Interaction between integrin α5 and fibronectin is required for metastasis of B16F10 melanoma cells

Feng, Qian; Zhang, Zi-Chao; Wu, Xuefeng; Li, Yupei; Xu, Qiang

doi:10.1016/j.bbrc.2005.06.039

Cited by 93 publications

(102 citation statements)

References 19 publications

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“…Consistent with our previous observations in transiently interfered cells (Sciamanna et al, 2005), the expression of cyclin D1 and c-myc is downregulated in L1 stably interfered cells. Consistent with our prediction, the differentiation markers respond variably to L1 RNAi: a-5 integrin, whose expression positively correlates with melanoma metastatic progression (Quia et al, 2005), is downregulated after L1 RNAi; in contrast, mda-7, whose expression increases during induction of terminal differentiation while being expressed at low levels, if at all, in tumor cells (Dent et al, 2005), is upregulated (Figure 3c). …”

Section: Resultssupporting

confidence: 84%

Distinct roles for LINE-1 and HERV-K retroelements in cell proliferation, differentiation and tumor progression

et al. 2007

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Transformed cells express high levels of non-telomeric reverse-transcriptase (RT) activity of retrotransposon and endogenous retrovirus origin. We previously reported that RT inhibition, either pharmacological or through transient silencing of RT-encoding LINE-1 (L1) elements by RNA interference (RNAi), reduced proliferation, induced differentiation and reprogrammed gene expression in human tumorigenic cell lines. Moreover, the antiretroviral drug efavirenz antagonized tumor progression in animal models in vivo. To get insight into the role of retroelements in tumorigenesis, we have now produced two cell lines derived from A-375 melanoma, in which the expression of either L1 retrotransposon, or HERV-K endogenous retrovirus, was stably suppressed by RNAi. Compared to the parental A-375 cell line, cells with stably interfered L1 expression show a lower proliferation rate, a differentiated morphology and lower tumorigenicity when inoculated in nude mice. L1 silencing modulates expression of several genes and, unexpectedly, also downregulates HERV-K expression. In HERV-K interfered cells, instead, L1 expression was unaffected, and cell proliferation and differentiation remained unchanged compared to parental A-375 cells. In vivo, however, their tumorigenic potential was found to be reduced after inoculation in nude mice. These results suggest that L1 and HERV-K play specific and distinct roles in cell transformation and tumor progression.

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Section: Resultssupporting

confidence: 84%

Distinct roles for LINE-1 and HERV-K retroelements in cell proliferation, differentiation and tumor progression

et al. 2007

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“…Changes in tumor phenotype associated with drug-resistance have been associated with metastatic potential [20,21]. For example, interaction between integrin α 5 and fibronectin is necessary for metastasis of B16 murine melanoma cells [22]. TG2 in the ECM functions as a co-receptor for both integrins and fibronectin.…”

Section: Discussionmentioning

confidence: 99%

Tissue transgluaminase 2 expression in meningiomas

Behdad

Siegel

et al. 2008

J Neurooncol

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Meningiomas are common intracranial tumors that occur in extra-axial locations, most often over the cerebral convexities or along the skull-base. Although often histologically benign these tumors frequently present challenging clinical problems. Primary clinical management of patients with symptomatic tumors is surgical resection. Radiation treatment may arrest growth or delay recurrence of these tumors, however, meningioma cells are generally resistant to apoptosis after treatment with radiation. Tumor cells are known to alter their expression of proteins that interact in the ECM to provide signals important in tumor progression. One such protein, fibronectin, is expressed in elevated levels in the ECM in a number of tumors including meningiomas. We recently reported that levels of both extracellular fibronectin and tissue transglutaminase 2 (TG2) were increased in glioblastomas. We examined the expression of fibronectin and its association TG2 in meningiomas. Both fibronectin and TG2 were strongly expressed in all meningiomas studied. TG2 activity was markedly elevated in meningiomas, and TG2 was found to co-localize with fibronectin. Treatment of meningiomas with the small molecule TG2 inhibitor, KCC009, inhibited the binding of TG2 to fibronectin and blocked disposition of linear strands of fibronectin in the ECM. KCC009 treatment promoted apoptosis and enhanced radiation sensitivity both in cultured IOMM-Lee meningioma cells and in meningioma tumor explants. These findings support a potential protective role for TG2 in meningiomas.

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“…15 On the other hand, Xu et al have shown that integrin a5 promotes melanoma metastasis through enhancing cell adhesion to extracellular matrix Fibronectin and protects melanoma cells from apoptosis. 16 Based on these observations we decided to study whether the antimetastatic effect of Pentoxifylline, is at least in part, mediated by its effect on adhesion and cell surface integrin receptors. So in this study we looked at the effect of PTX on integrin expression on B16-F10 cells and integrin mediated adhesion of B16-F10 melanoma cells.…”

Section: Introductionmentioning

confidence: 99%

Pentoxifylline modulates cell surface integrin expression and integrin mediated adhesion of B16F10 cells to extracellular matrix components

Ratheesh¹,

Ingle²,

Gude³

2007

Cancer Biology & Therapy

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Key woRdsPentoxifylline, adhesion, integrins, metastasis, B16-F10 melanoma, a5b1 integrin, Protein Kinase C AbbRevIAtIons AbstRActOur previous studies demonstrated that Pentoxifylline (PTX), a phosphodiesterase inhibitor, could inhibit the lung homing of B16-F10 melanoma cells in C57BL/6 mice. In this study we have looked at the effect of PTX on cell surface integrin expression and integrin mediated adhesion of B16-F10 melanoma cells. B16-F10 cells treated with PTX when injected through the tail vein of mice showed a 75% reduction in pulmonary nodules as compared to control untreated cells. PTX brought about a significant reduction in the integrin mediated adhesion of F10 cells to Fibronectin and Vitronectin (58.75% ± 3.4 S.E and 60% ± 1.7 S.E respectively if control was considered as 100%). This inhibition in adhesion was evident up to four hours only and treatment for 24 hours brought about an increase in adhesion (135.5% ± 0.5 S.E). Flow cytometric analysis showed higher surface expressions of av, a5 and aIIb integrin subunits in B16-F10 as compared to the low metastatic cell line B16-F1 suggesting a role for these integrins in determining the metastatic potential. PTX brought about a significant decrease in the cell surface expression of a5, aIIb and b1integrin subunits but not that of the av subunit on B16-F10 cells. PTX also brought about a reduction in the total cellular protein levels of b1 and av integrin subunits. Various isoforms of Protein Kinase C (PKC) has been shown to regulate integrin expression, localization and activity. Hence we looked at the effect of PTX on total cellular PKC activity. PTX brought about a significant reduction in total cellular PKC activity (82.66 ± 0.593). Collectively our results indicate that the antimetastatic action of PTX is mediated, at least in part through its effects on adhesion and the surface expression of specific integrin receptors.

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Interaction between integrin α5 and fibronectin is required for metastasis of B16F10 melanoma cells

Cited by 93 publications

References 19 publications

Distinct roles for LINE-1 and HERV-K retroelements in cell proliferation, differentiation and tumor progression

Distinct roles for LINE-1 and HERV-K retroelements in cell proliferation, differentiation and tumor progression

Tissue transgluaminase 2 expression in meningiomas

Pentoxifylline modulates cell surface integrin expression and integrin mediated adhesion of B16F10 cells to extracellular matrix components

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