Ion chromatography as reference method for serum cations

An improved reference method for serum cations measurement by ion chromatography

“…All elements, including the internal standard (rubidium, Rb), can be determined in a single measurement within 30 minutes, as depicted in Figure . In the chromatogram, the peaks are complete baseline separated from each other, although the concentrations of the cations differ by more than 2 orders of magnitude, which are similar to Schiel's report . However, according to the results of our experiments, the IonPac CS16 analytical column may have more advantages for the separation of alkaline earth metal and ammonium than IonPac CS12 or IonPac CS12A analytical column which is chosen for disparating concentration ratios of adjacent eluting cations such as sodium and ammonium in diverse sample matrices.…”

“…Therefore, the serum specimens should be treated before injecting to the ion chromatography system for separation and analysis. Thienpont and coworkers have done a lot of work in the determination of serum cations by ion chromatography . According to their previous work, the serum specimens were simply treated by acidic dilution to dilute the sample matrix and release the doubly charged cations from the proteins.…”

“…In the chromatogram, the peaks are complete baseline separated from each other, although the concentrations of the cations differ by more than 2 orders of magnitude, which are similar to Schiel's report. 13 However, according to the results of our experiments, the IonPac CS16 analytical column may have more advantages for the separation of alkaline earth metal and ammonium than IonPac CS12 or IonPac CS12A analytical column which is chosen for disparating concentration ratios of adjacent eluting cations such as sodium and ammonium in diverse sample matrices. Besides, no impurity peak can be observed in the serum chromatogram, indicating the presence of other cations has no influence on the determination of Na, K, Ca, and Mg under the selected condition.…”

“…However, those methods were normally very elaborate and time‐consuming and not suitable for every metrological task. Thienpont et al first reported that the ion chromatography could be recommended as reference method for the determination of serum cations in clinical chemistry. However, according to their previous work, the serum specimens were simply treated by acidic dilution to dilute the sample matrix and release the doubly charged cations from the proteins.…”

Advanced analytical methods and sample preparation for ion chromatography techniques

“…The detector detects components which are different in property from the eluent and gives signals in proportion to the concentration for a substance of a few micrograms or less. 17,[41][42][43] However hydroxide eluent is difficult to use because it readily absorbs carbon dioxide and forms carbonate. 14,39 The recording system records the intensities of the signals obtained by the detector.…”