Interactions Between Arabidopsis Thaliana and Phytopathogenic Pseudomonas Pathovars: A Model for the Genetics of Disease Resistance

Dangl, Jeff; Lehnackers, Hiltrud; Kiedrowski, Siegrid; Debener, Thomas; Rupprecht, Christoph; Martin, Aaron; Somssich, Imre E.

doi:10.1007/978-94-015-7934-6_12

“…Arabidopsis thaliana plants were maintained as described by Dangl et al (1991) and Debener et al (1991). Conditions for pea (Vivian et al, 1989) and bean (Harper et al, 1987) have also been described previously.…”

Section: Care Of Plantsmentioning

confidence: 99%

“…The molecular genetic advantages of Arabidopsis (RBdei, 1975;Meyerowitz, 1987Meyerowitz, , 1989 have been recently exploited to investigate the genetic control of plant-pathogen interactions (Susnova and Poljak, 1975;Melcher, 1989;Slusarenko, 1990a, 1990b;Li and Simon, 1990;Simpson and Johnson, 1990;Ausubel et al, 1991;Bent et al, 1991;Dangl et al, 1991Dangl et al, , 1992Daniels et al, 1991;Davis et al, 1991;Debener et al, 1991;Dong et al, 1991;Simons et al, 1991;Tsuji et al, 1991;Whalen et al, 1991;Uknes et al, 1992; reviewed by Dangl, 1992b). Two approaches have been taken.…”

Section: Introductionmentioning

confidence: 99%

Functional homologs of the Arabidopsis RPM1 disease resistance gene in bean and pea.

Dangl

¹

,

Ritter

²

,

Gibbon

³

et al. 1992

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We showed that a bacterial avirulence (avr) gene function, avrPpiA1, from the pea pathogen Pseudomonas syringae pv pisi, is recognized by some, but not all, genotypes of Arabidopsis. Thus, an avr gene functionally defined on a crop species is also an avr gene on Arabidopsis. The activity of avrPpiA1 on a series of Arabidopsis genotypes is identical to that of the avrRpm1 gene from P.s. pv maculicola previously defined using Arabidopsis. The two avr genes are homologous and encode nearly identical predicted products. Moreover, this conserved avr function is also recognized by some bean and pea cultivars in what has been shown to be a gene-for-gene manner. We further demonstrated that the Arabidopsis disease resistance locus, RPM1, conditioning resistance to avrRpm1, also conditions resistance to bacterial strains carrying avrPpiA1. Therefore, bean, pea, and conceivably other crop species contain functional and potentially molecular homologs of RPM1.

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“…Several groups have recently shown that Arabidopsis may also be well suited as a model system to study plant-pathogen interactions [ 14,15,16]. Therefore, we cloned the counterpart genes from Arabidopsis, as elucidation of the functions encoded by these genes should be facilitated using this plant species.…”

mentioning

confidence: 99%

“…In this respect it is noteworthy that recent experiments have shown At-eli 3 to be strongly activated upon infiltration of Arabidopsis leaves with avirulent Pseudomonas strains [15]. In addition, sequence comparison of the translated region of the At-eli 3 gene with that of the parsley ELI 3 cDNA showed high sequence similarity throughout (S. Kiedrowski et al, manuscript in preparation), demonstrating that our cloning strategy had indeed resuited in the isolation of corresponding genes very likely encoding similar or identical functions.…”

mentioning

confidence: 99%

Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells

Trezzini

¹

,

Horrichs

²

,

Somssich

³

1993

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Numerous Arabidopsis genes have been cloned that correspond to putative pathogen defense-related genes identified in parsley (Petroselinum crispum). Treatment of Arabidopsis cells with fungal elicitor leads to rapid accumulation of the respective mRNAs with time courses comparable to those observed for their counterparts in parsley. Evolutionary sequence conservation of many of these genes in several plant species suggests they code for important plant functions.

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“…The molecular genetic advantages of Arabidopsis (RBdei, 1975;Meyerowitz, 1987Meyerowitz, , 1989 have been recently exploited to investigate the genetic control of plant-pathogen interactions (Susnova and Poljak, 1975;Melcher, 1989;Slusarenko, 1990a, 1990b;Li and Simon, 1990;Simpson and Johnson, 1990;Ausubel et al, 1991;Bent et al, 1991;Dangl et al, 1991Dangl et al, , 1992Daniels et al, 1991;Davis et al, 1991;Debener et al, 1991;Dong et al, 1991;Simons et al, 1991;Tsuji et al, 1991;Whalen et al, 1991;Uknes et al, 1992; reviewed by Dangl, 1992b). Two approaches have been taken.…”

Section: Introductionmentioning

confidence: 99%

Functional Homologs of the Arabidopsis RPM1 Disease Resistance Gene in Bean and Pea

Dangl¹,

Ritter²,

Gibbon³

et al. 1992

View full text Add to dashboard Cite

We showed that a bacterial avirulence (avr) gene function, avrPpiA1, from the pea pathogen Pseudomonas syringae pv pisi, is recognized by some, but not all, genotypes of Arabidopsis. Thus, an avr gene functionally defined on a crop species is also an avr gene on Arabidopsis. The activity of avrPpiA1 on a series of Arabidopsis genotypes is identical to that of the avrRpm1 gene from P.s. pv maculicola previously defined using Arabidopsis. The two avr genes are homologous and encode nearly identical predicted products. Moreover, this conserved avr function is also recognized by some bean and pea cultivars in what has been shown to be a gene-for-gene manner. We further demonstrated that the Arabidopsis disease resistance locus, RPM1, conditioning resistance to avrRpm1, also conditions resistance to bacterial strains carrying avrPpiA1. Therefore, bean, pea, and conceivably other crop species contain functional and potentially molecular homologs of RPM1.

show abstract

Interactions Between Arabidopsis Thaliana and Phytopathogenic Pseudomonas Pathovars: A Model for the Genetics of Disease Resistance

Cited by 11 publications

References 9 publications

Functional homologs of the Arabidopsis RPM1 disease resistance gene in bean and pea.

Functional homologs of the Arabidopsis RPM1 disease resistance gene in bean and pea.

Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells

Functional Homologs of the Arabidopsis RPM1 Disease Resistance Gene in Bean and Pea

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