Investigation of wound healing activity of Lafoensia pacari (Lythraceae) leaves extract cultivated in Goiás state, Brazil

Abstract

Lafoensia pacari has been used in traditional folk medicine in Brazilian Cerrado to treat wounds. It is important to develop studies that can clarify how the biological activity of L. pacari occurs. The aim of this study was to evaluate the healing activity of L. pacari leaves extract in an animal model. The extract was prepared from plants cultivated in Goiania-GO, Brazil. The healing activity was assayed using cutaneous wound model in rats, and macroscopic, morphometric and histological analysis of wounds were also conducted. The presence of hydrolysable tannins was detected in thin layer chromatography and in high performance liquid chromatography analysis, may be suggested the presence of ellagitannins. In the evaluation of cutaneous wounds in rats it was possible to observe that the treatment with 10% (w/v) L. pacari extract provided a reduction in the time of cutaneous wound healing, with a significant increase in variables involved in healing, such as the number of blood vessels and collagen production. Therefore, this study shows that L. pacari wound healing potential may be related to the presence of ellagitannins and corroborates to ethnopharmacological reports regarding this plant.

Atividade antimicrobiana do extrato, frações e punicalagina da casca do fruto de Punica granatum frente a isolados clínicos de vacas com mastite

“…Typically, the α and β anomeric structures of the hydrolyzable tannins show up as two peaks in the chromatograms (Chaibub et al, 2020). Thus, the chromatographic profile of the P. granatum extract showed two intense peaks with a retention time of 13.214 min and 14.132 min, respectively (Figure 3A).…”

“…The stationary phase was a Waters XTerra® C18 column (250 x 4.6 mm, 5 µm), injection volume 10 µL, operating temperature 40 °C, mobile phase flow 1.0 mL/min, detection at wavelength 260 nm and elution time of 25 min. The mobile phase was composed of acetonitrile (A) and 0.01 M (v/v) phosphate buffer (B), using the following elution gradient: 5% A and 95% B in 5 min; 15% A and 85% B in 10 min; 30% A and 70% B in 16 min; 3% A and 97% B in 18 min and 3% A and 97% B in 25 min as described by Chaibub et al (2020), with adaptations. After purification by column chromatography, the structural elucidation of punicalagin was determined by 1D and 2D Nuclear Magnetic Resonance (NMR) in a Bruker Avance III 500 spectrometer, operating at a frequency of 500.13 MHz for 1 H and 125.76 MHz for the 13 C.…”

Exploring Cheminformatic Toolsets for Predicting the Dermal Toxicity of Furanocoumarins

“…In any case, most authors support that LF physicochemical features are of utmost importance to their effects, being structural characteristics like the condensed aromatic system often hinted as pharmacophores in both natural and synthetic components [29,[49][50][51][52], hence particular physicochemical and pharmacological attributes [53][54][55]. Moreover, this core moiety confers enough lipophilicity to these chemicals to allow their diffusion through the skin and cross other biological barriers such as the blood-brain barrier [56].…”