Intracellular Distribution of Amines Taken up by Rat Mast Cells In Vitro

Bergendorff, Anders

doi:10.1111/j.1748-1716.1975.tb10035.x

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…There are any number of concerns with this explanation. Firstly, there is major con troversy as to whether peritoneal mast cells are cap able of taking up histamine [26,27], Second, these authors contend that the specific serotonin uptake inhibitor fluoxetine [28] could permit differential release and quote a previous paper [23] as having also done so, while in that paper fluoxetine was reported not to permit differential release. Moreover, a newer specific serotonin uptake inhibitor, sertraline [29], also did not permit differential release [unpubl.…”

Section: Discussionmentioning

confidence: 96%

Progesterone Triggers Selective Mast Cell Secretion of 5-Hydroxytryptamine

Vliagoftis

Dimitriadou

Theoharides³

1990

Int Arch Allergy Immunol

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Mast cells are involved in allergic reactions where they secrete numerous mediators in response to immunoglobulin E and antigen. However, they have recently been implicated in neuroinflammatory conditions with a higher prevalence in women, and there have been clinical reports of progesterone anaphylaxis. When tested on purified rat peritoneal mast cells, progesterone alone stimulated release only of 5-hydroxy-tryptamine (serotonin) in a dose- and time-dependent manner. Serotonin release by progesterone was exceptional because it was not accompanied by histamine release or degranulation and was either augmented or unaffected by drugs which inhibit secretion induced by the classic mast cell secretagogue, compound 48/80. These findings indicate that mast cells are capable of selective serotonin secretion, previously shown only after pretreatement with certain tricyclic drugs, and may be involved in neuroendocrine syndromes.

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Section: Discussionmentioning

confidence: 96%

Progesterone Triggers Selective Mast Cell Secretion of 5-Hydroxytryptamine

Vliagoftis

Dimitriadou

Theoharides³

1990

Int Arch Allergy Immunol

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“…Mast cells contain one of the largest depots of histamine found in mammalian cells 43 . Dopamine, like histamine, belongs to the monoamine neurotransmitter family and have similar requirements for their storage and uptake 44 .…”

Section: Discussionmentioning

confidence: 99%

SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo

Pettersson

Zarnegar

Westin

et al. 2017

Sci Rep

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Mast cells act as sensors in innate immunity and as effector cells in adaptive immune reactions. Here we demonstrate that SLC10A4, also referred to as the vesicular aminergic-associated transporter, VAAT, modifies mast cell degranulation. Strikingly, Slc10a4 −/− bone marrow-derived mast cells (BMMCs) had a significant reduction in the release of granule-associated mediators in response to IgE/antigen-mediated activation, whereas the in vitro development of mast cells, the storage of the granule-associated enzyme mouse mast cell protease 6 (mMCP-6), and the release of prostaglandin D2 and IL-6 were normal. Slc10a4-deficient mice had a strongly reduced passive cutaneous anaphylaxis reaction and a less intense itching behaviour in response to the mast cell degranulator 48/80. Live imaging of the IgE/antigen-mediated activation showed decreased degranulation and that ATP was retained to a higher degree in mast cell granules lacking SLC10A4. Furthermore, ATP was reduced by two thirds in Slc10a4 −/− BMMCs supernatants in response to IgE/antigen. We speculate that SLC10A4 affects the amount of granule-associated ATP upon IgE/antigen-induced mast cell activation, which affect the release of granule-associated mast cell mediators. In summary, SLC10A4 acts as a regulator of degranulation in vitro and of mast cell-related reactions in vivo.

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“…Mast cells, which are found in all anatomical structures of the body except the blood and lymph, possess one of the largest contents of the biogenic amines histamine and serotonin (S-hydroxytryptamine) found within mammalian cells [ 1,2]. These amines, along with large amounts of the anionjc mucopolysaccharide heparin and proteolytic enzymes, are stored in high concentration within the osmophilic storage granules of the mast cell [3,4].…”

Section: Introductionmentioning

confidence: 99%