Investigation of diversity of plasmids carrying the blaTEM-52 gene

Abstract

The bla(TEM-52) gene found in humans could have been transmitted on transferable plasmids originating from animal sources. Some of the bla(TEM-52) plasmids carry replicons that differ from the classical ones. Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor bla(TEM-1), the bla(TEM-52) gene was not detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

“…Evidence for this exists through a straightforward analysis of the historical ECOR and SAR reference collections, which reveal a great deal of diversity among their plasmids and an abundance of plasmids in strains of both collections (116). Indeed, a variety of plasmid types have been found among Salmonella isolates, including those of incompatibility (Inc) types IncA/C, IncF, IncH, IncI, IncL/M, IncN, IncR, and IncX (117)(118)(119)(120)(121)(122). What seems to have changed over time is the accessory gene content that these plasmids carry.…”

Complete Nucleotide Sequence of Two Multidrug-Resistant IncR Plasmids from Klebsiella pneumoniae

“…ince the first description of the novel IncR complex replicons in 2009 (1), multidrug resistance (MDR) plasmids containing IncR characteristic sequences and carrying multiple resistance genes, such as bla , bla VIM-1 , bla DHA-1 , bla TEM-52 , qnrS1, or armA, have been increasingly reported in Enterobacteriaceae isolates from various geographical regions (1)(2)(3)(4)(5)(6)(7). Moreover, IncR replicons have been found either as single replicons (7) or as part of multireplicon plasmids, i.e., plasmids harboring multiple replication initiation proteins (6,(8)(9)(10).…”

Characterization ofblaTEM-52-Carrying Plasmids of Extended-Spectrum-β-Lactamase-Producing Salmonella enterica Isolates from Chicken Meat with a Common Supplier in Japan

“…Another plasmid, pE001, also was found to show high similarity with pYM4, with 38,602/38,614 nucleotides found to be identical (with 10 gaps) in a BLASTn search. pE001 was identified in 2006 in an E. coli strain from broiler meat in Denmark (9). A comparison of the sequences indicated that the bla gene of pYM4 belongs to the TEM-52b allele, and the Tn3 transposon is located upstream of the bla gene.…”

“…bla TEM-52 was initially identified from Klebsiella pneumoniae in France, 1996 (10). Several studies also reported bla TEM-52 -positive Salmonella from livestock, meat, and humans in European countries, suggesting the dissemination of the resistance gene (1)(2)(3)(4)9). The plasmid is a major vehicle of resistance.…”