for semen cryopreservation in bulls [8], rams [7], canines [9], bears [10], and so on. A previous study from our laboratory demonstrated that 20% egg yolk and 2.0% SL added to the extenders produced similar protective effects on goat semen cryopreservation [11].…”

Section: Introductionmentioning

confidence: 86%

Beneficial Influence of Soybean Lecithin Nanoparticles on Rooster Frozen–Thawed Semen Quality and Fertility

Sun

et al. 2021

Animals

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The present study aimed to investigate the impact of different concentrations (0%, 0.5%, 1.0%, 1.5%, and 2.0%) of nano-soybean lecithin (SL) in the extender on sperm quality, sperm motion characteristics, and fertility outcomes of post-thawed rooster semen. Adult Ross broiler breeder roosters (n = 20) were subjected to semen collections twice a week for three weeks. At each collection, semen samples were pooled and allocated into five treatments corresponding to different nano-SL concentrations (control, SL0.5, SL1.0, SL1.5, and SL2.0). Sperm parameters, including motility (collected using a computer-assisted sperm analysis system), plasma membrane and acrosome integrities, and mitochondrial activity were assessed. Sperm malondialdehyde (MDA) and antioxidant activities (total antioxidant capacity (TAC); superoxide dismutase (SOD); glutathione peroxidase (GPx)) were evaluated. The fertility and hatchability obtained with frozen–thawed rooster semen supplemented with the optimum nano-SL concentration were assessed after artificial insemination. The results showed that the addition of 1% nano-SL into the extender led to a higher semen motility in roosters, improved plasma membrane and acrosome integrities, and higher mitochondrial activity of post-thawed rooster semen in comparison to controls (p < 0.05). The MDA levels in the SL0.5 and SL1.0 groups were lower than the other groups (p < 0.05). TAC activities in SL0.5, SL1.0, and SL1.5 groups were significantly higher than those in the other groups (p < 0.05). It was observed that the concentration of SOD was higher in the SL1.0 group than in the other groups (p < 0.05). The activity of GPx was not influenced in any of the cases (p > 0.05). Moreover, the percentages of fertility and hatchability in the SL1.0 group were higher (56.36% and 58.06%) than those in the control group (42.72% and 40.43%). In summary, the addition of nano-SL to the extenders enhanced the post-thawed semen quality and fertility of roosters by reducing the level of oxidative stress. The optimum nano-SL concentration was 1.0%. These results may be beneficial for improving the efficacy of semen cryopreservation procedures in poultry breeding.

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“…The delivery system of micelles is composed of a mixture of surfactants, lecithin, and natural antioxidants (Li et al, 2017). Increasing evidence shows that the extraction of lecithin from egg yolk has potential health-enhancing antioxidant properties involving superoxide anion and hydroxyl radical scavenging and ferrous chelation (Dalmazzo et al, 2019). Another study demonstrated that the antioxidant activity of lecithin emulsifier significantly influenced the permeation of free radicals across the emulsion interface (Chung et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Wedelolactone-Loaded Micelles Ameliorate Doxorubicin-Induced Oxidative Injury in Podocytes by Improving Permeability and Bioavailability

Feng

Wang

et al. 2019

Front. Bioeng. Biotechnol.

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Wedelolactone (WED) is commonly used for the treatment of doxorubicin (DOX)-induced kidney damage, but its efficacy is limited by its poor solubility and bioavailability. In this study, we developed a novel delivery system of WED-loaded micelles (WED-M) with Solutol® HS15 and lecithin at an optimized ratio of 7:3 to improve the poor permeability and bioavailability of WED and to enhance its efficacy. The spherically shaped WED-M (particle size: 160.5 ± 3.4 nm; zeta potential: −30.1 ± 0.9 mV; entrapment efficiency: 94.41 ± 1.64%; drug loading: 8.58 ± 0.25%; solubility: 1.89 ± 0.06 mg/ml) has continuous stability over 14 days and a sustained release profile. The permeability of WED-M in Caco-2 cells indicated a significant 1.61-fold higher Papp AP to BL ratio than WED alone. Additionally, pharmacokinetic evaluation of WED-M demonstrated that the bioavailability of WED was increased 2.78-fold. Both HE staining and transmission electron microscopy showed an obvious improvement of pathological damage in WED-M treatment. Moreover, WED-M significantly enhanced the ROS level in mice and MPC5 podocytes. We concluded that using this micelle delivery system for WED could improve its permeability and bioavailability to attenuate DOX-induced oxidative injury in podocytes. This study provided important information on the fact that the micelle delivery system, WED-M, showed a significant improvement of renal damage.

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Insights into soy lecithin and egg yolk-based extenders for chilling canine spermatozoa

Cited by 7 publications

References 43 publications

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Beneficial Influence of Soybean Lecithin Nanoparticles on Rooster Frozen–Thawed Semen Quality and Fertility

Wedelolactone-Loaded Micelles Ameliorate Doxorubicin-Induced Oxidative Injury in Podocytes by Improving Permeability and Bioavailability

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