Interferon System of Human Cornea Cells: Interferon Production, Characterization, and Development of Antiviral State

Thacore, Harshad R.; Mount, David T.; Chadha, Kailash C.

doi:10.1089/jir.1982.2.401

Cited by 11 publications

(5 citation statements)

References 22 publications

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“…Both poly(IC) and exogenous IFN (4--7, t3, 14) have been found iu clinical trials to be very effective therapeutic agents in the treatment of ophthalmic herpes infections. These clinical observations axe compatible with the recent in.vitro finding that IFN-produetioa as well as the establishment of an antiviraI state can be efficiently induced in human cornea cells (15). Viral eye diseases are frequently accompanied by bacterial infections, mos@ involving hyaluronidase producing strains (10).…”

Section: Poly(ri) Poly(rc) [Poly(ic)] Is a Potent Interferon (Ifn) Isupporting

confidence: 82%

Effect of hyaluronidase on cell response to the antiviral and interferon inducing activity of poly(rI) � poly(rC)

Romano

Ladijinsky

Aboud

1983

Archives of Virology

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The effect of hyaluronidase on cell response to poly(rI) . poly(rC) [poly(IC)] was investigated in rabbit kidney (RK) cells. For this purpose, bovine testicular and Staphylococcal hyaluronidase preparations at various degrees of purity were used. These enzyme preparations were employed at the maximal nontoxic dose for 2 hours before poly(IC) treatment. This enzymatic pretreatment of the cells strongly inhibited the antiviral activity of poly(IC), determined by using both Herpes simplex virus type-1 and vesicular stomatitis virus. It also remarkably reduced the poly(IC)-induced interferon production. This later effect could account for the diminished antiviral activity of poly(IC) in the hyaluronidase-treated cells.

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Section: Poly(ri) Poly(rc) [Poly(ic)] Is a Potent Interferon (Ifn) Isupporting

confidence: 82%

Effect of hyaluronidase on cell response to the antiviral and interferon inducing activity of poly(rI) � poly(rC)

Romano

Ladijinsky

Aboud

1983

Archives of Virology

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“…Human foreskin (BG-9), African green monkey kidney (CV-1) and murine L-929 cells were grown and maintained in Eagle's MEM, as described previously Thacore et al, 1982).…”

Section: Methodsmentioning

confidence: 99%

Effect of Protein Kinase C Inhibitors on Interferon-beta Production by Viral and Non-viral Inducers

Thacore¹,

Lin²,

Davis

et al. 1990

Journal of General Virology

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Induction of interferon-fl (IFN-fl) in human (BG-9), simian (CV-1) and mouse (L-929) cell lines by Sendai virus and by poly(rI), poly(rC) has been studied for its possible dependence on protein kinase C (PKC) through the use of pharmacological inhibitors (K252a and H-7) of PKC. Exposure of BG-9, CV-1 or L-929 cells to K252a (>10.025 ~tM), a staurosporine derivative, 24 h before or after induction of IFN with poly(rl).poly(rC), inhibited by > 95 % the production of IFN-fl. In contrast, virus-induced 1FN production was enhanced threefold or more by K252a in BG-9 and L-929 but not in CV-1 cells. A naphthalene sulphonamide inhibitor of PKC, H-7, at >/5 ~tM, decreased poly(rI).poly(rC)-induced IFN production in BG-9 and CV-1 cells by 75 to 94%, but had no effect on IFN production in L-929 cells. Viral induction of IFN was not affected significantly by H-7 in BG-9, CV-1 and L-929 cells. In contrast to these results, the calmodulin inhibitor, trifluoperazine (5 to 15 ~tM) did not affect IFN-fl production by poly(rI).poly(rC) but significantly enhanced IFN production by Sendai virus in both human and murine cell lines. Thus, in human and simian fibroblasts the induction of IFN-fl by poly(rI).poly(rC) appears to be PKC-dependent, whereas viral induction of IFN-fl is not. Results with K252a implicate PKC in non-viral induction of IFN in mouse fibroblasts, as well. Direct measurements of PKC activity in BG-9 cells exposed to several concentrations of K252a showed that the membrane PKC activity is significantly more sensitive to inhibition by K252a than is cytosolic PKC activity. In L-929 cells, K252a inhibited membrane PKC activity similarly, but was less effective as an inhibitor of cytosolic enzyme activity than in BG-9. These studies support an intregral role for PKC activity, particularly membraneassociated activity, in non-viral [poly(rI).poly(rC)] induction of IFN-fl in human, simian and mouse fibroblasts.

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“…18,19 IFN-a is secreted by leucocytes, IFN-b by fibroblasts, and IFN-g by T cells and natural killer (NK) cells. INFs also stimulate production of major histocompatibility complex (MHC) class I molecules and proteins that enhance the ability of virally infected cells to present viral proteins to T cells.…”

mentioning

confidence: 99%

Immune defense at the ocular surface

Akpek

Gottsch

2003

Eye

111

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The ocular surface is constantly exposed to a wide array of microorganisms. The ability of the outer ocular system to recognize pathogens as foreign and eliminate them is critical to retain corneal transparency, hence preservation of sight. Therefore, a combination of mechanical, anatomical, and immunological defense mechanisms has evolved to protect the outer eye. These host defense mechanisms are classified as either a native, nonspecific defense or a specifically acquired immunological defense requiring previous exposure to an antigen and the development of specific immunity. Sightthreatening immunopathology with autologous cell damage also can take place after these reactions. This article discusses the innate and acquired corneal elements of the immune defense at the ocular surface. The relative roles of the various factors contributing to prevention of eye infection remain to be fully defined. Eye (2003) 17, 949-956.

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Interferon System of Human Cornea Cells: Interferon Production, Characterization, and Development of Antiviral State

Cited by 11 publications

References 22 publications

Effect of hyaluronidase on cell response to the antiviral and interferon inducing activity of poly(rI) � poly(rC)

Effect of hyaluronidase on cell response to the antiviral and interferon inducing activity of poly(rI) � poly(rC)

Effect of Protein Kinase C Inhibitors on Interferon-beta Production by Viral and Non-viral Inducers

Immune defense at the ocular surface

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