Intraspecific Variation and Host Specificity of Entomophthora muscae sensu stricto Isolates Revealed by Random Amplified Polymorphic DNA, Universal Primed PCR, PCR-Restriction Fragment Length Polymorphism, and Conidial Morphology

Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) using specific primers

“…The six distinct restriction enzymes tested in this study were able to clearly distinguish the different Metarhizium species investigated. Ribosomal genes and their ITS and IGS spacer regions have been widely used for the identification and differentiation of species (Fouly et al, 1997) as well as in taxonomic (Driver et al, 2000), phylogenetic (Rakotonirainy et al, 1994) and genetic diversity (Anderson et al, 2001;Uetake et al, 2002) studies, with ITS sequences having been reported as being useful for discriminating between different species of fungi (Neuveglise et al, 1994;Fouly et al, 1997;Jensen et al, 2001;Anderson et al, 2001;Thomsen and Jensen, 2002). 250 Destéfano et al Figure 4 -Amplification of DNA from pure cultures and infected larvae using the primer sets ITSMet/ITS4 (A) and ITS14/ITS4 (B).…”

“…Techniques which use DNA probes to create restriction fragment length polymorphism (RFLP) fingerprints have been used to distinguish between species (Hegedus and Khachatourians, 1993a) or individual isolates (Hegedus and Khachatourians, 1993b), although direct DNA probing methods may not exhibit the desired degree of sensitivity required for detection under field conditions (Hegedus and Khachatourians, 1996b). Other methods based on the polymerase chain reaction (PCR), such as the random amplified polymorphic DNA (RAPD) method, also exhibit the ability to discriminate between entomopathogenic fungal isolates (Jensen et al, 2001;Freire et al, 2001;Alves et al, 2001;Urtz and Rice, 1997;Fungaro et al, 1996;Leal et al, 1994;Bidochka et al, 1994) but the use of random oligonucleotide primers in the design of these systems is not useful in the detection of fungi within environmental samples which can contain a significant amount of DNA from indigenous biotic materials.…”

Biological Control of Parasites

“…The entomophthorales are another group of fungi that are able to cause natural outbreaks in insect populations and are also promising as good BCAs [56]. Several different Entomophthora muscae sensu stricto genotypes have been documented, and each type has demonstrated a high degree of host specificity [56].…”

“…Several different Entomophthora muscae sensu stricto genotypes have been documented, and each type has demonstrated a high degree of host specificity [56]. All available literature deal with E. muscae as a pathogenic fungi of adult Musca domestica, but Khater [53] isolated, for the first time in Egypt, from Moshtohor, Toukh and Qlubia governorate a strain that has the unique ability to infect larvae of the house flies.…”

Ecosmart Biorational Insecticides: Alternative Insect Control Strategies

“…Several different Entomophthora muscae sensu stricto genotypes were documented and each type was restricted to a single host species, indicating a very high degree of host specificity at or below the level of the subfamily ( Jensen et al, 2001). Currently, no commercially-based product is available because of difficulties in mass production.…”