Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model

Ishiguro, Hiroyuki; Kaito, Takashi; Yarimitsu, Seido; Hashimoto, Kunihiko; Okada, Rintaro; Kushioka, Junichi; Chijimatsu, Ryota; Takenaka, Shota; Makino, Takahiro; Sakai, Yusuke; Moriguchi, Yu; Otsuru, Satoru; Hart, David A.; Fujie, Hiromichi; Nakamura, Norimasa; Yoshikawa, Hideki

doi:10.1016/j.actbio.2019.01.050

Cited by 51 publications

(59 citation statements)

References 28 publications

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“…MSCs also demonstrated the potential in regenerative medicine by not only providing improvement in behavioral assays and paracrine effects but also directing cellto-cell interactions that resulted in protection against neurovascular damage in a stroke (Ryu et al, 2019). Moreover, stem cells and/or their derivatives have the potential to regenerate cartilage, bone, heart, and liver (Chetty, Praneetha, Govarthanan, Verma, & Vadivel Murugan, 2019;Hong et al, 2018;Ishiguro et al, 2019;Kuraitis, Ruel, & Suuronen, 2011). Clearly, stem cells have tremendous potential that can be harnessed to treat many diseases and structural damages using cell therapy and regenerative medicine strategies.…”

Section: Opportunitiesmentioning

confidence: 99%

Mesenchymal stem cells: Cell therapy and regeneration potential

Brown

McKee

Bakshi

et al. 2019

J Tissue Eng Regen Med

448

310

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Rapid advances in the isolation of multipotent progenitor cells, routinely called mesenchymal stromal/stem cells (MSCs), from various human tissues and organs have provided impetus to the field of cell therapy and regenerative medicine. The most widely studied sources of MSCs include bone marrow, adipose, muscle, peripheral blood, umbilical cord, placenta, fetal tissue, and amniotic fluid. According to the standard definition of MSCs, these clonal cells adhere to plastic, express cluster of differentiation (CD) markers such as CD73, CD90, and CD105 markers, and can differentiate into adipogenic, chondrogenic, and osteogenic lineages in vitro. However, isolated MSCs have been reported to vary in their potency and self‐renewal potential. As a result, the MSCs used for clinical applications often lead to variable or even conflicting results. The lack of uniform characterization methods both in vitro and in vivo also contributes to this confusion. Therefore, the name “MSCs” itself has been increasingly questioned lately. As the use of MSCs is expanding rapidly, there is an increasing need to understand the potential sources and specific potencies of MSCs. This review discusses and compares the characteristics of MSCs and suggests that the variations in their distinctive features are dependent on the source and method of isolation as well as epigenetic changes during maintenance and growth. We also discuss the potential opportunities and challenges of MSC research with the hope to stimulate their use for therapeutic and regenerative medicine.

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Section: Opportunitiesmentioning

confidence: 99%

Mesenchymal stem cells: Cell therapy and regeneration potential

Brown

McKee

Bakshi

et al. 2019

J Tissue Eng Regen Med

448

310

View full text Add to dashboard Cite

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“…In the terminal phase of IVD degradation, collagenous fibers in the AF become weak or even ruptured, and the degraded NP matrix can herniate through the weakened point in the AF, stimulating and/or constrict the spinal cord. 117 Traditional treatment for IVD degradation involves removing the herniated tissue followed by fusion surgery on the adjacent vertebrae to stabilize the segment and limit motion. However, the operation sacrifices segmental flexibility while still retaining the risk of re-herniation.…”

Section: Use Of Genipin In Skeletal System Tissue Regenerationmentioning

confidence: 99%

Regeneration of skeletal system with genipin crosslinked biomaterials

et al. 2020

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Natural biomaterials, such as collagen, gelatin, and chitosan, are considered as promising candidates for use in tissue regeneration treatment, given their similarity to natural tissues regarding components and structure. Nevertheless, only receiving a crosslinking process can these biomaterials exhibit sufficient strength to bear high tensile loads for use in skeletal system regeneration. Recently, genipin, a natural chemical compound extracted from gardenia fruits, has shown great potential as a reliable crosslinking reagent, which can reconcile the crosslinking effect and biosafety profile simultaneously. In this review, we briefly summarize the genipin extraction process, biosafety, and crosslinking mechanism. Subsequently, the applications of genipin regarding aiding skeletal system regeneration are discussed in detail, including the advances and technological strategies for reconstructing cartilage, bone, intervertebral disc, tendon, and skeletal muscle tissues. Finally, based on the specific pharmacological functions of genipin, its potential applications, such as its use in bioprinting and serving as an antioxidant and anti-tumor agent, and the challenges of genipin in the clinical applications in skeletal system regeneration are also presented.

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“…Unfortunately, surgical interventions suffer limitations, and their failure to induce regeneration or stop IVD degeneration is the main drawback. To overcome the inherent restrictions associated with surgical methods to cure LBP, recent investigations have been focused on IVD regenerative medicine with growing interests in cell, biomolecules, and gene therapies [ 19 , 20 , 21 , 22 , 23 ]. Despite intense research interest, attempts to regenerate the IVD have failed so far and no effective strategy has translated into a successful clinical outcome [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

Tavakoli

Diwan

Tipper

2020

IJMS

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Damage to the annulus fibrosus (AF), the outer region of the intervertebral disc (IVD), results in an undesirable condition that may accelerate IVD degeneration causing low back pain. Despite intense research interest, attempts to regenerate the IVD have failed so far and no effective strategy has translated into a successful clinical outcome. Of particular significance, the failure of strategies to repair the AF has been a major drawback in the regeneration of IVD and nucleus replacement. It is unlikely to secure regenerative mediators (cells, genes, and biomolecules) and artificial nucleus materials after injection with an unsealed AF, as IVD is exposed to significant load and large deformation during daily activities. The AF defects strongly change the mechanical properties of the IVD and activate catabolic routes that are responsible for accelerating IVD degeneration. Therefore, there is a strong need to develop effective therapeutic strategies to prevent or reconstruct AF damage to support operational IVD regenerative strategies and nucleus replacement. By the way of this review, repair and regenerative strategies for AF reconstruction, their current status, challenges ahead, and future outlooks were discussed.

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Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model

Cited by 51 publications

References 28 publications

Mesenchymal stem cells: Cell therapy and regeneration potential

Mesenchymal stem cells: Cell therapy and regeneration potential

Regeneration of skeletal system with genipin crosslinked biomaterials

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

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