Involvement of peroxidases in the coffee resistance to orange rust (Hemileia vastatrix)

Silva, Maria do Céu; Guerra‐Guimarães, Leonor; Loureiro, Andreia; Nicole, Michel

doi:10.1016/j.pmpp.2008.04.004

Cited by 48 publications

(61 citation statements)

References 40 publications

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“…These authors showed activation of various isozymes of POX in infected wheat plants and concluded that the increase in POX activity was a consequence and not the determinant of resistance. Silva et al (2008) demonstrated the involvement of POX in the resistance of coffee to orange rust Hemileia vastatrix and reported peak POX activity during an oxidative burst and hypersensitive response in the cells. Gogoi et al (2001) detected an increase in POX activity following inoculation of wheat with Neovossia indica.…”

Section: Discussionmentioning

confidence: 96%

Peroxidase and polyphenol oxidase activities in compatible host–pathogen interaction inJasminum officinaleandUromyces hobsoni: Insights into susceptibility of host

Kuvalekar

Redkar

Gandhe

et al. 2011

New Zealand Journal of Botany

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Uromyces hobsoni Vize. is a rust fungus which infects Jasminum officinale var. grandiflorum. The fungus is mainly observed on the stem and leaves in the form of raised galls at the site of infection. Healthy and infected progressive disease stages on stem and leaves were collected directly from the field and were analysed for the activity of defence enzymes like peroxidase and polyphenol oxidase along with the estimation of total phenols. There was no significant alteration in the activity of polyphenol oxidase and peroxidase in stem tissues, although the leaves exhibited a significant increase in the activity of these enzymes at advanced disease stages. A significant increase in the amount of phenolics was observed at early and advanced stages of disease in leaves and stem, respectively. These studies correlate the susceptibility of the host with the concentration of preformed antimicrobial compounds and the temporal activity of defence enzymes during progress of the disease.

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Section: Discussionmentioning

confidence: 96%

Peroxidase and polyphenol oxidase activities in compatible host–pathogen interaction inJasminum officinaleandUromyces hobsoni: Insights into susceptibility of host

Kuvalekar

Redkar

Gandhe

et al. 2011

New Zealand Journal of Botany

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“…A rota dos fenilpropanóides, iniciada pela conversão da L-fenilalanina a ácido trans-cinâmico, numa reação de deaminação catalisada pela enzima FAL, é de suma importância para a produção de compostos fenólicos de natureza antimicrobiana, além de certas classes de fitoalexinas (Schuster & Rétey, 1995). As POX estão relacionadas com o processo de proteção antioxidativa catalisando a oxidação de componentes celulares como o peróxido de hidrogênio e também no aumento da síntese de lignina que fortalece a parede celular contra a ação de enzimas líticas produzidas em abundância por alguns patógenos (Silva et al, 2008). As PFO catalisam a hidroxilação de monofenóis formando orto-difenóis e também a oxidação de ortodifenóis formando orto-quinonas, as quais exibem natureza antimicrobiana superior aos compostos fenólicos que as precedem (Valero et al, 1988).…”

Section: Resultsunclassified

Efeito da aplicação foliar de silício na resistência à ferrugem e na potencialização da atividade de enzimas de defesa em cafeeiro

Pereira¹,

Rodrigues²,

Carré‐Missio³

et al. 2009

Trop. plant pathol.

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Parte da Dissertação de Mestrado da primeira autora. Universidade Federal de Viçosa. Viçosa MG. 2007. RESUMOA principal medida de controle da ferrugem do cafeeiro, causada por Hemileia vastatrix, é o uso de fungicidas. O fornecimento de silício (Si) às plantas é uma estratégia de controle interessante considerando o potencial desse elemento em aumentar a resistência de várias espécies de plantas à patógenos. Este trabalho avaliou o efeito da aplicação foliar do Si na redução da severidade da ferrugem e na possível potencialização da atividade de seis enzimas relacionadas com a resistência de plantas à patógenos. Mudas de café (cultivar Catuaí Vermelho 44) foram pulverizadas com água destilada, solução de silicato de potássio (KSi) (35 g/L, pH 10,5), KSi (35 g/L, pH 5,5) e solução de acibenzolar-S-metil (ASM) (200 µg/L) 24 horas antes da inoculação. Não houve diferença significativa entre os tratamentos para os teores foliares de Si e potássio. A severidade foi significativamente maior em mudas pulverizadas com água em relação aos demais tratamentos. O ASM reduziu significativamente a severidade em 70% em relação à aplicação de água. A aplicação de soluções de KSi, independente do pH, também reduziu a severidade. O ASM foi eficiente em aumentar a atividade de quitinases e β-1,3-glucanases, porém a aplicação de KSi, independente do pH da solução, foi ineficiente em potencializar a atividade das seis enzimas estudadas, embora tenha reduzido a severidade. Palavras-chave: Hemileia vastatrix, mecanismo bioquímico de defesa, nutrição mineral. ABSTRACT Effect of foliar silicon application on resistance against coffee leaf rust and on the potentiation of defense enzymes in coffeeThe major method for control of coffee leaf rust, caused by Hemileia vastatrix, is the use of fungicides. The supply of silicon (Si) to plants is an interesting control strategy because this element can increase the resistance of many plant species to pathogens. This study aimed to evaluate the effect of foliar application of Si on reducing rust severity as well as to determine if it can potentiate the activity of enzymes related to host resistance to pathogens. Coffee plants (cultivar Catuaí Vermelho 44) were sprayed with distilled water, potassium silicate (KSi) solution (35 g/L, pH 10.5), KSi (35 g/L, pH 5.5), and acibenzolar-S-methyl (ASM) solution (200 µg/L) 24 hours before inoculation. There was no significant difference among the treatments regarding the content of Si and potassium in the leaf tissue. Rust severity was significantly higher on plants sprayed with water as compared to the other treatments. The ASM significantly decreased rust severity by 70% in comparison to the water spray treatment. Foliar application of KSi, regardless of the pH, was efficient in decreasing rust severity. The ASM was efficient in increasing the activity of chitinases and β-1,3-glucanases. On the other hand, the application of β-1,3-glucanases. On the other hand, the application of -1,3-glucanases. On the other hand, the application of KSi, regardless of t...

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“…Although the activity of antioxidative enzyme GPOD was increased after infection of R. solanacearum, the activity of enzyme was rapidly increased in R cultivar Arka Shirish followed by S cultivar MEBH-11 and HS cultivars Green Round in early time intervals of challenge inoculated. Defense-related enzymes GPOD, PPO, PAL, and LOX are known to play a major role biosynthesis of cell wall strengthening material and secretion of antimicrobial compounds such as phytoalexins, furanocoumarin, quinones, and pterocarpan [21] which determine the host resistance. Similar results were exhibited when Cerebroside elicitors initiate accumulation of H 2 O 2 followed by the production of plant defense-related enzymes viz., PAL, POX, PPO, and LOX in Capsicum annuum L. Similarly, Increase in the GPOD activity was observed during the interaction between tomato and R. solanacearum, rice and Xanthomonas oryzae pv.…”

Section: Gpod Activitymentioning

confidence: 99%

“…Silva et al [21] revealed the increased GPOD activity in relation to host cell wall lignifications in coffee resistance to orange rust.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Molecular Variations of Guaiacol Peroxidase and Total Phenols in Bacterial Wilt Pathogenesis of Solanum melongena

Prakasha¹,

Umesha²

2016

Biochem Anal Biochem

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Plants respond to bacterial pathogen attack by activating various defense responses, which are associated with the accumulation of several factors like defense-related enzymes and inhibitors which serve to prevent pathogen infection. The present study focused on the role of the defense-related enzyme and gene expression of Guaiacol preroxidase and Total phenol content in imparting activities was analyzed by selecting three different eggplant cultivars against bacterial wilt pathogen Ralstonia solanacearum. The temporal pattern of induction of these enzymes showed (42.72 U) maximum activity at 21 h after the pathogen inoculation (hpi) in resistant cultivars. The expressions of defense genes increased 5.5 folds in resistant eggplant cultivars after pathogen inoculation. The total phenol content increased significantly (P<0.05) in resistant cultivars upon pathogen inoculation compared to susceptible and highly susceptible cultivars. The biochemical and molecular markers provided an insight to understand the first line of defense responses in eggplant cultivars upon inoculation with the pathogen.

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Involvement of peroxidases in the coffee resistance to orange rust (Hemileia vastatrix)

Cited by 48 publications

References 40 publications

Peroxidase and polyphenol oxidase activities in compatible host–pathogen interaction inJasminum officinaleandUromyces hobsoni: Insights into susceptibility of host

Peroxidase and polyphenol oxidase activities in compatible host–pathogen interaction inJasminum officinaleandUromyces hobsoni: Insights into susceptibility of host

Efeito da aplicação foliar de silício na resistência à ferrugem e na potencialização da atividade de enzimas de defesa em cafeeiro

Biochemical and Molecular Variations of Guaiacol Peroxidase and Total Phenols in Bacterial Wilt Pathogenesis of Solanum melongena

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